EC Number   |
|---|
   5.4.99.9 | - |
| 5.4.99.9 | HQ20 column chromatography and HP-20 column chromatography |
| 5.4.99.9 | HQ20 column chromatography and HP-20 hydrophobic interaction chromatography column |
| 5.4.99.9 | nickel immobilized affinity chromatography and DEAE column chromatography |
| 5.4.99.9 | nickel immobilized metal affinity chromatography, DEAE column chromatography, and purification with HaloLink resin |
| 5.4.99.9 | Protino Ni-IDA 1000 column chromatography |
| 5.4.99.9 | recombinant C-terminally His6-tagged enzyme from Escherichia coi strain BL21 StarTM (DE3) by nickel affinity chromatography |
| 5.4.99.9 | recombinant expression of His6-tagged or GSG-tagged enzyme by nickel affinity chromatography and gel filtration |
| 5.4.99.9 | recombinant GST-tagged enzyme from Escherichia coli strain BL21 (DE3) pLysS or B893 (DE3) pLysS by glutathione affinity chroatography and gel filtration |
| 5.4.99.9 | recombinant His8-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography, tag cleavage with TEV protease, dialysis, and another step of nickel affinity chromatography and dialysis |
