EC Number   |
|---|
    2.7.4.9 | - |
| 2.7.4.9 | glutathione beads chromatography |
| 2.7.4.9 | glutathione-Sepharose column chromatography and Mono Q column chromatography |
| 2.7.4.9 | HisTrap column chromatography and Superdex 200 gel filtration |
| 2.7.4.9 | nickel HisTrap column chromatography and Superdex 75 gel filtration |
| 2.7.4.9 | partial |
| 2.7.4.9 | recombinant protein using His-tag |
| 2.7.4.9 | TALON metal affinity resin column chromatography |
| 2.7.4.9 | the stability of the enzyme is maintained during purification by the constant presence of dTMP and 2-mercaptoethanol and by the elimination of substrate-destoying phosphatase activity |
| 2.7.4.9 | truncated recombinant enzyme, full length enzyme could not be crystallized |
