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EC Number
Purification (Commentary)
4.2.1.78
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4.2.1.78
1590fold to homogeneity from cell suspension culture
4.2.1.78
4-isozymes, partial
4.2.1.78
HisTrap Fast Flow column
4.2.1.78
HisTrap Fast Flow column equilibrated with a 50 mM Tris HCl buffer at pH 7.5. A linear gradient of imidazole concentration from 0 to 0.5 M (buffered at pH 7.5) is applied
4.2.1.78
Ni-NTA affinity column chromatography
4.2.1.78
nickel resin column chromatography
4.2.1.78
recombinant C-terminally His-tagged NCS from Eschericha coli strain BL21(DE3) by nickel affinity chromatography
4.2.1.78
recombinant His-tagged enzyme
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