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Information on EC 2.4.2.6 - nucleoside deoxyribosyltransferase
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2.4.2.6 nucleoside deoxyribosyltransferaseIUBMB Comments
Base1 and base2 represent various purines and pyrimidines.
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Word Map
The enzyme appears in viruses and cellular organisms
Synonyms
2'-deoxyribosyltransferase, 2'-N-deoxyribosyltransferase, Chro_1188, CtNDT, deoxynucleoside 5-monophosphate N-glycosidase, deoxyribose transferase, deoxyribosyltransferase, nucleoside, DRTase I, DRTase II, GQR93_14380, 
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SYNONYM 
ORGANISM
UNIPROT
COMMENTARY 
LITERATURE
2'-deoxyribosyltransferase
2'-N-deoxyribosyltransferase
Chro_1188
CtNDT
deoxyribose transferase
-
-
-
-
deoxyribosyltransferase, nucleoside
-
-
-
-
DRTase II
-
catalyzes the transfer of the deoxyribosyl moiety between purines or pyrimidines as well as from a purine to a pyrimidine
GQR93_14380
LaNDT
LhNDT
LmPDT
LrNDT
N-deoxyribosyltransferase II
NdRT-II
-
-
-
-
NDT
NTD
nucleoside 2'-deoxyribosyltransferase
nucleoside 2'-deoxyribosyltransferase type II
nucleoside 2-deoxyribosyltransferase
nucleoside deoxyribosyltransferase I
-
purine nucleoside:purine deoxyribosyltransferase:strictly specific for transfer between purine bases
nucleoside deoxyribosyltransferase II
nucleoside N-deoxyribosyltransferase
nucleoside trans-N-deoxyribosylase
-
-
-
-
PDT
purine nucleoside 2'-deoxyribosyltransferase
purine(pyrimidine) nucleoside:purine(pyrimidine) deoxyribosyl transferase
-
-
-
-
Tb927.5.1360
TbPDT
trans-deoxyribosylase
-
-
-
-
trans-N-deoxyribosylase
trans-N-glycosidase
-
-
-
-
transdeoxyribosylase
-
-
-
-
type I NDT
nucleoside deoxyribosyltransferase II
-
purine(pyrimidine) nucleoside:purine(pyrimidine) deoxyribosyltransferase:catalyzes the transfer of the deoxyribosyl moiety between purines or pyrimidines as well as from a purine to a pyrimidine
trans-N-deoxyribosylase
-
-
-
-
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REACTION
REACTION DIAGRAM
COMMENTARY
ORGANISM
UNIPROT
LITERATURE
2-deoxy-D-ribosyl-base1 + base2 = 2-deoxy-D-ribosyl-base2 + base1
ping-pong bi bi mechanism
-
2-deoxy-D-ribosyl-base1 + base2 = 2-deoxy-D-ribosyl-base2 + base1
Glu105, Asp-99 and Asn-172 are catalytic active site residues
-
2-deoxy-D-ribosyl-base1 + base2 = 2-deoxy-D-ribosyl-base2 + base1
Glu90 is a catalytic active site residue, ligand binding and active site structures, overview
-
2-deoxy-D-ribosyl-base1 + base2 = 2-deoxy-D-ribosyl-base2 + base1
reaction mechanism with oxocarbenium ion as the reaction intermediate, overview
2-deoxy-D-ribosyl-base1 + base2 = 2-deoxy-D-ribosyl-base2 + base1
-
-
-
-
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REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
pentosyl group transfer
transglycosylation
pentosyl group transfer
-
-
-
-
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PATHWAY SOURCE
PATHWAYS
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SYSTEMATIC NAME
IUBMB Comments
nucleoside:purine(pyrimidine) deoxy-D-ribosyltransferase
Base1 and base2 represent various purines and pyrimidines.
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CAS REGISTRY NUMBER
COMMENTARY
9026-86-2
-
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SUBSTRATE
PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate)
(Substrate)
LITERATURE
(Substrate)
(Substrate)
COMMENTARY
(Product)
(Product)
LITERATURE
(Product)
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
r=reversible
ir=irreversible
?=not specified
2',3'-dideoxyadenosine + 2,6-diaminopurine
2,6-diaminopurine-2',3'-dideoxyriboside + adenine
2',3'-dideoxyadenosine + 6-mercaptopurine
6-mercaptopurine-2',3'-dideoxyriboside + adenine
low activity
-
-
?
2',3'-dideoxyadenosine + 6-methoxyguanine
6-methoxy-2',3'-dideoxyguanosine + adenine
-
-
-
?
2',3'-dideoxyadenosine + 6-methylpurine
6-methylpurine-2',3'-dideoxyriboside + adenine
low activity
-
-
?
2',3'-dideoxyadenosine + thymine
2',3'-dideoxythymidine + adenine
-
-
-
?
2',3'-dideoxycytidine + thymine
2',3'-dideoxythymidine + cytosine
-
-
-
?
2',3'-dideoxyinosine + 2,6-diaminopurine
2,6-diaminopurine-2',3'dideoxyriboside + inosine
-
-
-
?
2',3'-dideoxyinosine + 6-chloropurine
6-chloropurine-2',3'-dideoxyriboside + inosine
low activity
-
-
?
2',3'-dideoxyinosine + 6-mercaptopurine
6-mercaptopurine-2',3'-dideoxyriboside + inosine
low activity
-
-
?
2',3'-dideoxyinosine + 6-methoxyguanine
6-methoxy-2',3'-dideoxyguanine + inosine
low activity
-
-
?
2',3'-dideoxyinosine + 6-methylpurine
6-methylpurine-2',3'-dideoxyriboside + inosine
low activity
-
-
?
2',3'didehydro-2',3'-dideoxythymidine + adenine
2',3'didehydro-2',3'-dideoxyadenosine + thymine
2'-deoxyadenosine + 5-fluorouracil
2'-deoxy-D-ribosyl-5-fluorouracil + adenine
reaction is catalyzed by mutants F13R/D92S/N123S and F13Q/D92S/N123S
-
-
?
2'-deoxyadenosine + hypoxanthine
2'-deoxyinosine + adenine
-
-
-
?
2'-deoxyadenosine + thymine
2'-deoxythymidine + adenine
-
-
-
?
2'-deoxycytidine + 2,6-diaminopurine
2,6-diaminopurine 2-deoxy-D-ribose 5-phosphate + cytosine
reaction is catalyzed by mutants F13R/D92S/N123S and F13Q/D92S/N123S
-
-
?
2'-deoxycytidine + 5-bromouracil
2'-deoxy-5-bromouridine + cytosine
reaction is catalyzed by mutants F13R/D92S/N123S and F13Q/D92S/N123S
-
-
?
2'-deoxycytidine + 6-chloroguanine
cytosine + 2'-deoxy-6-chloroguanosine
-
DRTase II
-
-
?
2'-deoxycytidine + 6-chloropurine
6-chloropurine 2-deoxy-D-ribose 5-phosphate + cytosine
reaction is catalyzed by mutants F13R/D92S/N123S and F13Q/D92S/N123S
-
-
?
2'-deoxycytidine + adenosine
2'-deoxyadenosine + cytosine
-
-
-
-
?
2'-deoxycytidine + thymine
2'-deoxythymidine + cytosine
-
-
-
?
2'-deoxyguanosine + 2-fluoroadenine
2-fluoro-2'-deoxyadenosine + guanine
-
-
-
?
2'-deoxyguanosine + 6-chloropurine
6-chloropurine-2'-deoxyriboside + guanine
-
-
-
?
2'-deoxyguanosine + 6-mercaptopurine
6-mercaptopurine-2'-deoxyriboside + guanine
-
-
-
?
2'-deoxyguanosine + 6-methoxyguanine
6-methoxy-2'-deoxyguanosine + guanine
-
-
-
?
2'-deoxyguanosine + 6-methylpurine
6-methylpurine-2'-deoxyriboside + guanine
-
-
-
?
2'-deoxyguanosine + benzimidazole
benzimidazole 2'-deoxyriboside + guanine
-
-
-
?
2'-deoxyguanosine + hypoxanthine
2'-deoxyinosine + guanine
-
-
-
?
2'-deoxyinosine + cytosine
2'-deoxycytidine + hypoxanthine
-
-
-
?
2'-deoxyinosine + uracil
2'-deoxyuridine + hypoxanthine
-
-
-
?
2'-deoxythymidine + 5-azacytosine
5-aza-2'-deoxycytosine + thymine
-
-
-
?
2'-deoxyuridine + 2,6-diaminopurine
2,6-diaminopurine 2-deoxy-D-ribose 5-phosphate + uracil
2'-deoxyuridine + 5-ethyluracil
5-ethyl-2'-deoxyuridine + uracil
-
-
-
?
2'-deoxyuridine + 5-methylcytosine
5-methyl-2'-deoxycytidine + uracil
-
-
-
?
2'-deoxyuridine + hypoxanthine
2'-deoxyinosine + uracil
-
-
-
?
2'-fluoro-2'-deoxyadenosine + guanine
2'-fluoro-2'-deoxyguanosine + adenine
-
-
-
?
2-fluoro-2'-deoxyinosine + 2,6-diaminopurine
2,6-diaminopurine 2'-deoxyriboside + 2-fluoroinosine
-
-
-
?
2-fluoro-2'-deoxyinosine + 2-chloroadenine
2-chloro-2'-deoxyadenosine + 2-fluoroinosine
-
-
-
?
2-fluoro-2'-deoxyinosine + 2-fluoroadenine
2-fluoro-2'-deoxyadenosine + 2-fluoroinosine
-
-
-
?
2-fluoro-2'-deoxyinosine + 6-chloropurine
6-chloropurine 2'-deoxyriboside + 2-fluoroinosine
low activity
-
-
?
2-fluoro-2'-deoxyinosine + 6-mercaptopurine
6-mercaptopurine 2'-deoxyriboside + 2-fluoroinosine
-
-
-
?
2-fluoro-2'-deoxyinosine + 6-methoxyguanine
6-methoxyguanine 2'-deoxyriboside + 2-fluoroinosine
-
-
-
?
2-fluoro-2'-deoxyinosine + 6-methylpurine
6-methylpurine 2'-deoxyriboside + 2-fluoroinosine
low activity
-
-
?
2-fluoro-2'-deoxyinosine + adenine
2'-deoxyadenosine + 2-fluoroinosine
-
-
-
?
2-fluoro-2'-deoxyinosine + guanine
2'-deoxyguanosine + 2-fluoroinosine
-
-
-
?
5'-fluoro-5'-thymidine + adenine
5'-fluoro-5'-deoxythymine + adenosine
-
deoxyribosyltransferase II
-
-
?
5-iodouracil + 2'-deoxyadenosine
5-iodo-2'-deoxyuridine + adenine
immobilized recombinant enzyme
-
-
?
5-iodouracil + 2'-deoxyuridine
5-iodo-2'-deoxyuridine + uracil
immobilized recombinant enzyme
-
-
?
9-beta-D-arabinofuranosyl adenine + 2,6-diaminopurine
9-beta-D-arabinofuranosyl 2,6-diaminopurine + adenine
-
-
-
?
