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Information on EC 3.4.22.3 - ficain
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3.4.22.3 ficainSpecify your search results
Word Map
- 3.4.22.3
- papain
- bromelain
- proteinases
- cathepsins
- chymotrypsin
- ficus
- cystatins
- latex
- alpha-chymotrypsin
-
alloantibody
- chymopapain
-
phytocystatins
-
active-centre
- actinidin
- analysis
-
antiglobulin
- medicine
- food industry
- synthesis
-
2,2\'-dipyridyl
- industry
The enzyme appears in viruses and cellular organisms
Synonyms
ficin, ficin a, ficain, ficin e, 
more
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SYNONYM 
ORGANISM
UNIPROT
COMMENTARY 
LITERATURE
debricin
-
-
-
-
EC 3.4.4.12
-
-
formerly
-
ficin
ficin A
ficin B
ficin C
ficin E
higueroxyl Delabarre
-
-
-
-
ficin
-
-
-
-
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REACTION
REACTION DIAGRAM
COMMENTARY
ORGANISM
UNIPROT
LITERATURE
Similar to that of papain
-
-
-
-
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REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
hydrolysis of peptide bond
hydrolysis of peptide bond
-
-
endopeptidase
-
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CAS REGISTRY NUMBER
COMMENTARY
9001-33-6
-
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SUBSTRATE
PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate)
(Substrate)
LITERATURE
(Substrate)
(Substrate)
COMMENTARY
(Product)
(Product)
LITERATURE
(Product)
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
r=reversible
ir=irreversible
?=not specified
1-methylpiperidin-4-one + 4-nitrobenzaldehyde
?
-
reaction condition: 30°C, MeCN (0.85 mL) and deionized water (0.15 mL). Yield: 44%, 19:81 anti/syn ratio, enantioselectivity: 5%/1% (anti/syn)
-
-
?
acetyl-citrulline-p-nitroanilide + H2O
acetyl-citrulline + 4-nitroaniline
Ficus sp.
-
30% of the activity with benzoyl-DL-Arg-p-nitroanilide
-
-
?
benzoyl-citrulline-p-nitroanilide + H2O
benzoyl-citrulline + 4-nitroaniline
Ficus sp.
-
81% of the activity with benzoyl-DL-Arg-p-nitroanilide
-
-
?
benzoyl-DL-Arg-p-nitroanilide + H2O
benzoyl-DL-Arg + 4-nitroaniline
Ficus sp.
-
-
-
-
?
benzoyl-DL-Arg-p-nitroanilide hydrochloride + H2O
benzoyl-DL-Arg + 4-nitroaniline hydrochloride
-
-
-
-
?
benzoyl-L-arginine 4-nitroanilide + H2O
benzoyl-L-arginine + 4-nitroaniline
Ficus sp.
-
-
-
-
?
benzoyl-Phe-Val-Arg-NH-(4-nitrophenyl) + H2O
benzoyl-Phe-Val-Arg + 4-nitrophenol
-
-
-
-
?
benzoyl-Phe-Val-Arg-NH-7-amido-4-methylcoumarin + H2O
benzoyl-Phe-Val-Arg + 7-amino-4-methylcoumarin
-
-
-
-
?
benzyloxycarbonyl-Ala-methyl ester + Ala
benzyloxycarbonyl-Ala-Ala + ?
-
-
-
?
benzyloxycarbonyl-Ala-methyl ester + Ala
benzyloxycarbonyl-Ala-D-Gln + ?
-
-
-
?
benzyloxycarbonyl-Ala-methyl ester + Cys(acetamidomethyl)
benzyloxycarbonyl-Ala-Cys(acetamidomethyl) + ?
-
-
-
?
benzyloxycarbonyl-Arg-Arg-7-amido-4-methylcoumarin + H2O
benzyloxycarbonyl-Arg-Arg + 7-amino-4-methylcoumarin
-
-
-
-
?
benzyloxycarbonyl-citrulline-p-nitroanilide + H2O
benzyloxycarbonyl-citrulline + 4-nitroaniline
Ficus sp.
