EC Number   |
|---|
   3.4.22.3 | - |
| 3.4.22.3 | a major ficin from the commercial crude proteinase mixture preparation of Ficus carica is purified by using a SP-Sepharose cation-exchanger column on fast protein liquid chromatography |
| 3.4.22.3 | combination of cation-exchange chromatography on SP-Sepharose Fast Flow, Thiopropyl Sepharose 4B and FPLC-gel filtration chromatography |
| 3.4.22.3 | commercial preparation |
| 3.4.22.3 | ficin II and III |
| 3.4.22.3 | further purification of a commercial latex preparation by cation exchange chromatography, and 2,2'-dipyridyl disulfide-glutathione or aminophenylmercuric acetate affinity chromatography, or by Gly-Phe-NH-methylcyanide affinity chromatography with elution by 2,2'-dipyridyl disulfide |
| 3.4.22.3 | multiple molecular forms |
| 3.4.22.3 | SP-Sepharose column chromatography |
| 3.4.22.3 | three-phase partitioning is applied for purification and recovery of ficin from Ficus carica latex. The enzyme is purified to 6.04fold with 167% recovery |
| 3.4.22.3 | using HPLC chromatography, ultrafiltration, ion-exchange chromatography and gel filtration |