9-beta-D-arabinofuranosyl adenine + 2-chloroadenine
9-beta-D-arabinofuranosyl 2-chloroadenine + adenine
-
-
-
?
9-beta-D-arabinofuranosyl adenine + 2-fluoroadenine
9-beta-D-arabinofuranosyl 2-fluoroadenine + adenine
-
-
-
?
9-beta-D-arabinofuranosyl adenine + 6-chloropurine
9-beta-D-arabinofuranosyl 6-chloropurine + adenine
-
-
-
?
9-beta-D-arabinofuranosyl adenine + 6-methoxyguanine
9-beta-D-arabinofuranosyl 6-methoxyguanine + adenine
-
-
-
?
9-beta-D-arabinofuranosyl adenine + 6-methylpurine
9-beta-D-arabinofuranosyl 6-methylpurine + adenine
-
-
-
?
9-beta-D-arabinofuranosyl adenine + guanine
9-beta-D-arabinofuranosyl guanine + adenine
-
-
-
?
9-beta-D-arabinofuranosyl adenine + hypoxanthine
9-beta-D-arabinofuranosyl hypoxanthine + adenine
-
-
-
?
9-beta-D-arabinofuranosyl guanine + 2,6-diaminopurine
9-beta-D-arabinofuranosyl 2,6-diaminopurine + guanine
-
-
-
?
9-beta-D-arabinofuranosyl guanine + 2-chloroadenine
9-beta-D-arabinofuranosyl 2-chloroadenine + guanine
-
-
-
?
9-beta-D-arabinofuranosyl guanine + 2-fluoroadenine
9-beta-D-arabinofuranosyl 2-fluoroadenine + guanine
-
-
-
?
9-beta-D-arabinofuranosyl guanine + 6-chloropurine
9-beta-D-arabinofuranosyl 6-chloropurine + guanine
low activity
-
-
?
9-beta-D-arabinofuranosyl guanine + 6-mercaptopurine
9-beta-D-arabinofuranosyl 6-mercaptopurine + guanine
-
-
-
?
9-beta-D-arabinofuranosyl guanine + 6-methoxyguanine
9-beta-D-arabinofuranosyl 6-methoxyguanine + guanine
-
-
-
?
9-beta-D-arabinofuranosyl guanine + 6-methylpurine
9-beta-D-arabinofuranosyl 6-methylpurine + guanine
-
-
-
?
9-beta-D-arabinofuranosyl guanine + adenine
9-beta-D-arabinofuranosyl adenine + guanine
-
-
-
?
9-beta-D-arabinofuranosyl guanine + hypoxanthine
9-beta-D-arabinofuranosyl hypoxanthine + guanine
-
-
-
?
9-beta-D-arabinofuranosyl guanine + hypoxanthine
?
low activity
-
-
?
adenine + deoxycytidine
deoxyadenosine + cytosine
-
64.0% conversion after 4 h at standard reaction conditions: 2 mM each substrate, 0.013 U NTD, pH 7.5, 25°C, 40 mM (NH4)2SO4, 0.5 mg/ml bovine serum albumin
-
-
r
adenine + deoxyguanosine
deoxyadenosine + guanine
-
46.6% conversion after 4 h at standard reaction conditions: 2 mM each substrate, 0.013 U NTD, pH 7.5, 25°C, 40 mM (NH4)2SO4, 0.5 mg/ml bovine serum albumin
-
-
r
adenine + deoxyinosine
deoxyadenosine + hypoxanthine
-
57.3% conversion after 4 h at standard reaction conditions: 2 mM each substrate, 0.013 U NTD, pH 7.5, 25°C, 40 mM (NH4)2SO4, 0.5 mg/ml bovine serum albumin
-
-
r
adenine + deoxyuridine
deoxyadenosine + uracil
-
75.4% conversion after 4 h at standard reaction conditions: 2 mM each substrate, 0.013 U NTD, pH 7.5, 25°C, 40 mM (NH4)2SO4, 0.5 mg/ml bovine serum albumin
-
-
r
adenine + thymidine
deoxyadenosine + thymine
-
74.6% conversion after 4 h at standard reaction conditions: 2 mM each substrate, 0.013 U NTD, pH 7.5, 25°C, 40 mM (NH4)2SO4, 0.5 mg/ml bovine serum albumin
-
-
r
cytosine + deoxyadenosine
deoxycytidine + adenine
-
27.7% conversion after 4 h at standard reaction conditions: 2 mM each substrate, 0.013 U NTD, pH 7.5, 25°C, 40 mM (NH4)2SO4, 0.5 mg/ml bovine serum albumin
-
-
r
cytosine + deoxyguanosine
deoxycytidine + guanine
-
25.7% conversion after 4 h at standard reaction conditions: 2 mM each substrate, 0.013 U NTD, pH 7.5, 25°C, 40 mM (NH4)2SO4, 0.5 mg/ml bovine serum albumin
-
-
r
cytosine + deoxyinosine
deoxycytidine + hypoxanthine
-
32.0% conversion after 4 h at standard reaction conditions: 2 mM each substrate, 0.013 U NTD, pH 7.5, 25°C, 40 mM (NH4)2SO4, 0.5 mg/ml bovine serum albumin
-
-
r
cytosine + deoxyuridine
deoxycytidine + uracil
-
62.9% conversion after 4 h at standard reaction conditions: 2 mM each substrate, 0.013 U NTD, pH 7.5, 25°C, 40 mM (NH4)2SO4, 0.5 mg/ml bovine serum albumin
-
-
r
dAMP + 2,6-dichloropurine
adenine + 2,6-dichloropurin-9-yl-2'-deoxy-beta-D-ribose 5'-phosphate
-
-
-
-
?
dCMP + 2,6-dichloropurine
2,6-dichloropurin-9-yl-2'-deoxy-beta-D-ribose 5'-phosphate + cytosine
reaction is catalyzed by mutants F13R/D92S/N123S and F13Q/D92S/N123S
-
-
?
deoxyadenosine + 2-amino 6-methylmercaptopurine
adenine + 9-(2-deoxy-beta-D-ribofuranosyl)-2-amino-6-methylmercaptopurine
-
-
-
-
?
deoxyadenosine + 6-chloroguanine
adenine + 2'-deoxy-6-chloroguanosine
-
-
-
-
?
deoxyadenosine + 6-chloropurine
adenine + 9-(2-deoxy-beta-D-ribofuranosyl)-6-chloropurine
-
-
-
-
?
deoxyadenosine + 6-dimethylaminopurine
adenine + 9-(2-deoxy-beta-D-ribofuranosyl)-6-dimethylaminopurine
-
-
-
-
?
deoxyadenosine + 6-methylpurine
adenine + 9-(2-deoxy-beta-D-ribofuranosyl)-6-methylpurine
-
-
-
-
?
deoxyadenosine + ethyl 4-amino-1H-imidazole-5-carboxylate
adenine + ethyl 4-amino-1-(2-deoxy-b-D-erythro-pentofuranosyl)-1H-imidazole-5-carboxylate
-
-
-
-
?
deoxyadenosine + hypoxanthine
adenine + deoxyinosine + 7-(beta-D-2'-deoxyribufuranosyl)hypoxanthine
deoxycytidine + 1,7-dimethylguanine
cytosine + deoxy-1,7-dimethylguanosine
-
DRTase II
-
-
?
deoxycytidine + 1,N2-epsilon-guanine
cytosine + ?
-
deoxyribosylation at the N9 atom
-
-
?
deoxycytidine + 1,N6-epsilon-adenine
cytosine + ?
-
deoxyribosylation at the N9 atom
-
-
?
deoxycytidine + 3,N4-epsilon-cytosine
cytosine + ?
-
deoxyribosylation at the N9 atom
-
-
?
deoxycytidine + 5,6,7,9-tetrahydro-7-acetoxy-9-oxoimidazo[1,2-a]purine
cytosine + ?
-
deoxyribosylation at the N9 atom
-
-
?
deoxycytidine + C8-aminoguanine
cytosine + 2'-deoxy-8-aminoguanosine
-
deoxyribosylation at the N9 atom
-
-
?
deoxycytidine + C8-methylguanine
cytosine + 2'-deoxy-8-methylguanosine
-
deoxyribosylation at the N9 atom
-
-
?
deoxycytidine + hypoxanthine
cytosine + deoxyinosine
-
-
-
-
r
deoxycytidine + N2,3-epsilon-guanine
cytosine + ?
-
deoxyribosylation at the N9 atom
-
-
?
deoxycytidine + N2-(2-oxoethyl)guanine
cytosine + 2'-deoxy-N2-(2-oxoethyl)guanosine
-
deoxyribosylation at the N9 atom
-
-
?
deoxycytidine + pyrimido[1,2-a]purin-10(3H)-one
cytosine + ?
-
deoxyribosylation at the N9 atom
-
-
?
deoxyinosine + 1,7-dimethylguanine
hypoxanthine + deoxy-1,7-dimethylguanosine
-
DRTase I
-
-
?
deoxyinosine + 6-chloroguanine
hypoxanthine + deoxy-6-chloroguanosine
-
DRTase I
-
-
?
deoxyinosine + 8-azaguanine
hypoxanthine + deoxy-8-azaguanosine
-
-
-
-
r
deoxyinosine + 8-azaxanthine
hypoxanthine + deoxy-8-azaxanthosine
-
-
-
-
r
deoxyinosine + uric acid
hypoxanthine + 9-(2-deoxy-beta-D-ribofuranosyl)-7,9-dihydro-3H-purine-2,6,8-trione
-
-
-
-
r
hypoxanthine + 2',3'-dideoxyuridine
2',3'-dideoxyinosine + uracil
-
-
-
?
hypoxanthine + deoxyadenosine
deoxyinosine + adenine
-
38.0% conversion after 4 h at standard reaction conditions: 2 mM substrate, 0.013 U NTD, pH 7.5, 25°C, 40 mM (NH4)2SO4, 0.5 mg/ml bovine serum albumin
-
-
r
hypoxanthine + deoxycytidine
deoxyinosine + cytosine
-
51.9% conversion after 4 h at standard reaction conditions: 2 mM each substrate, 0.013 U NTD, pH 7.5, 25°C, 40 mM (NH4)2SO4, 0.5 mg/ml bovine serum albumin
-
-
r
hypoxanthine + deoxyguanosine
deoxyinosine + guanine
-
25.2% conversion after 4 h at standard reaction conditions: 2 mM substrate, 0.013 U NTD, pH 7.5, 25°C, 40 mM (NH4)2SO4, 0.5 mg/ml bovine serum albumin
-
-
r
hypoxanthine + deoxyuridine
deoxyinosine + uracil
-
65.6% conversion after 4 h at standard reaction conditions: 2 mM substrate, 0.013 U NTD, pH 7.5, 25°C, 40 mM (NH4)2SO4, 0.5 mg/ml bovine serum albumin
-
-
r
hypoxanthine + thymidine
deoxyinosine + thymine
-
76.8% conversion after 4 h at standard reaction conditions: 2 mM substrate, 0.013 U NTD, pH 7.5, 25°C, 40 mM (NH4)2SO4, 0.5 mg/ml bovine serum albumin
-
-
r
inosine + adenine
adenosine + hypoxanthine
cf. EC 2.4.2.5
-
-
?
