Any feedback?
Information on EC 2.9.1.1 - L-seryl-tRNASec selenium transferase
for references in articles please use BRENDA:EC2.9.1.1Please wait a moment until all data is loaded. This message will disappear when all data is loaded.
EC Tree
2.9.1.1 L-seryl-tRNASec selenium transferaseIUBMB Comments
A pyridoxal 5'-phosphate enzyme identified in Escherichia coli. Recognises specifically tRNASec-species. Binding of tRNASec also occurs in the absence of the seryl group. 2-Aminoacryloyl-tRNA, bound to the enzyme as an imine with the pyridoxal phosphate, is an intermediate in the reaction. Since the selenium atom replaces oxygen in serine, the product may also be referred to as L-selenoseryl-tRNASec. The symbol Sel has also been used for selenocysteine but Sec is preferred.
Specify your search results
Word Map
- 2.9.1.1
- autoantibodies
-
selenoproteins
-
antinuclear
-
anti-sla
-
antimitochondrial
- sepsecs
-
selenocysteyl-trnasec
-
secis
-
trnasersec
-
anti-liver
-
selenocysteine-containing
- trnaser
-
anti-lkm1
-
liver-pancreas
-
anti-smooth
-
ser-trnasec
-
non-organ
-
selenoproteome
The enzyme appears in viruses and cellular organisms
Synonyms
soluble liver antigen, selenocysteine synthase, sec synthase, selenocysteinyl-trna synthase, 
more
topPlease wait a moment until the data is sorted. This message will disappear when the data is sorted.
SYNONYM 
ORGANISM
UNIPROT
COMMENTARY 
LITERATURE
Cysteinyl-tRNA(Sec) selenium transferase
-
-
-
-
Cysteinyl-tRNASec-selenium transferase
-
-
-
-
CysteinyltRNASel-selenium transferase
-
-
-
-
GenBank Y14814-derived protein GI 2440135
-
-
-
-
L-Selenocysteine-tRNASec synthase selenocysteine synthase
-
-
-
-
L-Selenocysteine-tRNASel synthase
-
-
-
-
Sec synthase
SecS
SelA
Selenocysteine synthase
Selenocysteine synthase (Moorella thermoacetica strain DSM521 clone pCTA 100/pCTAB1 gene selA)
-
-
-
-
Selenocysteine-tRNA synthase
-
-
-
-
Selenocysteinyl-tRNA synthase
Selenocysteinyl-tRNA(Sec) synthase
-
-
-
-
SLA
soluble liver antigen
Synthase, selenocysteine (Moorella thermoacetica clone pCTA100/pCTAB1 gene selA)
-
-
-
-
Synthase, selenocysteinyl-transfer ribonucleate
-
-
-
-
additional information
-
SecS is a member of the fold type I family of PLP-dependent enzymes with distinct structural elements
Selenocysteine synthase
-
-
-
-
Selenocysteinyl-tRNA synthase
-
-
-
-
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
REACTION
REACTION DIAGRAM
COMMENTARY
ORGANISM
UNIPROT
LITERATURE
L-seryl-tRNASec + selenophosphate = L-selenocysteinyl-tRNASec + phosphate
the formyl group of pyridoxal phosphate forms a Schiff base with the alpha-amino group of L-Ser with the subsequent 2,3-elimination of a water molecule and the generation of an aminoacrylyl-tRNASecUCA intermediate
-
L-seryl-tRNASec + selenophosphate = L-selenocysteinyl-tRNASec + phosphate
tRNA is bound near the margin of the dimeric subunits, appears to bind only one seryl-tRNA Sec per dimer
-
L-seryl-tRNASec + selenophosphate = L-selenocysteinyl-tRNASec + phosphate
substrate binding and reaction mechanism
-
L-seryl-tRNASec + selenophosphate = L-selenocysteinyl-tRNASec + phosphate
-
-
-
-
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
Selenium transfer
Selenium transfer
-
-
-
-
