EC Number   |
|---|
   2.4.2.6 | - |
| 2.4.2.6 | ammonium sulfate precipitation and Sephacryl S-200 gel filtration |
| 2.4.2.6 | Bio-Scale Mini UNOsphere Q column chromatography, and Superose 12 gel filtration |
| 2.4.2.6 | DRTase I and DRTAse II |
| 2.4.2.6 | preclearing of bacterial lysate with 40% ammonium sulfate and subsequent precipitation of proteins with 90% ammonium sulfate, gel filtration of dissolved protein on Sephacryl S-200 column, combination of fractions with N-deoxyribosyltransferase activity, precipitation of proteins with 90% ammonium sulfate, dialysis of dissolved proteins and concentration by ultrafiltration, second gel filtration on Sephacryl S-200 column, combination of active fractions and concentration by ultrafiltration, recovery of 70.4% of activity, storage at -20°C in 50% glycerol |
| 2.4.2.6 | recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography |
| 2.4.2.6 | recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography and gel filtration |
| 2.4.2.6 | recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography and preparative gel fitration |
| 2.4.2.6 | recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography and tag cleavage through thrombin |
| 2.4.2.6 | recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography, method optimization for LaNDT |