purine base + 2-deoxy-D-ribosyl-base
purine deoxyribonucleoside + base
-
-
preferred over pyrimidine deoxyribonucleoside synthesis
-
r
pyrimidine base + 2-deoxy-D-ribosyl-base
pyrimidine deoxyribonucleoside + base
-
-
-
-
r
thymidine + 1-deaza-8-aza-adenine
thymine + 2'-deoxy-1-deaza-8-aza-adenosine
-
21% of substrate converted into deoxynucleoside after 2 h
-
-
?
thymidine + 1-deaza-adenine
thymine + 2'-deoxy-1-deaza-adenosine
-
6.2% of substrate converted into deoxynucleoside after 2 h
-
-
?
thymidine + 2,4-di-tert-butoxy-5-(1H-imidazol-4-yl)pyrimidine
2,4-di-tert-butoxy-5-(1-beta-D-ribofuranosyl-1H-imidazol-4-yl)pyrimidine + thymine
-
-
-
-
?
thymidine + 2,6-diaminopurine
thymine + 9-(2-deoxy-beta-D-ribofuranosyl)-2,6-diaminopurine
-
53.4% of substrate converted into deoxynucleoside after 2 h
-
-
?
thymidine + 2-(1H-imidazol-4-yl)benzaldehyde
2-[1-(2-deoxy-beta-D-ribofuranosyl)-1H-imidazol-4-yl]-benzaldehyde + thymine
-
-
-
-
?
thymidine + 2-amino-6-methyl-mercaptopurine
thymine + 9-(2-deoxy-beta-D-ribofuranosyl)-2-amino-6-methylmercaptopurine
-
-
-
-
r
thymidine + 2-aminopurine
thymine + 9-(2-deoxy-beta-D-ribofuranosyl)-2-aminopurine
-
-
-
-
r
thymidine + 2-fluoro-3-(1H-imidazol-4-yl)pyridine
2-fluoro-3-[1-(2-deoxy-beta-D-ribofuranosyl)-1H-imidazol-4-yl]-pyridine + thymine
-
-
-
-
?
thymidine + 3-(1H-imidazol-4-yl)pyridine
3-[1-(2-deoxy-beta-D-ribofuranosyl)-1H-imidazol-4-yl]-pyridine + thymine
-
-
-
-
?
thymidine + 3-bromo-5-(1H-imidazol-4-yl)pyridine
5-bromo-3-[1-(2-deoxy-beta -D-ribofuranosyl)-1H-imidazol-4-yl]-pyridin + thymine
-
-
-
-
?
thymidine + 3-deaza-8-aza-2-aminopurine
thymine + 9-(2-deoxy-beta-D-ribofuranosyl)-3-deaza-8-aza-aminopurine
-
39.7% of substrate converted into deoxynucleoside after 2 h
-
-
?
thymidine + 3-deaza-8-aza-adenine
thymine + 2'-deoxy-3-deaza-8-aza-adenosine
-
3.5% of substrate converted into deoxynucleoside after 2 h
-
-
?
thymidine + 4-(1-benzofuran-2-yl)-1H-imidazole
1-(2-deoxy-beta-D-ribofuranosyl)-4-(benzofuran-2-yl)-1H-imidazole + thymine
-
-
-
-
?
thymidine + 4-(1H-imidazol-4-yl)benzonitrile
4-[1-(2-deoxy-beta-D-ribofuranosyl)-1H-imidazol-4-yl]-benzonitrile + thymine
-
-
-
-
?
thymidine + 4-(2,4-difluorophenyl)-1H-imidazole
1-(2-deoxy-beta-D-ribofuranosyl)-4-(2,4-difluorophenyl)-1H-imidazole + thymine
-
-
-
-
?
thymidine + 4-(4-ethoxyphenyl)-1H-imidazole
1-(2-deoxy-beta-D-ribofuranosyl)-4-(4-ethoxyphenyl)-1H-imidazole + thymine
-
-
-
-
?
thymidine + 4-(4-methoxyphenyl)-1H-imidazole
1-(2-deoxy-beta-D-ribofuranosyl)-4-(4-methoxyphenyl)-1H-imidazole + thymine
-
-
-
-
?
thymidine + 4-(6-methoxynaphthalen-2-yl)-1H-imidazole
1-(2-deoxy-beta-D-ribofuranosyl)-4-(6-methoxynaphthalen-2-yl)-1H-imidazole + thymine
-
-
-
-
?
thymidine + 4-(dibenzo[b,d]furan-4-yl)-1H-imidazole
1-(2-deoxy-beta-D-ribofuranosyl)-4-(dibenzo[b,d]furan-4-yl)-1H-imidazole + thymine
-
-
-
-
?
thymidine + 4-(naphthalen-2-yl)-1H-imidazole
1-(2-deoxy-beta-D-ribofuranosyl)-4-(naphthalen-2-yl)-1H-imidazole + thymine
-
-
-
-
?
thymidine + 4-([1,1'-biphenyl]-3-yl)-1H-imidazole
1-(2-deoxy-beta-D-ribofuranosyl)-4-(biphenyl-3-yl)-1H-imidazole + thymine
-
-
-
-
?
thymidine + 4-([1,1'-biphenyl]-4-yl)-1H-imidazole
1-(2-deoxy-beta-D-ribofuranosyl)-4-(biphenyl-4-yl)-1H-imidazole + thymine
-
-
-
-
?
thymidine + 4-([11,21:23,31-terphenyl]-25-yl)-1H-imidazole
1-(2-deoxy-beta-D-ribofuranosyl)-4-[(3,5-diphenyl)phenyl]-1H-imidazole + thymine
-
-
-
-
?
thymidine + 4-amino-5-carboxamide-imidazole
thymine + ?
-
12.3% of substrate converted into deoxynucleoside after 2 h
-
-
?
thymidine + 4-amino-pyrazolopyrimidine
thymine + ?
-
0.37% of substrate converted into deoxynucleoside after 2 h
-
-
?
thymidine + 4-phenyl-1H-imidazole
1-(2-deoxy-beta-D-ribofuranosyl)-4-phenyl-1H-imidazole + thymine
-
-
-
-
?
thymidine + 5,6-dimethyl-benzimidazole
thymine + ?
-
37.7% of substrate converted into deoxynucleoside after 2 h
-
-
?
thymidine + 5-(1H-imidazol-4-yl)-1H-indazole
1-(2-deoxy-beta-D-ribofuranosyl)-4-(1H-indazol-5-yl)-1H-imidazole + thymine
-
-
-
-
?
thymidine + 5-(1H-imidazol-4-yl)-1H-indole
1-(2-deoxy-beta-D-ribofuranosyl)-4-(1H-indol-5-yl)-1H-imidazole + thymine
-
-
-
-
?
thymidine + 5-(1H-imidazol-4-yl)-2,4-dimethoxypyrimidine
2,4-dimethoxy-5-[1-(2-deoxy-beta-D-ribofuranosyl)-1H-imidazol-4-yl]-pyrimidine + thymine
-
-
-
-
?
thymidine + 5-(1H-imidazol-4-yl)pyrimidin-2-amine
2-amino-5-[1-(2-deoxy-beta-D-ribofuranosyl)-1H-imidazol-4-yl]-pyrimidine + thymine
-
-
-
-
?
thymidine + 5-(1H-imidazol-4-yl)pyrimidine
5-[1-(2-deoxy-beta-D-ribofuranosyl)-1H-imidazol-4-yl]-pyrimidine + thymine
-
-
-
-
?
thymidine + 5-bromouracil
thymine + 2'-deoxy-5-bromouridine
-
-
-
-
?
thymidine + 5-fluorouracil
thymine + 2'-deoxy-5-fluorouridine
-
-
-
-
?
thymidine + 5-hydroxyuracil
thymine + 2'-deoxy-5-hydroxyuridine
-
-
-
-
?
thymidine + 5-methylcytosine
thymine + 2'-deoxy-5-methylcytosine
-
-
-
-
?
thymidine + 5-nitrouracil
thymine + 2'-deoxy-5-nitrouridine
-
-
-
-
?
thymidine + 6-benzylaminopurine
thymine + 9-(2-deoxy-beta-D-ribofuranosyl)-6-benzylaminopurine
-
-
-
-
r
thymidine + 6-chloropurine
thymine + 9-(2-deoxy-beta-D-ribofuranosyl)-6-chloropurine
-
-
-
-
r
thymidine + 6-dimethylaminopurine
thymine + 9-(2-deoxy-beta-D-ribofuranosyl)-6-dimethylaminopurine
-
-
-
-
r
thymidine + 6-iodopurine
thymine + 9-(2-deoxy-beta-D-ribofuranosyl)-6-iodopurine
-
65.6% of substrate converted into deoxynucleoside after 2 h
-
-
?
thymidine + 6-mercaptoguanine
thymine + 6-mercaptoguanosine
-
52.1% of substrate converted into deoxynucleoside after 2 h
-
-
?
thymidine + 6-mercatopurine
thymine + 9-(2-deoxy-beta-D-ribofuranosyl)-6-mercaptopurine
-
56.5% of substrate converted into deoxynucleoside after 2 h
-
-
?
thymidine + 6-methoxypurine
thymine + 9-(2-deoxy-beta-D-ribofuranosyl)-6-methoxypurine
-
-
-
-
r
thymidine + 6-methylaminopurine
thymine + 9-(2-deoxy-beta-D-ribofuranosyl)-6-methylaminopurine
-
-
-
-
r
thymidine + 6-methylpurine
thymine + 9-(2-deoxy-beta-D-ribofuranosyl)-6-methylpurine
-
-
-
-
r
thymidine + 6-methylthio-8-trifluoromethylpurine
thymine + 9-(2-deoxy-beta-D-ribofuranosyl)-6-methylthio-8-trifluoromethylpurine
-
-
-
-
?
thymidine + 6-n-hexylaminopurine
thymine + 9-(2-deoxy-beta-D-ribofuranosyl)-6-n-hexylaminopurine
-
-
-
-
r
thymidine + 6-n-hexylmercaptopurine
thymine + 9-(2-deoxy-beta-D-ribofuranosyl)-6-n-hexylmercaptopurine
-
-
-
-
r
thymidine + 6-phenylaminopurine
thymine + 9-(2-deoxy-beta-D-ribofuranosyl)-6-phenylaminopurine
-
-
-
-
r
thymidine + 7-hydroxy-1,2,5-selenadiazolopyridine
thymine + ?
-
29.2% of substrate converted into deoxynucleoside after 2 h
-
-
?
thymidine + 8-aza-2,6-diaminopurine
thymine + 9-(2-deoxy-beta-D-ribofuranosyl)-8-aza-2,6-diaminopurine
-
59.6% of substrate converted into deoxynucleoside after 2 h
-
-
?
thymidine + 8-azaguanine
thymine + 2'-deoxy-8-azaguanosine
-
16.0% of substrate converted into deoxynucleoside after 2 h
-
-
?
thymidine + 8-azaxanthine
thymine + 2'-deoxy-8-azaxanthosine
-
-
-
-
?
thymidine + 8-bromoadenine
thymine + 2'-deoxy-8-bromoadenosine
-
-
-
?
thymidine + 8-chloroadenine
thymine + 2'-deoxy-8-chloroadenosine
-
-
3-deoxyribonucleoside + 9-deoxyribonucleoside
?
thymidine + 8-chlorotheophylline
thymine + 9-(2-deoxy-beta-D-ribofuranosyl)-8-chlorotheophylline
-
-
-
-
?
thymidine + 8-methyladenine
thymine + 2'-deoxy-8-methyladenosine
-
-
-
-
?
thymidine + 8-trifluoromethyladenine
thymine + 2'-deoxy-8-trifluoromethyladenosine
-
-
-
-
?
thymidine + benzimidazole
thymine + ?