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
PATHWAY SOURCE
PATHWAYS
BRENDA
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
SYSTEMATIC NAME
IUBMB Comments
selenophosphate:L-seryl-tRNASec selenium transferase
A pyridoxal 5'-phosphate enzyme identified in Escherichia coli. Recognises specifically tRNASec-species. Binding of tRNASec also occurs in the absence of the seryl group. 2-Aminoacryloyl-tRNA, bound to the enzyme as an imine with the pyridoxal phosphate, is an intermediate in the reaction. Since the selenium atom replaces oxygen in serine, the product may also be referred to as L-selenoseryl-tRNASec. The symbol Sel has also been used for selenocysteine but Sec is preferred.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
CAS REGISTRY NUMBER
COMMENTARY
183869-06-9
-
211752-49-7
synthase, selenocysteine (Moorella thermoacetica clone pCTA100/pCTAB1 gene selA) /GenBank Y14814-derived protein GI 2440135 /selenocysteine synthase (Moorella thermoacetica strain DSM521 clone pCTA 100/pCTAB1 gene selA)
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
SUBSTRATE
PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate)
(Substrate)
LITERATURE
(Substrate)
(Substrate)
COMMENTARY
(Product)
(Product)
LITERATURE
(Product)
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
r=reversible
ir=irreversible
?=not specified
L-Seryl-tRNASec + selenide
?
-
active only at much higher concentrations than those of the selenophosphate
-
-
?
L-Seryl-tRNASec + selenophosphate
?
-
the enzyme is involved in the biosynthesis of selenocysteine
-
-
?
L-Seryl-tRNASec + sulfide
?
-
active only at much higher concentrations than those of the thiophosphate
-
-
?
L-Seryl-tRNASec + thiophosphate
Cysteyl-tRNASec + H2O + phosphate
-
-
-
?
seryl-tRNASec + monoselenophosphate
selenocysteinyl-tRNASec + H2O + phosphate
-
selenoamino acid metabolism
-
-
?
L-Seryl-tRNASec + selenophosphate
L-Selenocysteinyl-tRNASec + H2O + phosphate
-
-
-
?
L-Seryl-tRNASec + selenophosphate
L-Selenocysteinyl-tRNASec + H2O + phosphate
-
-
-
?
L-Seryl-tRNASec + selenophosphate
L-Selenocysteinyl-tRNASec + H2O + phosphate
-
-
-
?
L-Seryl-tRNASec + selenophosphate
L-Selenocysteinyl-tRNASec + H2O + phosphate
-
-
-
?
L-Seryl-tRNASec + selenophosphate
L-Selenocysteinyl-tRNASec + H2O + phosphate
-
-
-
?
L-Seryl-tRNASec + selenophosphate
L-Selenocysteinyl-tRNASec + H2O + phosphate
-
-
-
?
L-Seryl-tRNASec + selenophosphate
L-Selenocysteinyl-tRNASec + H2O + phosphate
-
-
-
?
L-Seryl-tRNASec + selenophosphate
L-Selenocysteinyl-tRNASec + H2O + phosphate
-
-
-
?
L-Seryl-tRNASec + selenophosphate
L-Selenocysteinyl-tRNASec + H2O + phosphate
-
-
-
?
L-seryl-tRNASec + selenophosphate
L-selenocysteinyl-tRNASec + phosphate
-
-
-
?
L-seryl-tRNASec + selenophosphate
L-selenocysteinyl-tRNASec + phosphate
-
-
-
-
?
L-seryl-tRNASec + selenophosphate
L-selenocysteinyl-tRNASec + phosphate
-
-
-
-
?
L-seryl-tRNASec + selenophosphate
L-selenocysteinyl-tRNASec + phosphate
-
sps2 encodes a selenoprotein, SPS2, which can serve as selenium donor for the biosynthesis of Sec, as an autoregulator of selenoprotein synthesis, Sec biosynthetic pathway in eukaryotes, overview
-
-
?
L-seryl-tRNASec + selenophosphate
L-selenocysteinyl-tRNASec + phosphate
-
-
-
-
?