-
75% of the activity with benzoyl-DL-Arg-p-nitroanilide
-
-
?
benzyloxycarbonyl-D-Ala-methyl ester + Ala
benzyloxycarbonyl-Ala-D-Ala + ?
-
-
-
?
benzyloxycarbonyl-Gln-methyl ester + Ala
benzyloxycarbonyl-Ala-Gln + ?
-
-
-
?
benzyloxycarbonyl-Gly-citrulline-p-nitroanilide + H2O
?
Ficus sp.
-
11% of the activity with benzoyl-DL-Arg-p-nitroanilide
-
-
?
benzyloxycarbonyl-Gly-Gly-Phe-citrulline-p-nitroanilide + H2O
?
Ficus sp.
-
activity is 2.97fold higher than with benzoyl-DL-Arg-p-nitroanilide
-
-
?
benzyloxycarbonyl-Gly-methyl ester + Ala
benzyloxycarbonyl-Gly-Ala + ?
-
-
-
?
benzyloxycarbonyl-Gly-methyl ester + Cys(acetamidomethyl)
benzyloxycarbonyl-Gly-Cys(acetamidomethyl) + ?
-
-
-
?
benzyloxycarbonyl-Gly-methyl ester + Gln
benzyloxycarbonyl-Gly-Gln + ?
-
-
-
?
benzyloxycarbonyl-Gly-Phe-citrulline-p-nitroanilide + H2O
?
Ficus sp.
-
activity is 12.6fold higher than with benzoyl-DL-Arg-p-nitroanilide
-
-
?
benzyloxycarbonyl-Gly-Pro-citrulline-p-nitroanilide + H2O
?
Ficus sp.
-
39% of the activity with benzoyl-DL-Arg-p-nitroanilide
-
-
?
benzyloxycarbonyl-Phe-Arg-7-amido-4-methylcoumarin + H2O
benzyloxycarbonyl-Phe-Arg + 7-amino-4-methylcoumarin
-
-
-
-
?
benzyloxycarbonyl-Phe-Arg-NH-(4-nitrophenyl) + H2O
benzyloxycarbonyl-Phe-Arg + 4-nitrophenol
-
-
-
-
?
benzyloxycarbonyl-Phe-Arg-NH-7-amido-4-methylcoumarin + H2O
benzyloxycarbonyl-Phe-Arg + 7-amino-4-methylcoumarin
-
-
-
-
?
benzyloxycarbonyl-Phe-citrulline-p-nitroanilide + H2O
?
Ficus sp.
-
activity is 19.4fold higher than with benzoyl-DL-Arg-p-nitroanilide
-
-
?
benzyloxycarbonyl-Pro-citrulline-p-nitroanilide + H2O
?
Ficus sp.
-
23% of the activity with benzoyl-DL-Arg-p-nitroanilide
-
-
?
Boc-Gln-Ala-Arg-7-amido-4-methylcoumarin + H2O
Boc-Gln-Ala-Arg + 7-amino-4-methylcoumarin
-
-
-
-
?
cyclohexanone + 3-nitrobenzaldehyde
?
-
reaction condition: 30°C, MeCN (0.85 mL) and deionized water (0.15 mL). Yield: 39%, 86:14 anti/syn ratio, enantioselectivity: 81%/14% (anti/syn)
-
-
?
cyclohexanone + 4-cyanobenzaldehyde
?
-
reaction condition: 30°C, MeCN (0.85 mL) and deionized water (0.15 mL). Yield: 21%, 79:21 anti/syn ratio, enantioselectivity: 77%/5% (anti/syn)
-
-
?
insulin B chain + H2O
fragments of insulin B chain
-
hydrolysis of 6 bonds
-
-
?
N-acetyl-D-Phe-Gly 4-nitroanilide + H2O
N-acetyl-D-Phe-Gly + 4-nitroaniline
Ficus sp.