-
33.1% of substrate converted into deoxynucleoside after 2 h
-
-
?
thymidine + methyl 3-(1H-imidazol-4-yl)benzoate
methyl 3-[1-(2-deoxy-beta-D-ribofuranosyl)-1H-imidazol-4-yl]-benzoate + thymine
-
-
-
-
?
thymidine + purine
thymine + 9-(2-deoxy-beta-D-ribofuranosyl)-purine
-
-
-
-
r
thymidine + theophylline
thymine + 2'-deoxytheophylline riboside
-
-
-
-
?
thymidine + xanthine
9-deoxyribosylxanthine + 7-deoxyribosylxanthine
-
-
20% 9-deoxyribosylxanthine + 80% 7-deoxyribosylxanthine
?
thymidine + [4-(1H-imidazol-4-yl)phenyl]methanol
1-(2-deoxy-beta-D-ribofuranosyl)-4-(4-hydroxymethylphenyl)-1H-imidazole + thymine
-
-
-
-
?
thymine + deoxyadenosine
thymidine + adenine
-
22.7% conversion after 4 h at standard reaction conditions: 2 mM each substrate, 0.013 U NTD, pH 7.5, 25°C, 40 mM (NH4)2SO4, 0.5 mg/ml bovine serum albumin
-
-
r
thymine + deoxycytidine
thymidine + cytosine
-
38.3% conversion after 4 h at standard reaction conditions: 2 mM each substrate, 0.013 U NTD, pH 7.5, 25°C, 40 mM (NH4)2SO4, 0.5 mg/ml bovine serum albumin
-
-
r
thymine + deoxyguanosine
thymidine + guanine
-
17.7% conversion after 4 h at standard reaction conditions: 2 mM each substrate, 0.013 U NTD, pH 7.5, 25°C, 40 mM (NH4)2SO4, 0.5 mg/ml bovine serum albumin
-
-
r
thymine + deoxyinosine
thymidine + hypoxanthine
-
19.5% conversion after 4 h at standard reaction conditions: 2 mM each substrate, 0.013 U NTD, pH 7.5, 25°C, 40 mM (NH4)2SO4, 0.5 mg/ml bovine serum albumin, equilibrium reached after 2 h
-
-
r
thymine + deoxyuridine
thymidine + uracil
-
53.7% conversion after 4 h at standard reaction conditions: 2 mM each substrate, 0.013 U NTD, pH 7.5, 25°C, 40 mM (NH4)2SO4, 0.5 mg/ml bovine serum albumin
-
-
r
uracil + deoxyadenosine
deoxyuridine + adenine
-
17.1% conversion after 4 h at standard reaction conditions: 2 mM each substrate, 0.013 U NTD, pH 7.5, 25°C, 40 mM (NH4)2SO4, 0.5 mg/ml bovine serum albumin
-
-
r
uracil + deoxycytidine
deoxyuridine + cytosine
-
37.7% conversion after 4 h at standard reaction conditions: 2 mM each substrate, 0.013 U NTD, pH 7.5, 25°C, 40 mM (NH4)2SO4, 0.5 mg/ml bovine serum albumin
-
-
r
uracil + deoxyguanosine
deoxyuridine + guanine
-
16.1% conversion after 4 h at standard reaction conditions: 2 mM each substrate, 0.013 U NTD, pH 7.5, 25°C, 40 mM (NH4)2SO4, 0.5 mg/ml bovine serum albumin
-
-
r
uracil + deoxyinosine
deoxyuridine + hypoxanthine
-
23.8% conversion after 4 h at standard reaction conditions: 2 mM each substrate, 0.013 U NTD, pH 7.5, 25°C, 40 mM (NH4)2SO4, 0.5 mg/ml bovine serum albumin
-
-
r
uracil + thymidine
deoxyuridine + thymine
-
43.0% conversion after 4 h at standard reaction conditions: 2 mM each substrate, 0.013 U NTD, pH 7.5, 25°C, 40 mM (NH4)2SO4, 0.5 mg/ml bovine serum albumin
-
-
r
2',3'-dideoxyadenosine + 2,6-diaminopurine
2,6-diaminopurine-2',3'-dideoxyriboside + adenine
-
-
-
?
2',3'-dideoxyadenosine + 2,6-diaminopurine
2,6-diaminopurine-2',3'-dideoxyriboside + adenine
-
-
-
?
2',3'-dideoxyadenosine + 2-fluoroadenine
2-fluoro-2',3'-dideoxyadenosine + adenine
-
-
-
?
2',3'-dideoxyadenosine + 2-fluoroadenine
2-fluoro-2',3'-dideoxyadenosine + adenine
-
-
-
?
2',3'-dideoxyadenosine + 6-chloropurine
6-chloropurine-2',3'-dideoxyriboside + adenine
-
-
-
?
2',3'-dideoxyadenosine + 6-chloropurine
6-chloropurine-2',3'-dideoxyriboside + adenine
-
-
-
?
2',3'-dideoxyadenosine + cytosine
2',3'-dideoxycytidine + adenine
-
-
-
?
2',3'-dideoxyadenosine + cytosine
2',3'-dideoxycytidine + adenine
-
-
-
?
2',3'-dideoxyadenosine + cytosine
2',3'-dideoxycytidine + adenine
-
-
-
?
2',3'-dideoxyadenosine + guanine
2',3'-dideoxyguanosine + adenine
-
-
-
?
2',3'-dideoxyadenosine + guanine
2',3'-dideoxyguanosine + adenine
-
-
-
?
2',3'-dideoxyadenosine + guanine
2',3'-dideoxyguanosine + adenine
-
-
-
?
2',3'-dideoxyadenosine + guanine
2',3'-dideoxyguanosine + adenine
-
-
-
?
2',3'-dideoxyadenosine + hypoxanthine
2',3'-dideoxyinosine + adenine
-
-
-
?
2',3'-dideoxyadenosine + hypoxanthine
2',3'-dideoxyinosine + adenine
high activity
-
-
?
2',3'-dideoxyadenosine + hypoxanthine
2',3'-dideoxyinosine + adenine
Leishmania mexicana 2001
high activity
-
-
?
2',3'-dideoxyadenosine + hypoxanthine
2',3'-dideoxyinosine + adenine
high activity
-
-
?
2',3'-dideoxyadenosine + hypoxanthine
2',3'-dideoxyinosine + adenine
Leishmania mexicana MHOM
high activity
-
-
?
2',3'-dideoxyadenosine + hypoxanthine
2',3'-dideoxyinosine + adenine
Leishmania mexicana U1103
high activity
-
-
?
2',3'-dideoxycytidine + adenine
2',3'-dideoxyadenosine + cytosine
-
-
-
?
2',3'-dideoxycytidine + adenine
2',3'-dideoxyadenosine + cytosine
-
-
-
?
2',3'-dideoxycytidine + adenine
2',3'-dideoxyadenosine + cytosine
-
-
-
?
2',3'-dideoxycytidine + hypoxanthine
2',3'-dideoxyinosine + cytosine
-
-
-
?
2',3'-dideoxycytidine + hypoxanthine
2',3'-dideoxyinosine + cytosine
-
-
-
?
2',3'-dideoxycytidine + hypoxanthine
2',3'-dideoxyinosine + cytosine
-
-
-
?
2',3'-dideoxyguanosine + thymine
2',3'-dideoxythymidine + guanine
-
-
-
?
2',3'-dideoxyguanosine + thymine
2',3'-dideoxythymidine + guanine
-
-
-
?
2',3'-dideoxyguanosine + thymine
2',3'-dideoxythymidine + guanine
-
-
-
?
2',3'-dideoxyinosine + adenine
2',3'-dideoxyadenosine + hypoxanthine
-
-
-
?
2',3'-dideoxyinosine + adenine
2',3'-dideoxyadenosine + hypoxanthine
-
-
-
?
2',3'-dideoxyinosine + adenine
2',3'-dideoxyadenosine + hypoxanthine
-
-
-
?
2',3'-dideoxyinosine + adenine
2',3'-dideoxyadenosine + hypoxanthine
-
-
-
?
2',3'-dideoxyinosine + adenine
2',3'-dideoxyadenosine + hypoxanthine
Leishmania mexicana 2001
-
-
-
?
2',3'-dideoxyinosine + adenine
2',3'-dideoxyadenosine + hypoxanthine
-
-
-
?
2',3'-dideoxyinosine + adenine
2',3'-dideoxyadenosine + hypoxanthine
Leishmania mexicana MHOM
-
-
-
?
2',3'-dideoxyinosine + adenine
2',3'-dideoxyadenosine + hypoxanthine
Leishmania mexicana U1103
-
-
-
?
2',3'-dideoxyinosine + adenine
2',3'-dideoxyadenosine + hypoxanthine
-
-
-
?
2',3'-dideoxyinosine + adenine
2',3'-dideoxyadenosine + hypoxanthine
-
-
-
?
2',3'-dideoxyinosine + guanine
2',3'-dideoxyguanosine + hypoxanthine
-
-
-
?
2',3'-dideoxyinosine + guanine
2',3'-dideoxyguanosine + hypoxanthine
low activity
-
-
?
2',3'-dideoxyinosine + guanine
2',3'-dideoxyguanosine + hypoxanthine
Leishmania mexicana 2001
low activity
-
-
?
2',3'-dideoxyinosine + guanine
2',3'-dideoxyguanosine + hypoxanthine
low activity
-
-
?
2',3'-dideoxyinosine + guanine
2',3'-dideoxyguanosine + hypoxanthine
Leishmania mexicana MHOM
low activity
-
-
?
2',3'-dideoxyinosine + guanine
2',3'-dideoxyguanosine + hypoxanthine
Leishmania mexicana U1103
low activity
-
-
?
2',3'-dideoxythymidine + adenine
2',3'-dideoxyadenosine + thymine
-
-
-
?
2',3'-dideoxythymidine + adenine
2',3'-dideoxyadenosine + thymine
-
-
-
?
2',3'-dideoxythymidine + adenine
2',3'-dideoxyadenosine + thymine
-
-
-
?
2',3'-dideoxyuridine + cytosine
2',3'-dideoxycytidine + uracil
-
-
-
?
2',3'-dideoxyuridine + cytosine
2',3'-dideoxycytidine + uracil
-
-
-
?
2',3'didehydro-2',3'-dideoxythymidine + adenine
2',3'didehydro-2',3'-dideoxyadenosine + thymine
-
-
-
?
2',3'didehydro-2',3'-dideoxythymidine + adenine
2',3'didehydro-2',3'-dideoxyadenosine + thymine
-
-
-
?