L-seryl-tRNASec + selenophosphate
L-selenocysteinyl-tRNASec + phosphate
-
-
-
-
?
L-seryl-tRNASec + selenophosphate
L-selenocysteinyl-tRNASec + phosphate
-
-
-
-
?
L-seryl-tRNASec + selenophosphate
L-selenocysteinyl-tRNASec + phosphate
-
the enzyme is involved in selenocysteine incorporation into proteins in bacteria, pathway overview
-
-
?
L-seryl-tRNASec + selenophosphate
L-selenocysteinyl-tRNASec + phosphate
-
-
-
-
?
L-seryl-tRNASec + selenophosphate
L-selenocysteinyl-tRNASec + phosphate
-
sps2 encodes a selenoprotein, SPS2, which can serve as selenium donor for the biosynthesis of Sec, as an autoregulator of selenoprotein synthesis, Sec biosynthetic pathway in eukaryotes, overview
-
-
?
L-seryl-tRNASec + selenophosphate
L-selenocysteinyl-tRNASec + phosphate
-
the efficiency of tRNA binding in descending order is O-phosphoseryl-tRNA[Ser]Sec, tRNA[Ser]Sec, and seryl-tRNA[Ser]Sec, whereas seryl-tRNASer and tRNASer do not bind at all
-
-
?
L-seryl-tRNASec + selenophosphate
L-selenocysteinyl-tRNASec + phosphate
-
the phosphate loop accommodates the gamma-phosphate moiety of O-phospho-L-seryltRNA [Ser]Sec and, after phosphate elimination, binds selenophosphate to initiate attack on the proposed aminoacrylyltRNA [Ser]Sec intermediate. Binding of phosphate triggers disorder-order transition in an active site loop
-
-
?
seryl-tRNASec + selenophosphate
selenocysteinyl-tRNASec + H2O + phosphate
-
-
-
-
?
seryl-tRNASec + selenophosphate
selenocysteinyl-tRNASec + H2O + phosphate
-
selenoamino acid metabolism
-
-
?
additional information
?
-
-
SecS occurs in patients with autoimmune chronic hepatitis as an autoimmune factor that co-precipitated tRNA[Ser]Sec in cell extracts of these individuals andis known as the SLA, soluble liver antigen
-
-
?
additional information
?
-
-
SelA interacts with the transcription factor SelB, mutational interaction study, overview
-
-
?
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
NATURAL SUBSTRATE
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate)
(Substrate)
LITERATURE
(Substrate)
(Substrate)
COMMENTARY
(Product)
(Product)
LITERATURE
(Product)
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
r=reversible
ir=irreversible
?=not specified
L-Seryl-tRNASec + selenophosphate
?
-
the enzyme is involved in the biosynthesis of selenocysteine
-
-
?
seryl-tRNASec + monoselenophosphate
selenocysteinyl-tRNASec + H2O + phosphate
-
selenoamino acid metabolism
-
-
?
seryl-tRNASec + selenophosphate
selenocysteinyl-tRNASec + H2O + phosphate
-
selenoamino acid metabolism
-
-
?
L-seryl-tRNASec + selenophosphate
L-selenocysteinyl-tRNASec + phosphate
-
-
-
?
L-seryl-tRNASec + selenophosphate
L-selenocysteinyl-tRNASec + phosphate
-
-
-
-
?
L-seryl-tRNASec + selenophosphate
L-selenocysteinyl-tRNASec + phosphate
-
sps2 encodes a selenoprotein, SPS2, which can serve as selenium donor for the biosynthesis of Sec, as an autoregulator of selenoprotein synthesis, Sec biosynthetic pathway in eukaryotes, overview
-
-
?
L-seryl-tRNASec + selenophosphate
L-selenocysteinyl-tRNASec + phosphate
-
-
-
-
?
L-seryl-tRNASec + selenophosphate
L-selenocysteinyl-tRNASec + phosphate
-
-
-
-
?
