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Enzyme Nomenclature
Information on EC 2.4.2.6 - nucleoside deoxyribosyltransferase
for references in articles please use BRENDA:EC2.4.2.6
Word Map on EC 2.4.2.6
- 2.4.2.6
-
lactobacillus
-
deoxyribosylation
- helveticus
- deoxyribonucleosides
- leichmannii
- synthesis
- 2'-deoxyuridine
- 2'-deoxyribonucleosides
- 2'-deoxynucleosides
- deoxyadenosine
- reuteri
- medicine
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The expected taxonomic range for this enzyme is: Bacteria, Eukaryota
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EC NUMBER 
COMMENTARY 
2.4.2.6
-
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RECOMMENDED NAME
GeneOntology No.
nucleoside deoxyribosyltransferase
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REACTION
REACTION DIAGRAM
COMMENTARY
ORGANISM
UNIPROT
LITERATURE
2-deoxy-D-ribosyl-base1 + base2 = 2-deoxy-D-ribosyl-base2 + base1
ping-pong bi bi mechanism
-
2-deoxy-D-ribosyl-base1 + base2 = 2-deoxy-D-ribosyl-base2 + base1
Glu105, Asp-99 and Asn-172 are catalytic active site residues
-
2-deoxy-D-ribosyl-base1 + base2 = 2-deoxy-D-ribosyl-base2 + base1
Glu90 is a catalytic active site residue, ligand binding and active site structures, overview
-
2-deoxy-D-ribosyl-base1 + base2 = 2-deoxy-D-ribosyl-base2 + base1
-
-
-
-
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REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
pentosyl group transfer
transglycosylation
pentosyl group transfer
-
-
-
-
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PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
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SYSTEMATIC NAME
IUBMB Comments
nucleoside:purine(pyrimidine) deoxy-D-ribosyltransferase
Base1 and base2 represent various purines and pyrimidines.
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CAS REGISTRY NUMBER
COMMENTARY
9026-86-2
-
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ORGANISM
COMMENTARY
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
nucleoside deoxyribosyltransferase-I and nucleoside deoxyribosyltransferase-I
-
-
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SUBSTRATE
PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate)
(Substrate)
LITERATURE
(Substrate)
(Substrate)
COMMENTARY
(Product)
(Product)
LITERATURE
(Product)
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
r=reversible
ir=irreversible
?=not specified
2',3'-dideoxyadenosine + cytosine
2',3'-dideoxycytidine + adenine
-
-
-
-
?
2',3'-dideoxyadenosine + thymine
2',3'-dideoxythymidine + adenine
-
-
-
-
?
2',3'-dideoxycytidine + thymine
2',3'-dideoxythymidine + cytosine
-
-
-
-
?
2',3'didehydro-2',3'-dideoxythymidine + adenine
2',3'didehydro-2',3'-dideoxyadenosine + thymine
2'-deoxyadenosine + 5-fluorouracil
2'-deoxy-D-ribosyl-5-fluorouracil + adenine
reaction is catalyzed by mutants F13R/D92S/N123S and F13Q/D92S/N123S
-
-
?
2'-deoxycytidine + 2,6-diaminopurine
2,6-diaminopurine 2-deoxy-D-ribose 5-phosphate + cytosine
reaction is catalyzed by mutants F13R/D92S/N123S and F13Q/D92S/N123S
-
-
?
2'-deoxycytidine + 5-bromouracil
2'-deoxy-5-bromouridine + cytosine
reaction is catalyzed by mutants F13R/D92S/N123S and F13Q/D92S/N123S
-
-
?
2'-deoxycytidine + 6-chloroguanine
cytosine + 2'-deoxy-6-chloroguanosine
-
DRTase II
-
-
?
2'-deoxycytidine + 6-chloropurine
6-chloropurine 2-deoxy-D-ribose 5-phosphate + cytosine
reaction is catalyzed by mutants F13R/D92S/N123S and F13Q/D92S/N123S
-
-
?
2'-deoxyuridine + 2,6-diaminopurine
2,6-diaminopurine 2-deoxy-D-ribose 5-phosphate + uracil
2'-deoxyuridine + 5-azacytosine
5-aza-2'-deoxycytidine + uracil
-
-
-
-
?
2'-deoxyuridine + 5-ethyluracil
5-ethyl-2'-deoxyuridine + uracil
-
-
-
-
?
2'-deoxyuridine + 5-methylcytosine
5-methyl-2'-deoxycytidine + uracil
-
-
-
-
?