2'-deoxy-D-ribosyl-uracil + adenine
2'-deoxy-D-ribosyl-adenine + uracil
-
-
-
-
r
2'-deoxy-D-ribosyl-uracil + adenine
2'-deoxy-D-ribosyl-adenine + uracil
-
-
-
-
r
2'-deoxy-D-ribosyl-uracil + adenine
2'-deoxy-D-ribosyl-adenine + uracil
-
-
-
-
r
2'-deoxy-D-ribosyl-uracil + adenine
2'-deoxy-D-ribosyl-adenine + uracil
-
-
-
-
r
2'-deoxy-D-ribosyl-uracil + adenine
2'-deoxy-D-ribosyl-adenine + uracil
-
-
-
-
r
2'-deoxy-D-ribosyl-uracil + adenine
2'-deoxy-D-ribosyl-adenine + uracil
Peribacillus psychrosaccharolyticus CECT 4074
-
-
-
-
r
2'-deoxy-D-ribosyl-uracil + adenine
2'-deoxy-D-ribosyl-adenine + uracil
-
-
-
-
r
2'-deoxy-D-ribosyl-uracil + adenine
2'-deoxy-D-ribosyl-adenine + uracil
-
-
-
-
r
2'-deoxyadenosine + 5-chlorouracil
2'-deoxy-D-ribosyl-5-chlorouracil + adenine
-
-
-
-
r
2'-deoxyadenosine + 5-chlorouracil
2'-deoxy-D-ribosyl-5-chlorouracil + adenine
-
-
-
-
r
2'-deoxyadenosine + 5-chlorouracil
2'-deoxy-D-ribosyl-5-chlorouracil + adenine
-
-
-
-
r
2'-deoxyadenosine + 5-chlorouracil
2'-deoxy-D-ribosyl-5-chlorouracil + adenine
-
-
-
-
r
2'-deoxyadenosine + 5-chlorouracil
2'-deoxy-D-ribosyl-5-chlorouracil + adenine
-
-
-
?
2'-deoxyadenosine + 5-chlorouracil
2'-deoxy-D-ribosyl-5-chlorouracil + adenine
-
-
-
-
?
2'-deoxyadenosine + 5-chlorouracil
2'-deoxy-D-ribosyl-5-chlorouracil + adenine
-
-
-
-
r
2'-deoxyadenosine + 5-chlorouracil
2'-deoxy-D-ribosyl-5-chlorouracil + adenine
Peribacillus psychrosaccharolyticus CECT 4074
-
-
-
-
r
2'-deoxyadenosine + 5-chlorouracil
2'-deoxy-D-ribosyl-5-chlorouracil + adenine
-
-
-
-
r
2'-deoxyadenosine + 5-chlorouracil
2'-deoxy-D-ribosyl-5-chlorouracil + adenine
-
-
-
-
r
2'-deoxyadenosine + 5-fluorouracil
2'-deoxy-5-fluorouridine + adenine
-
-
-
-
r
2'-deoxyadenosine + 5-fluorouracil
2'-deoxy-5-fluorouridine + adenine
-
-
-
-
r
2'-deoxyadenosine + 5-fluorouracil
2'-deoxy-5-fluorouridine + adenine
-
-
-
-
r
2'-deoxyadenosine + 5-fluorouracil
2'-deoxy-5-fluorouridine + adenine
-
-
-
-
r
2'-deoxyadenosine + 5-fluorouracil
2'-deoxy-5-fluorouridine + adenine
-
-
-
-
r
2'-deoxyadenosine + 5-fluorouracil
2'-deoxy-5-fluorouridine + adenine
Peribacillus psychrosaccharolyticus CECT 4074
-
-
-
-
r
2'-deoxyadenosine + 5-fluorouracil
2'-deoxy-5-fluorouridine + adenine
-
-
-
-
r
2'-deoxyadenosine + 5-fluorouracil
2'-deoxy-5-fluorouridine + adenine
-
-
-
-
r
2'-deoxyadenosine + cytosine
2'-deoxycytidine + adenine
-
-
-
?
2'-deoxyadenosine + cytosine
2'-deoxycytidine + adenine
-
-
-
?
2'-deoxyadenosine + guanine
2'-deoxyguanosine + adenine
-
-
-
?
2'-deoxycytidine + adenine
2'-deoxyadenosine + cytosine
-
-
-
?
2'-deoxycytidine + adenine
2'-deoxyadenosine + cytosine
-
-
-
?
2'-deoxycytidine + adenine
2'-deoxyadenosine + cytosine
-
-
-
?
2'-deoxycytidine + hypoxanthine
2'-deoxyinosine + cytosine
-
-
-
?
2'-deoxycytidine + hypoxanthine
2'-deoxyinosine + cytosine
-
-
-
?
2'-deoxyguanosine + 2,6-diaminopurine
2,6-diaminopurine-2'deoxyriboside + guanine
-
-
-
?
2'-deoxyguanosine + 2,6-diaminopurine
2,6-diaminopurine-2'deoxyriboside + guanine
-
-
-
?
2'-deoxyguanosine + 2-chloroadenine
2-chloro-2'-deoxyadenosine + guanine
-
-
-
?
2'-deoxyguanosine + 2-chloroadenine
2-chloro-2'-deoxyadenosine + guanine
-
-
-
?
2'-deoxyguanosine + adenine
2'-deoxyadenosine + guanine
-
-
-
?
2'-deoxyguanosine + thymine
2'-deoxythymidine + guanine
-
-
-
?
2'-deoxyguanosine + thymine
2'-deoxythymidine + guanine
-
-
-
?
2'-deoxyinosine + adenine
2'-deoxyadenine + hypoxanthine
-
-
-
?
2'-deoxyinosine + adenine
2'-deoxyadenine + hypoxanthine
-
-
-
?
2'-deoxyinosine + adenine
2'-deoxyadenine + hypoxanthine
-
-
-
?
2'-deoxyinosine + adenine
2'-deoxyadenosine + hypoxanthine
-
-
-
?
2'-deoxyinosine + adenine
2'-deoxyadenosine + hypoxanthine
-
-
-
?
2'-deoxyinosine + adenine
2'-deoxyadenosine + hypoxanthine
-
-
-
?
2'-deoxyinosine + adenine
2'-deoxyadenosine + hypoxanthine
-
-
-
?
2'-deoxyinosine + guanine
2'-deoxyguanosine + hypoxanthine
-
-
-
?
2'-deoxyinosine + guanine
2'-deoxyguanosine + hypoxanthine
-
-
-
?
2'-deoxythymidine + 5-fluorouracil
5-fluoro-2'-deoxyuridine + thymine
-
-
-
-
?
2'-deoxythymidine + 5-fluorouracil
5-fluoro-2'-deoxyuridine + thymine
-
-
-
-
?
2'-deoxythymidine + adenine
2'-deoxyadenosine + thymine
-
-
-
?
2'-deoxythymidine + adenine
2'-deoxyadenosine + thymine
-
-
-
?
2'-deoxyuridine + 2,6-diaminopurine
2,6-diaminopurine 2-deoxy-D-ribose 5-phosphate + uracil
-
-
-
?
2'-deoxyuridine + 2,6-diaminopurine
2,6-diaminopurine 2-deoxy-D-ribose 5-phosphate + uracil
-
-
-
-
?
2'-deoxyuridine + 2,6-diaminopurine
2,6-diaminopurine-2'-deoxyriboside + uracil
-
-
-
?
2'-deoxyuridine + 2,6-diaminopurine
2,6-diaminopurine-2'-deoxyriboside + uracil
-
-
-
?
2'-deoxyuridine + 5-azacytosine
5-aza-2'-deoxycytidine + uracil
-
-
-
?
2'-deoxyuridine + 5-azacytosine
5-aza-2'-deoxycytidine + uracil
-
-
-
?
2'-deoxyuridine + 5-azacytosine
5-aza-2'-deoxycytidine + uracil
-
-
-
?
2'-deoxyuridine + 5-fluorocytosine
2'-deoxy-5-fluorocytidine + uracil
-
-
-
?
2'-deoxyuridine + 5-fluorocytosine
2'-deoxy-5-fluorocytidine + uracil
-
-
-
-
?
2'-deoxyuridine + 5-fluorocytosine
5-fluoro-2'-deoxycytidine + uracil
-
-
-
?
2'-deoxyuridine + 5-fluorocytosine
5-fluoro-2'-deoxycytidine + uracil
-
-
-
?
2'-deoxyuridine + 5-trifluorothymine
5-trifluorothymidine + uracil
-
-
-
-
?
2'-deoxyuridine + 5-trifluorothymine
5-trifluorothymidine + uracil
Peribacillus psychrosaccharolyticus CECT 4074
-
-
-
-
?
2'-deoxyuridine + 6-mercaptopurine
6-mercaptopurine 2-deoxy-D-ribose 5-phosphate + uracil
-
-
-
?
2'-deoxyuridine + 6-mercaptopurine
6-mercaptopurine 2-deoxy-D-ribose 5-phosphate + uracil
-
-
-
-
?
2'-deoxyuridine + adenine
2'-deoxyadenosine + uracil
-
-
-
?
2'-deoxyuridine + adenine
2'-deoxyadenosine + uracil
-
-
-
-
?
2'-deoxyuridine + adenine
2'-deoxyadenosine + uracil
Peribacillus psychrosaccharolyticus CECT 4074
-
-
-
-
?
2'-deoxyuridine + adenine
uracil + 2'-deoxyadenosine
immobilized recombinant enzyme, high activity
-
-
?
2'-deoxyuridine + adenine
uracil + 2'-deoxyadenosine
Limosilactobacillus reuteri ATCC 55730
immobilized recombinant enzyme, high activity
-
-
?
2'-deoxyuridine + adenine
uracil + 2'-deoxyadenosine
immobilized recombinant enzyme, high activity
-
-
?
2'-deoxyuridine + benzimidazole
benzimidazole 2-deoxy-D-ribose 5-phosphate + uracil
-
-
-
?
2'-deoxyuridine + benzimidazole
benzimidazole 2-deoxy-D-ribose 5-phosphate + uracil
-
-
-
-
?
2'-deoxyuridine + cytosine
2'-deoxycytidine + uracil
-
-
-
?
2'-deoxyuridine + cytosine
2'-deoxycytidine + uracil
-
-
-
?
2'-deoxyuridine + cytosine
2'-deoxycytidine + uracil
-
-
-
?
2'-deoxyuridine + thymidine
2'-deoxythymidine + uracil
-
-
-
-
?
2'-deoxyuridine + thymidine
2'-deoxythymidine + uracil
Peribacillus psychrosaccharolyticus CECT 4074
-
-
-
-
?
2'-fluoro-2'-deoxyinosine + adenine
2'-fluoro-2'-deoxyadenosine + hypoxanthine
-
-
-
?
2'-fluoro-2'-deoxyinosine + adenine
2'-fluoro-2'-deoxyadenosine + hypoxanthine
high activity
-
-
?
2'-fluoro-2'-deoxyinosine + guanine
2'-fluoro-2'-deoxyguanosine + hypoxanthine
-
-
-
?
2'-fluoro-2'-deoxyinosine + guanine
2'-fluoro-2'-deoxyguanosine + hypoxanthine
high activity
-
-
?
2-deoxy-D-ribosyl-base1 + base2
2-deoxy-D-ribosyl-base2 + base1
-
-
-
?
