EC Number   |
|---|
    4.1.3.1 | - |
| 4.1.3.1 | 76fold |
| 4.1.3.1 | 90% homogeneity |
| 4.1.3.1 | ammonium sulfate frationation, gel filtration, ion exchange chromatography (DEAE-cellulose) |
| 4.1.3.1 | ammonium sulfate precipitation, DEAE cellulose column chromatography, and Sephadex G-25 gel filtration |
| 4.1.3.1 | ammonium sulfate precipitation, phenyl-Sepharose column chromatography, and Resource Q column chromatography |
| 4.1.3.1 | anion exchange chromatography (DEAE), immobilized metal ion affinity chromatography (Ni2+), gel filtration |
| 4.1.3.1 | copurified with aconitase |
| 4.1.3.1 | ecombinant His-tagged wild-type and mutant enzymes from Escherichia coli strain BL21(DE3) by nickel affinity chromatography |
| 4.1.3.1 | from overproducing strain |
