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Search Purification (Commentary)

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EC Number Purification (Commentary) Reference
Display the reaction diagram Show all sequences 3.5.1.122native enzyme from a brain extract by ion exchange, hydrophobic chromatography, and gel filtration 741003
Display the reaction diagram Show all sequences 3.5.1.122recombinant N-terminally His6-MBP-tagged enzyme from Escherichia coli strain B834 as Se-Met labeled protein by nickel affinity chromatography, tag cleavage by TEV protease, gel filtration,and another step of nickel affinity chromatography, followed by desalting gel filtration 741295
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