EC Number   |
Reference  |
|---|
   2.7.11.24 | recombinant His-tagged p38 isozyme alpha from Escherichia coli strain BL21(DE3) by nickel affinity and ion exchange chromatography to over 98% purity, removal of the His-tag by thrombin is required for propper enzyme purification and activation |
663620 |
| 2.7.11.24 | recombinant GST-tagged p38 isozyme alpha from Escherichia coli strain BL21(DE3) by two steps of ion exchange chromatography to homogeneity, the recombinant enzyme is detagged |
664894 |
| 2.7.11.24 | purification of synaptosomes |
666131 |
| 2.7.11.24 | recombinant His-tagged p38alpha MAP kinase from Escherichia coli strain BL21(DE3) by nickel chelate affinity chromatohgraphy, dialysis, and ion exchange chromatography to homogeneity, the His-tag is cleaved off by thrombin |
666815 |
| 2.7.11.24 | ERK1 and p38alpha kinase purified by NiNTA column |
677283 |
| 2.7.11.24 | p38alpha active mutants, by gel filtration, on Ni2+-column |
677321 |
| 2.7.11.24 | - |
490760, 491732, 678159, 680249 |
| 2.7.11.24 | gel filtration |
680896 |
| 2.7.11.24 | His-tagged p38 MAP kinase |
690672 |
| 2.7.11.24 | recombinant His-tagged enzyme from Escherichia coli by affinity chromatography |
690853 |