2-deoxy-D-ribosyl-base1 + base2
2-deoxy-D-ribosyl-base2 + base1
-
-
-
?
2-deoxy-D-ribosyl-base1 + base2
2-deoxy-D-ribosyl-base2 + base1
-
-
-
-
?
2-deoxy-D-ribosyl-base1 + base2
2-deoxy-D-ribosyl-base2 + base1
-
-
-
?
2-deoxy-D-ribosyl-base1 + base2
2-deoxy-D-ribosyl-base2 + base1
-
-
-
?
2-deoxy-D-ribosyl-base1 + base2
2-deoxy-D-ribosyl-base2 + base1
-
-
-
?
2-deoxy-D-ribosyl-base1 + base2
2-deoxy-D-ribosyl-base2 + base1
-
-
-
?
2-deoxy-D-ribosyl-base1 + base2
2-deoxy-D-ribosyl-base2 + base1
-
-
-
-
?
2-deoxy-D-ribosyl-base1 + base2
2-deoxy-D-ribosyl-base2 + base1
-
-
-
-
?
2-deoxy-D-ribosyl-base1 + base2
2-deoxy-D-ribosyl-base2 + base1
-
-
-
?
2-deoxy-D-ribosyl-base1 + base2
2-deoxy-D-ribosyl-base2 + base1
Limosilactobacillus reuteri ATCC 55730
-
-
-
?
2-deoxy-D-ribosyl-base1 + base2
2-deoxy-D-ribosyl-base2 + base1
-
-
-
?
2-deoxy-D-ribosyl-base1 + base2
2-deoxy-D-ribosyl-base2 + base1
-
-
-
-
?
2-deoxy-D-ribosyl-base1 + base2
2-deoxy-D-ribosyl-base2 + base1
Peribacillus psychrosaccharolyticus CECT 4074
-
-
-
-
?
2-deoxy-D-ribosyl-base1 + base2
2-deoxy-D-ribosyl-base2 + base1
-
-
-
?
2-deoxy-D-ribosyl-base1 + base2
2-deoxy-D-ribosyl-base2 + base1
-
-
-
?
5-fluorouracil + 2'-deoxyadenosine
5-fluoro-2'-deoxyuridine + adenine
immobilized recombinant enzyme
-
-
?
5-fluorouracil + 2'-deoxyadenosine
5-fluoro-2'-deoxyuridine + adenine
Limosilactobacillus reuteri ATCC 55730
immobilized recombinant enzyme
-
-
?
5-fluorouracil + 2'-deoxyadenosine
5-fluoro-2'-deoxyuridine + adenine
immobilized recombinant enzyme
-
-
?
5-fluorouracil + 2'-deoxyuridine
5-fluoro-2'-deoxyuridine + uracil
immobilized recombinant enzyme
-
-
?
5-fluorouracil + 2'-deoxyuridine
5-fluoro-2'-deoxyuridine + uracil
Limosilactobacillus reuteri ATCC 55730
immobilized recombinant enzyme
-
-
?
5-fluorouracil + 2'-deoxyuridine
5-fluoro-2'-deoxyuridine + uracil
immobilized recombinant enzyme
-
-
?
9-beta-D-arabinofuranosyl adenine + hypoxanthine
?
-
-
-
?
9-beta-D-arabinofuranosyl adenine + hypoxanthine
?
low activity
-
-
?
9-beta-D-arabinofuranosyl hypoxanthine + adenine
?
-
-
-
?
9-beta-D-arabinofuranosyl hypoxanthine + guanine
?
-
-
-
?
9-beta-D-arabinofuranosyl hypoxanthine + guanine
?
low activity
-
-
?
cytosine + thymidine
deoxycytidine + thymine
-
53.5% conversion after 4 h at standard reaction conditions: 2 mM each substrate, 0.013 U NTD, pH 7.5, 25°C, 40 mM (NH4)2SO4, 0.5 mg/ml bovine serum albumin
-
-
r
cytosine + thymidine
deoxycytidine + thymine
-
standard reaction for cofactor analyses as well as activating compound and inhibitor studies, in presence of 40 mM ammonium sulfate and 0.5 mg/ml bovine serum albumin
-
-
r
deoxyadenosine + cytosine
adenine + deoxycytidine
-
DRTase II
-
-
?
deoxyadenosine + hypoxanthine
adenine + deoxyinosine + 7-(beta-D-2'-deoxyribufuranosyl)hypoxanthine
-
-
-
?
deoxyadenosine + hypoxanthine
adenine + deoxyinosine + 7-(beta-D-2'-deoxyribufuranosyl)hypoxanthine
-
-
-
-
r
deoxycytidine + adenine
cytosine + deoxyadenosine
-
-
-
-
r
deoxyinosine + adenine
hypoxanthine + deoxyadenosine
-
-
-
-
r
deoxyinosine + adenine
hypoxanthine + deoxyadenosine
-
DRTase I
-
-
r
deoxyinosine + cytosine
hypoxanthine + deoxycytidine
-
DRTase II
-
-
?
deoxyinosine + guanine
hypoxanthine + deoxyguanosine
-
DRTase I
-
-
?
thymidine + adenine
2'-deoxy-D-ribosyl-adenine + thymine
-
-
-
-
r
thymidine + adenine
2'-deoxy-D-ribosyl-adenine + thymine
-
-
-
-
r
thymidine + adenine
2'-deoxy-D-ribosyl-adenine + thymine
-
-
-
-
r
thymidine + adenine
2'-deoxy-D-ribosyl-adenine + thymine
Peribacillus psychrosaccharolyticus CECT 4074
-
-
-
-
r
thymidine + adenine
2'-deoxy-D-ribosyl-adenine + thymine
-
-
-
-
r
thymidine + adenine
2'-deoxyadenosine + thymine
Limosilactobacillus fermentum CGMCC 1.2133
-
-
-
-
?
thymidine + adenine
thymine + adenosine
-
-
3-deoxyribonucleoside
?
thymidine + cytosine
thymine + 2'-deoxycytidine
-
-
-
-
?
thymidine + guanine
thymine + 2'-deoxyguanosine
-
42.3% of substrate converted into deoxynucleoside after 2 h
-
-
?
additional information
?
-
-
the enzyme is selective for 6-aminopurine compared to 6-oxopurines and for 2'-deoxyuridine compared to thymidine
-
-
?
additional information
?
-
-
the enzyme is selective for 6-aminopurine compared to 6-oxopurines and for 2'-deoxyuridine compared to thymidine
-
-
?
additional information
?
-
CtNDT recognizes purine bases and their corresponding 2'-deoxynucleosides but is also proficient using cytosine and 2'-deoxycytidine as substrates
-
-
-
additional information
?
-
-
CtNDT recognizes purine bases and their corresponding 2'-deoxynucleosides but is also proficient using cytosine and 2'-deoxycytidine as substrates
-
-
-
additional information
?
-
CtNDT recognizes purine bases and their corresponding 2'-deoxynucleosides but is also proficient using cytosine and 2'-deoxycytidine as substrates, substrate specificity, overview. Cytidine does not react with 2',3'-dideoxyinosine, 2'-fluoro-2'-deoxyinosine, 9-beta-D-arabinofuranosyl guanine, and 9-beta-D-arabinofuranosyl hypoxanthine
-
-
-
additional information
?
-
-
CtNDT recognizes purine bases and their corresponding 2'-deoxynucleosides but is also proficient using cytosine and 2'-deoxycytidine as substrates, substrate specificity, overview. Cytidine does not react with 2',3'-dideoxyinosine, 2'-fluoro-2'-deoxyinosine, 9-beta-D-arabinofuranosyl guanine, and 9-beta-D-arabinofuranosyl hypoxanthine
-
-
-
additional information
?
-
CtNDT recognizes purine bases and their corresponding 2'-deoxynucleosides but is also proficient using cytosine and 2'-deoxycytidine as substrates
-
-
-
additional information
?
-
-
CtNDT recognizes purine bases and their corresponding 2'-deoxynucleosides but is also proficient using cytosine and 2'-deoxycytidine as substrates
-
-
-
additional information
?
-
CtNDT recognizes purine bases and their corresponding 2'-deoxynucleosides but is also proficient using cytosine and 2'-deoxycytidine as substrates, substrate specificity, overview. Cytidine does not react with 2',3'-dideoxyinosine, 2'-fluoro-2'-deoxyinosine, 9-beta-D-arabinofuranosyl guanine, and 9-beta-D-arabinofuranosyl hypoxanthine
-
-
-
additional information
?
-
-
CtNDT recognizes purine bases and their corresponding 2'-deoxynucleosides but is also proficient using cytosine and 2'-deoxycytidine as substrates, substrate specificity, overview. Cytidine does not react with 2',3'-dideoxyinosine, 2'-fluoro-2'-deoxyinosine, 9-beta-D-arabinofuranosyl guanine, and 9-beta-D-arabinofuranosyl hypoxanthine
-
-
-
additional information
?
-
-
nucleoside deoxyribosyltransferase I is strictly specific for transfer between purine bases. Nucleoside deoxyribosyltransferase II catalyzes the transfer of the deoxyribosyl moiety between purines or pyrimidines as well as from a purine to a pyrimidine
-
-
?
additional information
?
-
DRTase I is strictly specific for transfer between purine bases. DRTase II catalyzes the transfer of the deoxyribosyl moiety between purines or pyrimidines as well as from a purine to a pyrimidine
-
-
?
additional information
?
-
-
DRTase I is strictly specific for transfer between purine bases. DRTase II catalyzes the transfer of the deoxyribosyl moiety between purines or pyrimidines as well as from a purine to a pyrimidine
-
-
?
additional information
?
-
DRTase I is specific for purines with preference for deoxyinosine, deoxyadenosine, deoxyguanosine as donor substrates
-
-
?
additional information
?
-
-
DRTase I is specific for purines with preference for deoxyinosine, deoxyadenosine, deoxyguanosine as donor substrates
-
-
?
additional information
?
-
specific role for DRTase I in the metabolism of deoxyinosine
-
-
?
additional information
?
-
-
specific role for DRTase I in the metabolism of deoxyinosine
-
-
?
additional information
?
-
NDT is highly specific for 20-deoxynucleoside because Glu98 of NDT forms a hydrogen bond with 3'-OH of 2'-deoxynucleoside. When the sugar donor is a ribonucleoside, Glu98 forms a hydrogen bond with 2'-OH of ribose and the enzyme is inactive. Usage of an indophenol colorimetric assay
-
-
-
additional information
?
-
-
NDT is highly specific for 20-deoxynucleoside because Glu98 of NDT forms a hydrogen bond with 3'-OH of 2'-deoxynucleoside. When the sugar donor is a ribonucleoside, Glu98 forms a hydrogen bond with 2'-OH of ribose and the enzyme is inactive. Usage of an indophenol colorimetric assay
-
-
-
additional information
?
-
-
DRTase I is strictly specific for transfer between purine bases. DRTase II catalyzes the transfer of the deoxyribosyl moiety between purines or pyrimidines as well as from a purine to a pyrimidine
-
-
?
additional information
?
-
no activity with 3'-azido-3'-deoxythymidine or 3'-amino-3'-deoxythymidine with adenine as acceptor
-
-
?
additional information
?