L-seryl-tRNASec + selenophosphate
L-selenocysteinyl-tRNASec + phosphate
-
the enzyme is involved in selenocysteine incorporation into proteins in bacteria, pathway overview
-
-
?
L-seryl-tRNASec + selenophosphate
L-selenocysteinyl-tRNASec + phosphate
-
-
-
-
?
L-seryl-tRNASec + selenophosphate
L-selenocysteinyl-tRNASec + phosphate
-
sps2 encodes a selenoprotein, SPS2, which can serve as selenium donor for the biosynthesis of Sec, as an autoregulator of selenoprotein synthesis, Sec biosynthetic pathway in eukaryotes, overview
-
-
?
additional information
?
-
-
SecS occurs in patients with autoimmune chronic hepatitis as an autoimmune factor that co-precipitated tRNA[Ser]Sec in cell extracts of these individuals andis known as the SLA, soluble liver antigen
-
-
?
additional information
?
-
-
SelA interacts with the transcription factor SelB, mutational interaction study, overview
-
-
?
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
COFACTOR
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
pyridoxal 5'-phosphate
-
contains 1 mol of bound pyridoxal 5-phosphate per mol of 50000 MW subunit
pyridoxal 5'-phosphate
-
SecS is a a PLP-dependent transferase. The PLP cofactor iIs tightly anchored by non-canonical contacts to both protomers of a close dimer
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
DISEASE
TITLE OF PUBLICATION
LINK TO PUBMED
Hepatitis
Antibodies to soluble liver antigen: an additional marker in type 1 auto-immune hepatitis.
Hepatitis
Autoimmune hepatitis-specific antibodies against soluble liver antigen and liver cytosol type 1 in patients with chronic viral hepatitis.
Hepatitis
Characterisation of a new subgroup of autoimmune chronic active hepatitis by autoantibodies against a soluble liver antigen.
Hepatitis
Detection of autoantibodies against M2, LKM-1, and SLA in liver diseases by standardized uniform ELISA-techniques.
Hepatitis
Identification of T cell epitopes on soluble liver antigen in Chinese patients with auto-immune hepatitis.
Hepatitis
Significance of antibodies to soluble liver antigen/liver pancreas: a large French study.
Hepatitis
[A retrospective study on the role of antibodies against soluble liver antigen (anti-SLA antibodies) and other autoantibodies in the diagnostics of autoimmune hepatitis]
Hepatitis A
False-positive serologies for acute hepatitis A and autoimmune hepatitis in a patient with acute Epstein-Barr virus infection.
Hepatitis A
Prognostic Implications of Antibodies to Soluble Liver Antigen in Autoimmune Hepatitis: A PRISMA-Compliant Meta-Analysis.
Hepatitis, Autoimmune
Antibodies to conformational epitopes of soluble liver antigen define a severe form of autoimmune liver disease.
Hepatitis, Autoimmune
Antibodies to soluble liver antigen and alpha-enolase in patients with autoimmune hepatitis.
Hepatitis, Autoimmune
Antibodies to soluble liver antigen in patients with various liver diseases: a multicentre study.
Hepatitis, Autoimmune
Antibodies to soluble liver antigen, P450IID6, and mitochondrial complexes in chronic hepatitis.
Hepatitis, Autoimmune
Antibodies to soluble liver antigen: specific marker of autoimmune hepatitis.
Hepatitis, Autoimmune
Clinical significance of autoantibodies to soluble liver antigen in autoimmune hepatitis.
Hepatitis, Autoimmune
Development of a recombinant cell-based indirect immunofluorescence assay for the determination of autoantibodies against soluble liver antigen in autoimmune hepatitis.
Hepatitis, Autoimmune
Discrimination of autoimmune hepatitis: autoantibody typing and beyond.
Hepatitis, Autoimmune
False-positive serologies for acute hepatitis A and autoimmune hepatitis in a patient with acute Epstein-Barr virus infection.
Hepatitis, Autoimmune
Fine specificity of autoantibodies to soluble liver antigen and liver/pancreas.