-
the index of stereochemical selectivity for N-acetyl-L-Phe-Gly 4-nitroanilide and N-acetyl-D-Phe-Gly 4-nitroanilide is 93
-
-
?
N-acetyl-L-Phe-Gly 4-nitroanilide + H2O
N-acetyl-L-Phe-Gly + 4-nitroaniline
Ficus sp.
-
the index of stereochemical selectivity for N-acetyl-L-Phe-Gly 4-nitroanilide and N-acetyl-D-Phe-Gly 4-nitroanilide is 93
-
-
?
N-benzoyl-DL-arginine-2-naphthylamide + H2O
N-benzoyl-DL-arginine + 2-naphthylamine
Ficus sp.
-
-
-
-
?
N-benzyloxycarbonyl-D-Ala p-nitrophenyl ester + H2O
N-benzyloxycarbonyl-D-Ala + 4-nitrophenol
Ficus sp.
-
ficin II
-
-
?
N-benzyloxycarbonyl-D-Leu p-nitrophenyl ester + H2O
N-benzyloxycarbonyl-D-Leu + 4-nitrophenol
Ficus sp.
-
ficin II
-
-
?
N-benzyloxycarbonyl-D-Phe p-nitrophenyl ester + H2O
N-benzyloxycarbonyl-D-Phe + 4-nitrophenol
Ficus sp.
-
ficin II
-
-
?
N-benzyloxycarbonyl-D-Tyr p-nitrophenyl ester + H2O
N-benzyloxycarbonyl-D-Tyr + 4-nitrophenol
Ficus sp.
-
ficin II
-
-
?
N-tert-butyloxycarbonyl-Ala-(1-4)-guanidinonaphthyl ester + L-Ala-p-nitroanilide
N-tert-butyloxycarbonyl-Ala-Ala-p-nitroanilide + ?
Ficus sp.
-
-
-
-
?
N-tert-butyloxycarbonyl-Ala-(1-5)-guanidinonaphthyl ester + L-Ala-p-nitroanilide
N-tert-butyloxycarbonyl-Ala-Ala-p-nitroanilide + ?
Ficus sp.
-
-
-
-
?
N-tert-butyloxycarbonyl-Ala-(1-6)-guanidinonaphthyl ester + L-Ala-p-nitroanilide
N-tert-butyloxycarbonyl-Ala-Ala-p-nitroanilide + ?
Ficus sp.
-
-
-
-
?
N-tert-butyloxycarbonyl-Ala-(2-5)-guanidinonaphthyl ester + L-Ala-p-nitroanilide
N-tert-butyloxycarbonyl-Ala-Ala-p-nitroanilide + ?
Ficus sp.
-
-
-
-
?
N-tert-butyloxycarbonyl-Ala-m-(guanidinomethyl)phenyl ester + L-Ala-p-nitroanilide
N-tert-butyloxycarbonyl-Ala-Ala-p-nitroanilide + ?
Ficus sp.
-
-
-
-
?
N-tert-butyloxycarbonyl-Ala-m-guanidinophenyl ester + L-Ala-p-nitroanilide
N-tert-butyloxycarbonyl-Ala-Ala-p-nitroanilide + ?
Ficus sp.
-
-
-
-
?
N-tert-butyloxycarbonyl-Ala-p-(guanidinomethyl)phenyl ester + L-Ala-p-nitroanilide
N-tert-butyloxycarbonyl-Ala-Ala-p-nitroanilide + ?
Ficus sp.
-
-
-
-
?
N-tert-butyloxycarbonyl-Ala-Ph + L-Ala-p-nitroanilide
N-tert-butyloxycarbonyl-Ala-Ala-p-nitroanilide + phenol
Ficus sp.
-
-
-
-
?
N-tert-butyloxycarbonyl-L-Ala-p-amidinophenyl ester + L-Ala-p-nitroanilide
N-tert-butyloxycarbonyl-L-Ala-L-Ala-p-nitroanilide + ?
Ficus sp.
-
-
-
-
?
