EC Number   |
|---|
    4.2.1.84 | - |
| 4.2.1.84 | (NH4)2SO4 fractionation |
| 4.2.1.84 | (NH4)2SO4 fractionation, ion-exchange/hydrophobic/gel-filtration chromatography, stabilization by n-butyric acid, yield of purified protein: 23.96% |
| 4.2.1.84 | 3 successive column chromatographies in the presence of 40 mM n-butyric acid (only wild type), alphaQ90N mutein is purified in nitrosylated state in the dark |
| 4.2.1.84 | ammonium sulfate fractionation and chromatography on Q-Sepharose, phenyl-Sepharose and Sephadex G150 |
| 4.2.1.84 | ammonium sulfate fractionation, chromatography on DEAE-Cellulofine, phenyl-Sepharose, Sephadex G-150 and octyl-Sepharose, 11.5fold purification, N-774 |
| 4.2.1.84 | ammonium sulfate fractionation, chromatography on DEAE-Sephacel and phenyl-Sepharose CL-4B and gel filtration, R312 strain, 10.2fold purified |
| 4.2.1.84 | ammonium sulfate fractionation, chromatography on DEAE-Sephacel, octyl and phenyl-Sepharose CL-4B |
| 4.2.1.84 | ammonium sulfate fractionation, chromatography on DEAE-Sephacel, octyl-Sepharose CL-4B and phenyl-Sepharose CL-4B, 4.91fold purification |
| 4.2.1.84 | ammonium sulfate fractionation, chromatography on DEAE-Sephacel, phenyl-Sepharose and Sephacryl S-300, 3.31fold purification |
