EC Number   |
|---|
    2.9.1.1 | - |
| 2.9.1.1 | ammonium sulfate precipitation, hydroxyapatite column chromatography, Hi Trap Q column chromatography, and Superdex 200 gel filtration |
| 2.9.1.1 | centrifugation at 150000 xg, followed by succesive chromatography of the supernatant on DEAE-cellulose, Sephacryl S-300 and Heparin-Toyopearl |
| 2.9.1.1 | recombinant C-terminally His6-tagged SecS from Escherichia coli strain Rosetta2(DE3) by nickel affinity chromatography and gel filtration to over 95% purity |
| 2.9.1.1 | recombinant SelA from Escherichia coli strain DH5alpha by anion exchange chromatography, ammonium sulfate fractionation, and gel filtration |