-
the enzyme accepts purines or pyrimidines as donor substrates, showing preference for 2'-deoxycytidine > 2'-deoxyuridine > 2'-deoxyadenosine and thymidine > 2'-deoxyguanosine >>> 2'-deoxyinosine, and a similar preference for pyrimidines and purines
-
-
?
additional information
?
-
-
the enzyme participates in the nucleoside salvage pathway
-
-
?
additional information
?
-
-
the enzyme is selective for 6-aminopurine compared to 6-oxopurines and for 2'-deoxyuridine compared to thymidine
-
-
?
additional information
?
-
-
the enzyme is selective for 6-aminopurine compared to 6-oxopurines and for 2'-deoxyuridine compared to thymidine
-
-
?
additional information
?
-
-
preferably purine 2-deoxyribonucleoside synthesis compared to pyrimidine deoxyribonucleoside synthesis although both are catalysed
-
-
?
additional information
?
-
-
ribonucleoside, arabinosylnucleoside and 2,3-dideoxyribonucleoside are no substrates for base-exchange by YejD
-
-
?
additional information
?
-
-
no activity with 3'-azido-3'-deoxythymidine or 3'-amino-3'-deoxythymidine with adenine as acceptor
-
-
?
additional information
?
-
-
the enzyme accepts purines or pyrimidines as donor substrates, showing preference for 2'-deoxycytidine > 2'-deoxyuridine > 2'-deoxyadenosine and thymidine > 2'-deoxyguanosine >>> 2'-deoxyinosine, and a similar preference for pyrimidines and purines
-
-
?
additional information
?
-
NDT can directly transfer 2'-deoxyribosyl moiety from a 2'-deoxynucleoside donor to a nucleobase acceptor. LhNDT does not only catalyze the transfer between purines but also the transfer between pyrimidines
-
-
-
additional information
?
-
-
NDT can directly transfer 2'-deoxyribosyl moiety from a 2'-deoxynucleoside donor to a nucleobase acceptor. LhNDT does not only catalyze the transfer between purines but also the transfer between pyrimidines
-
-
-
additional information
?
-
NDT can directly transfer 2'-deoxyribosyl moiety from a 2'-deoxynucleoside donor to a nucleobase acceptor. LhNDT does not only catalyze the transfer between purines but also the transfer between pyrimidines
-
-
-
additional information
?
-
-
the DEAE-Sepharose-immobilized enzyme is used to obtain 5-fluorouracil-2'-deoxyriboside (floxuridine), 6-chloropurine-2'-deoxyriboside, 6-bromopurine-2'-deoxyriboside, 6-chloro-2-fluoropurine-2'-deoxyriboside, 5-bromo-2'-deoxyuridine, and 5-chloro-2'-deoxyuridine
-
-
?
additional information
?
-
-
the recombinant enzyme exhibits hydrolytic activity for both purine and pyrimidine nucleosides
-
-
-
additional information
?
-
-
the recombinant enzyme exhibits hydrolytic activity for both purine and pyrimidine nucleosides
-
-
-
additional information
?
-
-
the enzyme glycosylates N2,3-ethenoguanine (8,9-dihydro-9-oxoimidazo[2,1-b]purine) at N7 and N1 with somewhat less selectivity for N7
-
-
?
additional information
?
-
enzyme does not interchange bases between ribonucleosides, but is able to catalyze the transfer of 2'-deoxyribose between purine or pyrimidine and/or pyrimidine bases. The enzyme prefers pyrimidines to purines as substrates, dUrd being the best nucleoside donor, followed by dThd, dAdo, and dIno, whereas cytosine is the best base acceptor and hypoxanthine is the worst
-
-
?
additional information
?
-
-
enzyme does not interchange bases between ribonucleosides, but is able to catalyze the transfer of 2'-deoxyribose between purine or pyrimidine and/or pyrimidine bases. The enzyme prefers pyrimidines to purines as substrates, dUrd being the best nucleoside donor, followed by dThd, dAdo, and dIno, whereas cytosine is the best base acceptor and hypoxanthine is the worst
-
-
?
additional information
?
-
arabinosyluracil is a poor substrate for the immobilized recombinant enzyme
-
-
-
additional information
?
-
-
arabinosyluracil is a poor substrate for the immobilized recombinant enzyme
-
-
-
additional information
?
-
Limosilactobacillus reuteri ATCC 55730
arabinosyluracil is a poor substrate for the immobilized recombinant enzyme
-
-
-
additional information
?
-
arabinosyluracil is a poor substrate for the immobilized recombinant enzyme
-
-
-
additional information
?
-
-
the enzyme is selective for 6-aminopurine compared to 6-oxopurines and for 2'-deoxyuridine compared to thymidine
-
-
?
additional information
?
-
-
the enzyme is not able to interchange the deoxyribosyl moiety between ribonucleosides and is free of nucleoside phosphorylases traces
-
-
?
additional information
?
-
Peribacillus psychrosaccharolyticus CECT 4074
-
the enzyme is not able to interchange the deoxyribosyl moiety between ribonucleosides and is free of nucleoside phosphorylases traces
-
-
?
additional information
?
-
Peribacillus psychrosaccharolyticus CECT 4074
-
the enzyme is selective for 6-aminopurine compared to 6-oxopurines and for 2'-deoxyuridine compared to thymidine
-
-
?
additional information
?
-
-
the enzyme is selective for 6-aminopurine compared to 6-oxopurines and for 2'-deoxyuridine compared to thymidine
-
-
?
additional information
?
-
-
the enzyme is selective for 6-aminopurine compared to 6-oxopurines and for 2'-deoxyuridine compared to thymidine
-
-
?
additional information
?
-
-
RCL cleaves the N-glycosidic bond of deoxynucleoside 5'-monophosphates to yield two reaction products, deoxyribose 5-phosphate and purine or pyrimidine base
-
-
?
additional information
?
-
-
the enzyme is important in the salvage pathway of nucleoside recycling, Trypanosoma brucei lacks enzymes of de novo purine biosynthesis
-
-
?
additional information
?
-
no activity with theophylline, and with 2',3'-dideoxyadenosine/2-chloroadenine, or 2',3'-dideoxyinosine/2-fluoroadenine, or 9-beta-D-arabinofuranosyl adenine/6-mercaptopurine
-
-
-
additional information
?
-
no activity with xanthine, 7-deazaxanthine, and 7-deazahypoxanthine, substrate specificity, overview. No significant preference exists between 6-aminopurines and 6-oxopurines as base acceptors. Binding structures of inosine within the active site of wild-type TbPDT and TbPDTY5F
-
-
-
additional information
?
-
no activity with xanthine, 7-deazaxanthine, and 7-deazahypoxanthine, substrate specificity, overview. No significant preference exists between 6-aminopurines and 6-oxopurines as base acceptors. Binding structures of inosine within the active site of wild-type TbPDT and TbPDTY5F
-
-
-
additional information
?
-
no activity with theophylline, and with 2',3'-dideoxyadenosine/2-chloroadenine, or 2',3'-dideoxyinosine/2-fluoroadenine, or 9-beta-D-arabinofuranosyl adenine/6-mercaptopurine
-
-
-
thymidine + 2-hydroxy-6-mercaptopurine
additional information
-
-
-
2 products
r
thymidine + 2-mercaptoadenine
additional information
-
-
-
2 products
r
thymidine + 2-mercaptopurine
additional information
-
-
-
2 products
r
thymidine + 6-hydrazinepurine
additional information
-
-
-
several products observed
r
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NATURAL SUBSTRATE
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate)
(Substrate)
LITERATURE
(Substrate)
(Substrate)
COMMENTARY
(Product)
(Product)
LITERATURE
(Product)
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
r=reversible
ir=irreversible
?=not specified
purine base + 2-deoxy-D-ribosyl-base
purine deoxyribonucleoside + base
-
-
preferred over pyrimidine deoxyribonucleoside synthesis
-
r
pyrimidine base + 2-deoxy-D-ribosyl-base
pyrimidine deoxyribonucleoside + base
-
-
-
-
r
2'-deoxy-D-ribosyl-uracil + adenine
2'-deoxy-D-ribosyl-adenine + uracil
-
-
-
-
r
2'-deoxy-D-ribosyl-uracil + adenine
2'-deoxy-D-ribosyl-adenine + uracil
-
-
-
-
r
2'-deoxy-D-ribosyl-uracil + adenine
2'-deoxy-D-ribosyl-adenine + uracil
-
-
-
-
r
2'-deoxy-D-ribosyl-uracil + adenine
2'-deoxy-D-ribosyl-adenine + uracil
-
-
-
-
r
2'-deoxy-D-ribosyl-uracil + adenine
2'-deoxy-D-ribosyl-adenine + uracil
-
-
-
-
r
2'-deoxy-D-ribosyl-uracil + adenine
2'-deoxy-D-ribosyl-adenine + uracil
Peribacillus psychrosaccharolyticus CECT 4074
-
-
-
-
r
2'-deoxy-D-ribosyl-uracil + adenine
2'-deoxy-D-ribosyl-adenine + uracil
-
-
-
-
r
2'-deoxy-D-ribosyl-uracil + adenine
2'-deoxy-D-ribosyl-adenine + uracil
-
-
-
-
r
2-deoxy-D-ribosyl-base1 + base2
2-deoxy-D-ribosyl-base2 + base1
-
-
-
?
2-deoxy-D-ribosyl-base1 + base2
2-deoxy-D-ribosyl-base2 + base1
-
-
-
?
2-deoxy-D-ribosyl-base1 + base2
2-deoxy-D-ribosyl-base2 + base1
-
-
-
-
?
2-deoxy-D-ribosyl-base1 + base2
2-deoxy-D-ribosyl-base2 + base1
-
-
-
?
2-deoxy-D-ribosyl-base1 + base2
2-deoxy-D-ribosyl-base2 + base1
-
-
-
?
2-deoxy-D-ribosyl-base1 + base2
2-deoxy-D-ribosyl-base2 + base1
-
-
-
?
2-deoxy-D-ribosyl-base1 + base2
2-deoxy-D-ribosyl-base2 + base1
-
-
-
?
2-deoxy-D-ribosyl-base1 + base2
2-deoxy-D-ribosyl-base2 + base1
-
-
-
-
?
2-deoxy-D-ribosyl-base1 + base2
2-deoxy-D-ribosyl-base2 + base1
-
-
-
-
?
2-deoxy-D-ribosyl-base1 + base2
2-deoxy-D-ribosyl-base2 + base1
-
-
-
?
2-deoxy-D-ribosyl-base1 + base2
2-deoxy-D-ribosyl-base2 + base1
Limosilactobacillus reuteri ATCC 55730
-
-
-
?
2-deoxy-D-ribosyl-base1 + base2
2-deoxy-D-ribosyl-base2 + base1
-
-
-
?
2-deoxy-D-ribosyl-base1 + base2
2-deoxy-D-ribosyl-base2 + base1
-
-
-
-
?
2-deoxy-D-ribosyl-base1 + base2
2-deoxy-D-ribosyl-base2 + base1
Peribacillus psychrosaccharolyticus CECT 4074
-
-
-
-
?
2-deoxy-D-ribosyl-base1 + base2
2-deoxy-D-ribosyl-base2 + base1
-
-
-
?
