EC Number   |
|---|
   3.4.25.1 | - |
| 3.4.25.1 | 20S proteasome complex |
| 3.4.25.1 | ammonium sulfate precipitation, Q-Sepharose column chromatography, CHTII hydroxyapatite column chromatography, and phenyl-Sepharose column chromatograpyh |
| 3.4.25.1 | anti-Flag M2 agarose bead chromatography, and gel filtration |
| 3.4.25.1 | DEAE column chromatography, MonoQ HR5/5 column chromatography, and Superose 6 gel filtration |
| 3.4.25.1 | DEAE-Toyopearl 650S column chromatography and Superose 6 gel filtration |
| 3.4.25.1 | development of a method to purtify the 26S proteasome intact from whole Arabidopsis seedlings |
| 3.4.25.1 | development of an affinity method to rapidly purify intact epitope-tagged 26S proteasomes using mutant homozygous PAG1-FLAG pag1-1 line |
| 3.4.25.1 | development of an integrated proteomic approach, QTAX, for quantitative analysis of tandem affinity purified in vivo cross-linked protein complexes to capture protein interactions of all natures in a single analysis, overview. Investigation of cell cycle specific proteasome interaction networks. Wild-type and RPN11-HBH cells are synchronized in three phases (G1, S, and M) before cross-linking and tandem affinity purification using nickel affinity resin and streptavidin beads after lysis using buffer containing 8 M urea, 300 mM NaCl, 50 mM NaH2PO4, 0.5% Igepal, 20 mM imidazole, and 1 mM PMSF, pH 8.5 |
| 3.4.25.1 | fungal 26S proteasome, comprising 102 distinct proteins, by anion exchange chromatography and gel filtration, method optimization, mass spectrometry and protein identifications, overview |