N-tert-butyloxycarbonyl-L-Ala-p-guanidinophenyl ester + L-Ala-p-nitroanilide
N-tert-butyloxycarbony-L-Ala-L-Ala-p-nitroanilide + ?
Ficus sp.
-
-
-
-
?
Nalpha-benzoyl-DL-arginine 4-nitroanilide + H2O
Nalpha-benzoyl-DL-arginine + 4-nitroaniline
-
-
-
-
?
Nalpha-benzoyl-DL-arginine-4-nitroanilide + H2O
Nalpha-benzoyl-DL-arginine + 4-nitroaniline
-
-
-
-
?
Nalpha-carbobenzoxy-Ala p-nitrophenyl ester + H2O
Nalpha-carbobenzoxy-Ala + 4-nitrophenol
Ficus sp.
-
ficin II
-
-
?
Nalpha-carbobenzoxy-Leu p-nitrophenyl ester + H2O
Nalpha-carbobenzoxy-Leu + 4-nitrophenol
Ficus sp.
-
ficin II
-
-
?
Nalpha-carbobenzoxy-Lys p-nitrophenyl ester + H2O
Nalpha-carbobenzoxy-Lys + 4-nitrophenyl
Ficus sp.
-
ficin II
-
-
?
Nalpha-carbobenzoxy-Phe p-nitrophenyl ester + H2O
Nalpha-carbobenzoxy-Phe + 4-nitrophenol
Ficus sp.
-
ficin II
-
-
?
Nalpha-carbobenzoxy-Tyr p-nitrophenyl ester + H2O
Nalpha-carbobenzoxy-Tyr + 4-nitrophenol
Ficus sp.
-
ficin II
-
-
?
Nalpha-carbobenzoxy-Val p-nitrophenyl ester + H2O
Nalpha-carbobenzoxy-Val + 4-nitrophenyl
Ficus sp.
-
ficin II
-
-
?
Nalpha-DL-arginine 4-nitroanilide + H2O
Nalpha-L-arginine + 4-nitroaniline
-
-
-
-
?
oxan-2-one + 4-bromobenzaldehyde
?
-
reaction condition: 30°C, MeCN (0.85 mL) and deionized water (0.15 mL). Yield: 29%, 77:23 anti/syn ratio, enantioselectivity: 71%/27% (anti/syn)
-
-
?
oxan-2-one + 4-nitrobenzaldehyde
?
-
reaction condition: 30°C, MeCN (0.85 mL) and deionized water (0.15 mL). Yield: 21%, 42:58 anti/syn ratio, enantioselectivity: 60%/24% (anti/syn)
-
-
?
oxan-3-one + 4-nitrobenzaldehyde
?
-
reaction condition: 30°C, MeCN (0.85 mL) and deionized water (0.15 mL). Yield: 28%, 54:46 anti/syn ratio, enantioselectivity: 57%/28% (anti/syn)
-
-
?
oxan-4-one + 4-nitrobenzaldehyde
?
-
reaction condition: 30°C, MeCN (0.85 mL) and deionized water (0.15 mL). Yield: 31%, 53:47 anti/syn ratio, enantioselectivity: 25%/17% (anti/syn)
-
-
?
p-nitrophenyl N-acetylglycinate + H2O
p-nitrophenol + N-acetylglycine
Ficus sp.
-
ficin II and III
-
-
?
p-nitrophenyl N-benzoylglycinate + H2O
p-nitrophenol + N-benzoylglycine
Ficus sp.
-
ficin II and III
-
-
?
p-nitrophenyl N-benzyloxycarbonylglycinate + H2O
p-nitrophenol + N-benzyloxycarbonylglycine
Ficus sp.
-
ficin II and III
-
-
?
p-nitrophenyl N-formylglycinate + H2O
p-nitrophenol + N-formylglycine
Ficus sp.
-
ficin II and III
-
-
?
p-nitrophenyl N-trans-cinnamoylglycinate + H2O
p-nitrophenol + N-trans-cinnamoylglycine
Ficus sp.
-
ficin II and III
-
-
?
Phe-citrulline-p-nitroanilide + H2O
?
