EC Number   |
|---|
    4.2.1.104 | B |
| 4.2.1.104 | co-purified with thiocyanate hydrolase of Thiohalophilus thiocyanoxidans, gel filtration and SDS-PAGE |
| 4.2.1.104 | partial |
| 4.2.1.104 | precipitation, chromatography |
| 4.2.1.104 | recombinant CynS-MBP from Escherichia coli strain HMS174 by amylose affinity chromatography |
| 4.2.1.104 | recombinant His-tagged enzyme from Escherichia coli BL21(DE3) pLys by nickel affinity chromatography and dialysis |
| 4.2.1.104 | recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography, dialysis, and ultrafiltration, recombinant MBP-tagged enzyme from Escherichia coli strain BL21(DE3) by affinity chromatography, dialysis in presence of 5 mm maltose, ultrafiltration, and gel filtration |
| 4.2.1.104 | recombinant His-tagged soluble CYN from Escherichia coli strain BL21(DE3) by nickel affinity chromatography |
| 4.2.1.104 | recombinant N-terminally His6-tagged enzyme from Escherichia coli strain Rosetta (DE3) or Trichoplusia ni High Five cells by nickel affinity chromatography, anion and/or cation ion exchange chromatography, and gel filtration to over 90% purity |