Hepatitis, Autoimmune
Identification of target antigen for SLA/LP autoantibodies in autoimmune hepatitis.
Hepatitis, Autoimmune
Immunoreactivity to cytokeratin 8/18 in patients with soluble liver antigen (SLA) positive autoimmune hepatitis (AIH type 3) is not sufficient for diagnostic use.
Hepatitis, Autoimmune
Isolation and characterization of cDNA encoding the antigenic protein of the human tRNP(Ser)Sec complex recognized by autoantibodies from patients withtype-1 autoimmune hepatitis.
Hepatitis, Autoimmune
Prognostic implications of antibodies to Ro/SSA and soluble liver antigen in type 1 autoimmune hepatitis.
Hepatitis, Autoimmune
Prognostic Implications of Antibodies to Soluble Liver Antigen in Autoimmune Hepatitis: A PRISMA-Compliant Meta-Analysis.
Hepatitis, Autoimmune
Soluble liver antigen: isolation of a 35-kd recombinant protein (SLA-p35) specifically recognizing sera from patients with autoimmune hepatitis.
Hepatitis, Autoimmune
Study of nonstandard auto-antibodies as prognostic markers in auto immune hepatitis in children.
Hepatitis, Autoimmune
Targets of antibodies to soluble liver antigen in patients with autoimmune hepatitis.
Hepatitis, Autoimmune
[A retrospective study on the role of antibodies against soluble liver antigen (anti-SLA antibodies) and other autoantibodies in the diagnostics of autoimmune hepatitis]
Hepatitis, Autoimmune
[The clinical laboratory in the diagnosis of autoimmune liver diseases]
Hepatitis, Chronic
Antibodies to soluble liver antigen, P450IID6, and mitochondrial complexes in chronic hepatitis.
Hepatitis, Chronic
Characterisation of a new subgroup of autoimmune chronic active hepatitis by autoantibodies against a soluble liver antigen.
Hepatitis, Chronic
Detection of autoantibodies against M2, LKM-1, and SLA in liver diseases by standardized uniform ELISA-techniques.
Liver Cirrhosis, Biliary
Detection of autoantibodies against M2, LKM-1, and SLA in liver diseases by standardized uniform ELISA-techniques.
Liver Cirrhosis, Biliary
Treatment of chronic type B hepatitis with recombinant alpha-interferon induces autoantibodies not specific for autoimmune chronic hepatitis.
Liver Diseases
Antibodies to conformational epitopes of soluble liver antigen define a severe form of autoimmune liver disease.
Liver Diseases
Antibodies to soluble liver antigen in patients with various liver diseases: a multicentre study.
Liver Diseases
Antibodies to soluble liver antigen, P450IID6, and mitochondrial complexes in chronic hepatitis.
Liver Diseases
Treatment of chronic type B hepatitis with recombinant alpha-interferon induces autoantibodies not specific for autoimmune chronic hepatitis.
Nervous System Diseases
Selenium homeostasis and antioxidant selenoproteins in brain: Implications for disorders in the central nervous system.
Nervous System Diseases
Structural basis for early-onset neurological disorders caused by mutations in human selenocysteine synthase.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
additional information
additional information
-
kinetics study of selenocysteine incorporation
-
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
pI VALUE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
ORGANISM
COMMENTARY
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
SOURCE
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY
GeneOntology No.
LITERATURE
SOURCE
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
physiological function
physiological function
-
the enzyme is dispensable for the parasite survival
physiological function
-
the enzyme is dispensable for the parasite survival
-
Show AA Sequence and Transmembrane Helices (12464 entries)
Longer loading times are possible. Please use the AA Sequence and Transmembrane Helices Search for a specific query.