Ficus sp.
-
activity is 3.8fold higher than with benzoyl-DL-Arg-p-nitroanilide
-
-
?
tert-butyl 4-oxopiperidine-1-carboxylate + 3-bromobenzaldehyde
?
-
reaction condition: 30°C, MeCN (0.85 mL) and deionized water (0.15 mL). Yield: 25%, 47:53 anti/syn ratio, enantioselectivity: 51%/45% (anti/syn)
-
-
?
tert-butyl 4-oxopiperidine-1-carboxylate + 3-nitrobenzaldehyde
?
-
reaction condition: 30°C, MeCN (0.85 mL) and deionized water (0.15 mL). Yield: 29%, 51:49 anti/syn ratio, enantioselectivity: 59%/45% (anti/syn)
-
-
?
tert-butyl 4-oxopiperidine-1-carboxylate + 4-bromobenzaldehyde
?
-
reaction condition: 30°C, MeCN (0.85 mL) and deionized water (0.15 mL). Yield: 35%, 75:25 anti/syn ratio, enantioselectivity: 67%/36% (anti/syn)
-
-
?
tert-butyl 4-oxopiperidine-1-carboxylate + 4-nitrobenzaldehyde
tert-butyl 3-[hydroxy(4-nitrophenyl)methyl]-4-oxopiperidine-1-carboxylate
tert-butyloxycarbonyl-L-Ala-L-Ala-Gly-4-nitroanilide + H2O
tert-butyloxycarbonyl-L-Ala-L-Ala-Gly + 4-nitroaniline
-
-
-
-
?
thian-4-one + 3-nitrobenzaldehyde
?
-
reaction condition: 30°C, MeCN (0.85 mL) and deionized water (0.15 mL). Yield: 26%, 86:14 anti/syn ratio, enantioselectivity: 77%/1% (anti/syn)
-
-
?
thian-4-one + 4-cyanobenzaldehyde
?
-
reaction condition: 30°C, MeCN (0.85 mL) and deionized water (0.15 mL). Yield: 23%, 84:16 anti/syn ratio, enantioselectivity: 76%/53% (anti/syn)
-
-
?
thiolan-3-one + 4-nitrobenzaldehyde
?
-
reaction condition: 30°C, MeCN (0.85 mL) and deionized water (0.15 mL). Yield: 31%, 40:60 anti/syn ratio, enantioselectivity: 45%/23% (anti/syn)
-
-
?
Nalpha-Benzoyl-L-Arg ethyl ester + H2O
Nalpha-Benzoyl-L-Arg + ethanol
-
-
-
-
?
Nalpha-Benzoyl-L-Arg ethyl ester + H2O
Nalpha-Benzoyl-L-Arg + ethanol
Ficus sp.
-
-
-
-
?
Nalpha-carbobenzoxy-Gly p-nitrophenyl ester + H2O
Nalpha-carbobenzoxy-Gly + 4-nitrophenol
Ficus sp.
-
-
-
-
?
Nalpha-carbobenzoxy-Gly p-nitrophenyl ester + H2O
Nalpha-carbobenzoxy-Gly + 4-nitrophenol
Ficus sp.
-
ficin II
-
-
?
tert-butyl 4-oxopiperidine-1-carboxylate + 4-nitrobenzaldehyde
tert-butyl 3-[hydroxy(4-nitrophenyl)methyl]-4-oxopiperidine-1-carboxylate
-
reaction condition: 30°C, MeCN (0.85 mL) and deionized water (0.15 mL). Yield: 36%, 41:59 anti/syn ratio, enantioselectivity: 60%/14% (anti/syn)
-
-
?
tert-butyl 4-oxopiperidine-1-carboxylate + 4-nitrobenzaldehyde
tert-butyl 3-[hydroxy(4-nitrophenyl)methyl]-4-oxopiperidine-1-carboxylate
-
the catalytic activity and stereoselectivity of ficin are significantly influenced by reaction media. Best yield of 34% with a poor enantioselectivity of 29% in THF. Best enantioselectivity of 57% with a yield of 28% in MeCN. In tested polar solvents including i-PrOH, DMSO, and DMF, ficin shows lower activityand enantioselectivity
-
-
?