2-deoxy-D-ribosyl-base1 + base2
2-deoxy-D-ribosyl-base2 + base1
-
-
-
?
additional information
?
-
CtNDT recognizes purine bases and their corresponding 2'-deoxynucleosides but is also proficient using cytosine and 2'-deoxycytidine as substrates
-
-
-
additional information
?
-
-
CtNDT recognizes purine bases and their corresponding 2'-deoxynucleosides but is also proficient using cytosine and 2'-deoxycytidine as substrates
-
-
-
additional information
?
-
CtNDT recognizes purine bases and their corresponding 2'-deoxynucleosides but is also proficient using cytosine and 2'-deoxycytidine as substrates
-
-
-
additional information
?
-
-
CtNDT recognizes purine bases and their corresponding 2'-deoxynucleosides but is also proficient using cytosine and 2'-deoxycytidine as substrates
-
-
-
additional information
?
-
specific role for DRTase I in the metabolism of deoxyinosine
-
-
?
additional information
?
-
-
specific role for DRTase I in the metabolism of deoxyinosine
-
-
?
additional information
?
-
-
the enzyme participates in the nucleoside salvage pathway
-
-
?
additional information
?
-
-
preferably purine 2-deoxyribonucleoside synthesis compared to pyrimidine deoxyribonucleoside synthesis although both are catalysed
-
-
?
additional information
?
-
-
the enzyme is important in the salvage pathway of nucleoside recycling, Trypanosoma brucei lacks enzymes of de novo purine biosynthesis
-
-
?
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METALS and IONS
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
Ca2+
stabilizes the enzyme 1t 10-20 mM, Ca2+ markedly improves the thermostability of LhNDT, significantly stimulates the LhNDT activity up to 184% at 10 mM
Co2+
significantly stimulates the LhNDT activity up to 146% at 10 mM
K+
-
enhances base exchange activity and thermostability, 1.66-fold activation by 1 mM KCl (reaches plateau at 20 mM)
Mn2+
significantly stimulates the LhNDT activity up to 127% at 10 mM
NH4+
-
enhances base exchange activity and thermostability, 1.43-fold activation by 1 mM (NH4)2SO4 (reaches plateau at 20 mM)
Ni2+
significantly stimulates the LhNDT activity up to 138% at 10 mM
Zn2+
significantly stimulates the LhNDT activity up to 124% at 10 mM
additional information
additional information
-
enzymatic activity depends on monovalent cations as cofactors rather than catalysts
additional information
no significant changes are observed with Ba2+ and Cr3+
additional information
-
no significant changes are observed with Ba2+ and Cr3+
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INHIBITOR
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
amino-pyrrolo-pyrimidine
-
12.1 mM, complete inhibition of the reaction with adenine and deoxycytidine
Hydroxypyrrolopyrimidine
-
0.33 mM, 66% inhibition of the reaction with adenine and deoxycytidine
imidazole
-
12.1 mM, 76% inhibition of the reaction with adenine and deoxycytidine
deoxyadenosine
-
linear competitive inhibition of deoxyinosine and linear non-competitive inhibition of adenine
Tris
-
complete inhibition of deoxyribosyltransferase I at 0.1 M, pH 6.9. 62% inhibition of nucleoside deoxyribosyltransferase I at 0.8 M, pH 5.9
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ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
K+
-
K+-activated type II enzyme: binding of K+ is required to enhance activity through induced conformational change
additional information
-
does not require phosphate derivatives such as ATP, thiamine diphosphate, pyridoxal phosphate to complete 2-deoxyribose transfer
-
additional information
no activation observed with NH4+, K+ or Rb+
-
additional information
-
no activation observed with NH4+, K+ or Rb+
-
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DISEASE
TITLE OF PUBLICATION
LINK TO PUBMED
Neoplasms
Deoxynucleoside 5-monophosphate N-glycosidase from a phylogenetically distant metazoa, sponge.
Neoplasms
Molecular Basis of NDT-Mediated Activation of Nucleoside-Based Prodrugs and Application in Suicide Gene Therapy.
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KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
0.2
2'-deoxyinosine
pH 6.0, 50°C, recombinant wild-type enzyme, transglycosylation, with adenine
0.7
2'-deoxyinosine
pH 6.0, 50°C, recombinant wild-type enzyme, nucleobase release
1.2
2'-deoxyinosine
pH 6.0, 50°C, recombinant mutant V11A, transglycosylation, with adenine
2
2'-deoxyinosine
pH 6.0, 50°C, recombinant mutant V11S, transglycosylation, with adenine
5.1
2'-deoxyinosine
pH 6.0, 50°C, recombinant mutant V11A, nucleobase release
6.8
2'-deoxyinosine
pH 6.0, 50°C, recombinant mutant V11S, nucleobase release
1.8
adenine
pH 6.0, 50°C, recombinant wild-type enzyme, transglycosylation, with 2'-deoxyinosine
2.9
adenine
pH 6.0, 50°C, recombinant mutant V11A, transglycosylation, with 2'-deoxyinosine
2.9
adenine
pH 6.0, 50°C, recombinant mutant V11S, transglycosylation, with 2'-deoxyinosine
additional information
additional information
Michaelis-Menten kinetics
-
additional information
additional information
-
Michaelis-Menten kinetics
-
additional information
additional information
Michaelis-Menten kinetics
-
additional information
additional information
-
kinetics and substrate specifiicty
-
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TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
6.4
2'-deoxyinosine
pH 6.0, 50°C, recombinant wild-type enzyme, transglycosylation, with adenine
7.9
2'-deoxyinosine
pH 6.0, 50°C, recombinant wild-type enzyme, nucleobase release
14.3
2'-deoxyinosine
pH 6.0, 50°C, recombinant mutant V11A, transglycosylation, with adenine
17
2'-deoxyinosine
pH 6.0, 50°C, recombinant mutant V11S, transglycosylation, with adenine
39.6
2'-deoxyinosine
pH 6.0, 50°C, recombinant mutant V11A, nucleobase release
40.7
2'-deoxyinosine
pH 6.0, 50°C, recombinant mutant V11S, nucleobase release
10.2
adenine
pH 6.0, 50°C, recombinant wild-type enzyme, transglycosylation, with 2'-deoxyinosine
23.4
adenine
pH 6.0, 50°C, recombinant mutant V11A, transglycosylation, with 2'-deoxyinosine
23.8
adenine
pH 6.0, 50°C, recombinant mutant V11S, transglycosylation, with 2'-deoxyinosine
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
kcat/KM VALUE [1/mMs-1]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
0.192
2'-deoxycytidine
mutant F13R/D92S/N123S, pH 6.0, 37°C
5.99
2'-deoxyinosine
pH 6.0, 50°C, recombinant mutant V11S, nucleobase release
7.77
2'-deoxyinosine
pH 6.0, 50°C, recombinant mutant V11A, nucleobase release
8.5
2'-deoxyinosine
pH 6.0, 50°C, recombinant mutant V11S, transglycosylation, with adenine
11.29
2'-deoxyinosine
pH 6.0, 50°C, recombinant wild-type enzyme, nucleobase release
11.92
2'-deoxyinosine
pH 6.0, 50°C, recombinant mutant V11A, transglycosylation, with adenine
32
2'-deoxyinosine
pH 6.0, 50°C, recombinant wild-type enzyme, transglycosylation, with adenine
5.67
adenine
pH 6.0, 50°C, recombinant wild-type enzyme, transglycosylation, with 2'-deoxyinosine
8.07
adenine
pH 6.0, 50°C, recombinant mutant V11A, transglycosylation, with 2'-deoxyinosine
8.21
adenine
pH 6.0, 50°C, recombinant mutant V11S, transglycosylation, with 2'-deoxyinosine
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Ki VALUE [mM]
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
0.01
6-methylthio-GMP
-
pH 7.5, 37°C, recombinant wild-type enzyme, versus dGMP
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SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
0.01
0.017
wild-type, with 2',3'-dideoxythymidine and adenine as substrates
0.02
mutant G9S, with 2'-deoxyinosine and adenine as substrates
0.03
0.036
0.04
0.05
0.056
wild-type, with 2',3'-dideoxyuridine and cytosine as substrates
0.06
0.1
0.13
purified recombinant wild-type enzyme, pH 6.0, 37°C, substrate 2',3'-dideoxyinosine
0.19
wild-type, with 2',3'-dideoxyuridine and cytosine as substrates
0.2
0.25
mutant G9S, with 3'-dideoxythymidine and adenine as substrates
0.4
mutant G9S, with 2',3'-dideoxyguanosine and thymine as substrates
0.42
mutant A15T, 2',3'-dideoxyadenosine and thymine as substrates
0.46
mutant A15T, 2'-deoxyadenosine and thymine as substrates
0.47
mutant G9S, with 2'-deoxyinosine and adenine as substrates
0.5
0.6
mutant G9S, with 2',3'-dideoxyguanosine and thymine as substrates
0.8
0.86
purified recombinant mutant NDT G10S, pH 6.0, 37°C, substrate 2',3'-dideoxyinosine
0.9
recombinant wild-type enzyme with substrates inosine and adenine, pH 6.0, 50°C
1.04
mutant G9S, with 2'-deoxythymidine and adenine as substrates
1.08
wild-type, with 2'-deoxyinosine and adenine as substrates
1.1
1.25
mutant G9S, with 2'-deoxycytidine and hypoxanthine as substrates
1.29
1.3
mutant G9S, with 2',3'-dideoxyuridine and cytosine as substrates
1.35
mutant A15T, 2',3'-dideoxyadenosine and cytosine as substrates
1.47
mutant A15T, 2',3'-dideoxycytidine and thymine as substrates
1.6
1.9
10.5
purified recombinant wild-type enzyme, pH 6.0, 50°C, substrates 2'-deoxyadenosine and guanine
11
11.1
recombinant mutant Y5F/N53D with substrates 2'-deoxyinosine and adenine, pH 4.0, 50°C
11.3
recombinant mutant Y5F with substrates 2'-deoxyinosine and adenine, pH 6.0, 50°C
11.9
wild-type, with 2'-deoxythymidine and adenine as substrates
12.3
recombinant mutant Y5F with substrates 2'-deoxyinosine and adenine, pH 4.0, 50°C
12.7
mutant G9S, with 2'-deoxyuridine and cytosine as substrates
173
wild-type, with 2'-deoxycytidine and adenine as substrates
2.7
recombinant mutant Y5F with substrates inosine and adenine, pH 6.0, 50°C
20.1
21.4
purified recombinant wild-type enzyme, pH 6.0, 50°C, substrates 2'-deoxyguanosine and adenine
27.1
mutant G9S, with 2'-deoxycytidine and adenine as substrates
29
wild-type, with 2',3'-dideoxycytidine and adenine as substrates
3.1
mutant G9S, with 2'-deoxyuridine and cytosine as substrates
3.6
wild-type, with 2'-deoxyinosine and adenine as substrates
32
wild-type, 2'-deoxycytidine and thymine as substrates
33.2
wild-type, 2'-deoxyadenosine and cytosine as substrates
38.5
39
purified recombinant wild-type enzyme, pH 6.0, 50°C, substrates 2'-deoxyguanosine and hypoxanthine