EC Number   |
|---|
   3.4.21.53 | - |
| 3.4.21.53 | 139fold, ion exchange chromatography and gel filtration |
| 3.4.21.53 | active lon carrying six C-terminal histidine residues is overexpressed in yeast, is rapidly purified to homogeneity from an isolated mitochondrial extract on a Ni21-NTA column, and purified to homogeneity by gel filtration on a Superose 6 column |
| 3.4.21.53 | by gel filtration, to near homogeneity |
| 3.4.21.53 | by successive Ni2+-NTA affinity chromatography and anion-exchange chromatography |
| 3.4.21.53 | gel filtration |
| 3.4.21.53 | human lon is overproduced and purified from the Rosetta Escherichia coli strain. Human mitochondrial lon degrades mouse StAR in the presence of ATP, but not in its absence |
| 3.4.21.53 | lon-N119 purified. Digestion of lonA by alpha-chymotrypsin yields a stable fragment consisting of residues 491-584 purified. lon proteolytic domain purified |
| 3.4.21.53 | lonR9 product |
| 3.4.21.53 | mutants purified to homogeneity |