tert-butyl 4-oxopiperidine-1-carboxylate + 4-nitrobenzaldehyde
tert-butyl 3-[hydroxy(4-nitrophenyl)methyl]-4-oxopiperidine-1-carboxylate
-
water content is important for enantioselectivity and enzymatic activity. When water content is at 0.15 (H2O/(H2O+MeCN): 0.15,v/v) the reaction give the best enantioselectivity of 59% with 32% yield
-
-
?
additional information
?
-
-
destruction of Staphylococcus aureus and Staphylococcus epidermidis biofilms with ficin
-
-
?
additional information
?
-
-
a wide variety of peptide bonds is hydrolyzed, but peptide bonds following an aromatic residue appear to be hydrolyzed more efficiently than the other
-
-
?
additional information
?
-
-
ficain is the major proteolytic activity component in the milky latex of the plant
-
-
?
additional information
?
-
-
substrate specificity compared to papain, the ficain S1-pocket accepts Gly, SEr, Glu, Tyr, and Phe, the S2-pocket accepts cysteic acid, Val, Leu, Gly, and Phe, Arg-NH-nitrophenyl is a poor substrate
-
-
?
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NATURAL SUBSTRATE
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate)
(Substrate)
LITERATURE
(Substrate)
(Substrate)
COMMENTARY
(Product)
(Product)
LITERATURE
(Product)
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
r=reversible
ir=irreversible
?=not specified
additional information
?
-
-
ficain is the major proteolytic activity component in the milky latex of the plant
-
-
?
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METALS and IONS
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
additional information
-
ficin E is not affected by Mg2+, Ca2+ and Mn2+ at a concentration up to 10 mM
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INHIBITOR
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
amaranth cystatin
Ficus sp.
-
Amaranthus hypochondracus cysteine proteinase inhibitor, GenBank accession number DQ792503
-
methylmethanethiol sulfonate
-
complete inhibition of all five ficin isoforms at 3 mM
Nalpha-tosyl-L-lysine chloromethyl ketone
-
complete inhibition of all five ficin isoforms at 0.1 mM
Nalpha-tosyl-L-phenylalanine chloromethylketone
-
complete inhibition of all five ficin isoforms at 0.1 mM
p-chloromercuribenzoate
-
activity is completely inhibited by the cysteine-group specific inhibitor
Sodium tetrathionate
-
activity is completely inhibited by the cysteine-group specific inhibitor
iodoacetamide
-
activity is completely inhibited, cysteine-group specific inhibitor, confirms the participation of the cysteine residue at the active site of the enzyme
Proflavine
Ficus sp.
-
the inhibitor increases the noncovalent reversible binding affinity of ficin towards the maleimides without affecting the nucleophilic reactivity of the catalytically essential thiol groups which are alkylated in the inactivation reaction
additional information
-
all five ficin isoforms are not inhibited by pepstatin A, Pafabloc SC, and EDTA
-
additional information
-
ficin E is not affected by Mg2+, Ca2+ and Mn2+ at a concentration up to 10 mM. It is unaffected by synthetic (Pefabloc SC, benzamidine) and by natural proteinaceous (aprotinin) serine proteases inhibitors, by aspartic proteases inhibitors (pepstatin A) and by metallo-proteases inhibitors (EDTA, EGTA)
-
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ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
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DISEASE
TITLE OF PUBLICATION
LINK TO PUBMED
Arteriovenous Fistula
The effect of a distal arteriovenous fistula on the patency of ficin digested bovine carotid arteries bypassing the infra-renal vena cava in dogs.
Breast Neoplasms
Immunocytochemical assay for estrogen receptor with monoclonal antibody D753P gamma in routinely processed formaldehyde-fixed breast tissue. Comparison with frozen section assay and with monoclonal antibody H222.
