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Search Purification (Commentary)

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EC Number Purification (Commentary)
Show all pathways known for 2.5.1.32Display the word mapDisplay the reaction diagram Show all sequences 2.5.1.32-
Show all pathways known for 2.5.1.32Display the word mapDisplay the reaction diagram Show all sequences 2.5.1.32partial
Show all pathways known for 2.5.1.32Display the word mapDisplay the reaction diagram Show all sequences 2.5.1.32Sephadex G-25 gel filtration and ammonium sulfate fractionation
Show all pathways known for 2.5.1.32Display the word mapDisplay the reaction diagram Show all sequences 2.5.1.32the purification protocol presents detailed instructions how to purify several milligrams of active enzyme from bacterial lysates, which includes initial recombinant enzyme enrichment through inclusion body purification, chaotropic unfolding, refolding in presence of detergents and purification through immobilized metal affinity chromatography
Show all pathways known for 2.5.1.32Display the word mapDisplay the reaction diagram Show all sequences 2.5.1.32to facilitate purification of the recombinant enzyme, the structural gene for Psase is modified by site-directed mutagenesis to incorporate a C-terminal Glu-Glu-Phe tripeptide to allow purification by immunoaffinity chromatography on an immobilized monoclonal anti-alpha-tubulin antibody YL1/2 column
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