Longer loading times are possible. Please use the AA Sequence and Transmembrane Helices Search for a specific query.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
PDB
SCOP
CATH
UNIPROT
ORGANISM
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
48000
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
SUBUNIT
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
decamer
-
the homodecamer has a ring-like structure with five bilobed wings, similar to the structure of the Escherichia coli complex
homodecamer
additional information
additional information
-
formation of the ternary enzyme complex and binding to the ribosome, structure analysis by electron microscopy, 5fold rotational symmetry, overview
additional information
-
two SecS monomers interact intimately and together build up two identical active sites around PLP in a Schiff-base linkage with Lys284. Two SecS dimers further associate to form a homotetramer. The N-terminus, which mediates tetramer formation, and a large insertion that remodels the active site set SecS aside from other members of the family
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
CRYSTALLIZATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
apoenzyme and in complex with Thermoanaerobacter tengcongensis tRNASec, vapor diffusion method
purified recombinant C-terminally His6-tagged SecS, apoenzyme or in complex with phosphate or iodide, sitting-drop vapor diffusion at 20°C, 14 mg/ml protein in 10 mM HEPES-NaOH, pH 7.5, 500 mM NaCl, 2 mM DTT, is mixed with an equal volume of reservoir solution containing 11% v/v ethylene glycol without other buffer components, 2 days, cryoprotection by crystal soaking in 100 mM HEPES-NaOH, pH 7.5, 250 mM NaCl, 1 mM dithiothreitol, and 35% v/v ethylene glycol, X-ray diffraction structure determination and analysis at 1.65-2.38 A resolution
-
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
TEMPERATURE STABILITY
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
45
-
the protein stability is maintained up to 45°C. The enzyme has a thermal transition from 60 to 70°C with a melting temperature of 66°C and an evident plateau above 70°C, in which all secondary structures are lost and the protein is completely denatured
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
ammonium sulfate precipitation, hydroxyapatite column chromatography, Hi Trap Q column chromatography, and Superdex 200 gel filtration
-
centrifugation at 150000 xg, followed by succesive chromatography of the supernatant on DEAE-cellulose, Sephacryl S-300 and Heparin-Toyopearl
-
recombinant C-terminally His6-tagged SecS from Escherichia coli strain Rosetta2(DE3) by nickel affinity chromatography and gel filtration to over 95% purity
-
recombinant SelA from Escherichia coli strain DH5alpha by anion exchange chromatography, ammonium sulfate fractionation, and gel filtration
-
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
expression of SecS as C-terminally His6-tagged enzyme in Escherichia coli strain Rosetta2(DE3)
-
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Forchhammer, K.; Bck, A.
Selenocysteine synthase from Escherichia coli. Analysis of the reaction sequence
J. Biol. Chem.
266
6324-6328
1991
Escherichia coli
brenda
Forchhammer, K.; Leinfelder, W.; Boesmiller, K.; Veprek, B.; Bck, A.
Selenocysteine synthase from Escherichia coli. Nucleotide sequence of the gene (selA) and purification of the protein
J. Biol. Chem.
266
6318-6323
1991
Escherichia coli
brenda
Forchhammer, K.; Boesmiller, K.; Bck, A.
The function of selenocysteine synthase and SELB in the synthesis and incorporation of selenocysteine
Biochimie
73
1481-1486
1991
Escherichia coli
brenda
Engelhardt, H.; Forchhammer, K.; Muller, S.; Goldie, K.N.; Bck, A.
Structure of selenocysteine synthase from Escherichia coli and location of tRNA in the seryl-tRNASec-enzyme complex
Mol. Microbiol.
6
3461-3467
1992
Escherichia coli
brenda
Tanabe, K.; Kanaya, K.; Mizutani, T.
Selenophosphate as a substrate for bovine selenocysteyl-tRNA synthase
Nucleic acids Symp. Ser.
35
287-288
1996
Bos taurus
-
brenda
Tormay, P.; Wilting, R.; Lottspeich, F.; Mehta, P.K.; Christen, P.; Bck, A.
Bacterial selenocysteine synthase
Eur. J. Biochem.
254
655-661
1998
Moorella thermoacetica, Desulfomicrobium baculatum, Escherichia coli, Haemophilus influenzae