Carcinoma
The use of proteolysis with ficin, for immunostaining of paraffin sections. A study of lymphoid, mesenchymal, and epithelial determinants in human tissues.
Fabry Disease
Correction of the enzymic defect in cultured fibroblasts from patients with Fabry's disease: treatment with purified alpha-galactosidase from ficin.
Fabry Disease
Enzyme replacement in Fabry disease: treatment of cultured skin fibroblasts with a purified alpha-galactosidase from ficin.
Hepatitis A
Effect of Plant-Derived Proteases on Infectivity of Tulane Virus, Murine Norovirus, and Hepatitis A Virus.
Hypersensitivity
Sensitization to Ficus benjamina: relationship to natural rubber latex allergy and identification of foods implicated in the Ficus-fruit syndrome.
Lung Neoplasms
[Preparation of McAb against lung cancer and its application in tumor radioimmunoimaging]
Melanoma, Experimental
Purification and characterization of a low molecular mass cysteine proteinase inhibitor from human amniotic fluid.
Neoplasms
Efficacy of different proteolytic treatments for the immunohistochemical stain of cytokeratins.
Neoplasms
The use of proteolysis with ficin, for immunostaining of paraffin sections. A study of lymphoid, mesenchymal, and epithelial determinants in human tissues.
Nerve Sheath Neoplasms
The use of proteolysis with ficin, for immunostaining of paraffin sections. A study of lymphoid, mesenchymal, and epithelial determinants in human tissues.
Prostatic Neoplasms
Biotechnological Potential of Araucaria angustifolia Pine Nuts Extract and the Cysteine Protease Inhibitor AaCI-2S.
Purpura, Thrombocytopenic, Idiopathic
Use of enzyme treatment to enhance reactivity of HLA and platelet-specific antibodies in solid-phase RBC adherence assays.
Sarcoma
The use of proteolysis with ficin, for immunostaining of paraffin sections. A study of lymphoid, mesenchymal, and epithelial determinants in human tissues.
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KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
0.0172
Nalpha-carbobenzoxy-glycylglycine p-nitrophenyl ester
Ficus sp.
-
pH 6.36, 23.2% v/v CH3CN, ficin II
0.0377
Nalpha-carbobenzoxy-Phe p-nitrophenyl ester
Ficus sp.
-
pH 6.36, 23.2% v/v CH3CN, ficin II
0.0034
Nalpha-carbobenzoxy-Tyr p-nitrophenyl ester
Ficus sp.
-
pH 6.36, 23.2% v/v CH3CN, ficin II
0.0327
Nalpha-carbobenzoxy-Val p-nitrophenyl ester
Ficus sp.
-
pH 6.36, 23.2% v/v CH3CN, ficin II
0.0059
Nalpha-carbobenzoxy-Ala p-nitrophenyl ester
Ficus sp.
-
pH 6.0, 3.17% v/v CH3CN, ficin II
0.0122
Nalpha-carbobenzoxy-Ala p-nitrophenyl ester
Ficus sp.
-
pH 6.36, 23.2% v/v CH3CN, ficin II
0.0258
Nalpha-carbobenzoxy-Ala p-nitrophenyl ester
Ficus sp.
-
pH 6.36, 23.2% v/v CH3CN, ficin II
0.0134
Nalpha-carbobenzoxy-Gly p-nitrophenyl ester
Ficus sp.
-
pH 6.0, 3.17% v/v CH3CN, ficin II
0.0598
Nalpha-carbobenzoxy-Gly p-nitrophenyl ester
Ficus sp.
-
pH 6.36, 23.2% v/v CH3CN, ficin II
0.0087
Nalpha-carbobenzoxy-Lys p-nitrophenyl ester
Ficus sp.
-
pH 6.0, 3.17% v/v CH3CN, ficin II
0.052
Nalpha-carbobenzoxy-Lys p-nitrophenyl ester
Ficus sp.
-
pH 6.36, 23.2% v/v CH3CN, ficin II
additional information
additional information
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little or no effect of temperature on Km
-
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