5'-fluoro-5'-thymidine + adenine
5'-fluoro-5'-deoxythymine + adenosine
-
deoxyribosyltransferase II
-
-
?
adenine + deoxycytidine
deoxyadenosine + cytosine
-
64.0% conversion after 4 h at standard reaction conditions: 2 mM each substrate, 0.013 U NTD, pH 7.5, 25°C, 40 mM (NH4)2SO4, 0.5 mg/ml bovine serum albumin
-
-
r
adenine + deoxyguanosine
deoxyadenosine + guanine
-
46.6% conversion after 4 h at standard reaction conditions: 2 mM each substrate, 0.013 U NTD, pH 7.5, 25°C, 40 mM (NH4)2SO4, 0.5 mg/ml bovine serum albumin
-
-
r
adenine + deoxyinosine
deoxyadenosine + hypoxanthine
-
57.3% conversion after 4 h at standard reaction conditions: 2 mM each substrate, 0.013 U NTD, pH 7.5, 25°C, 40 mM (NH4)2SO4, 0.5 mg/ml bovine serum albumin
-
-
r
adenine + deoxyuridine
deoxyadenosine + uracil
-
75.4% conversion after 4 h at standard reaction conditions: 2 mM each substrate, 0.013 U NTD, pH 7.5, 25°C, 40 mM (NH4)2SO4, 0.5 mg/ml bovine serum albumin
-
-
r
adenine + thymidine
deoxyadenosine + thymine
-
74.6% conversion after 4 h at standard reaction conditions: 2 mM each substrate, 0.013 U NTD, pH 7.5, 25°C, 40 mM (NH4)2SO4, 0.5 mg/ml bovine serum albumin
-
-
r
cytosine + deoxyadenosine
deoxycytidine + adenine
-
27.7% conversion after 4 h at standard reaction conditions: 2 mM each substrate, 0.013 U NTD, pH 7.5, 25°C, 40 mM (NH4)2SO4, 0.5 mg/ml bovine serum albumin
-
-
r
cytosine + deoxyguanosine
deoxycytidine + guanine
-
25.7% conversion after 4 h at standard reaction conditions: 2 mM each substrate, 0.013 U NTD, pH 7.5, 25°C, 40 mM (NH4)2SO4, 0.5 mg/ml bovine serum albumin
-
-
r
cytosine + deoxyinosine
deoxycytidine + hypoxanthine
-
32.0% conversion after 4 h at standard reaction conditions: 2 mM each substrate, 0.013 U NTD, pH 7.5, 25°C, 40 mM (NH4)2SO4, 0.5 mg/ml bovine serum albumin
-
-
r
cytosine + deoxyuridine
deoxycytidine + uracil
-
62.9% conversion after 4 h at standard reaction conditions: 2 mM each substrate, 0.013 U NTD, pH 7.5, 25°C, 40 mM (NH4)2SO4, 0.5 mg/ml bovine serum albumin
-
-
r
dAMP + 2,6-dichloropurine
adenine + 2,6-dichloropurin-9-yl-2'-deoxy-beta-D-ribose 5'-phosphate
-
-
-
-
?
dCMP + 2,6-dichloropurine
2,6-dichloropurin-9-yl-2'-deoxy-beta-D-ribose 5'-phosphate + cytosine
reaction is catalyzed by mutants F13R/D92S/N123S and F13Q/D92S/N123S
-
-
?
deoxyadenosine + 2-amino 6-methylmercaptopurine
adenine + 9-(2-deoxy-beta-D-ribofuranosyl)-2-amino-6-methylmercaptopurine
-
-
-
-
?
deoxyadenosine + 6-chloroguanine
adenine + 2'-deoxy-6-chloroguanosine
-
-
-
-
?
deoxyadenosine + 6-chloropurine
adenine + 9-(2-deoxy-beta-D-ribofuranosyl)-6-chloropurine
-
-
-
-
?
deoxyadenosine + 6-dimethylaminopurine
adenine + 9-(2-deoxy-beta-D-ribofuranosyl)-6-dimethylaminopurine
-
-
-
-
?
deoxyadenosine + 6-methylpurine
adenine + 9-(2-deoxy-beta-D-ribofuranosyl)-6-methylpurine
-
-
-
-
?
deoxyadenosine + ethyl 4-amino-1H-imidazole-5-carboxylate
adenine + ethyl 4-amino-1-(2-deoxy-b-D-erythro-pentofuranosyl)-1H-imidazole-5-carboxylate
-
-
-
-
?
deoxyadenosine + hypoxanthine
adenine + deoxyinosine + 7-(beta-D-2'-deoxyribufuranosyl)hypoxanthine
deoxycytidine + 1,7-dimethylguanine
cytosine + deoxy-1,7-dimethylguanosine
-
DRTase II
-
-
?
deoxycytidine + 1,N2-epsilon-guanine
cytosine + ?
-
deoxyribosylation at the N9 atom
-
-
?
deoxycytidine + 1,N6-epsilon-adenine
cytosine + ?
-
deoxyribosylation at the N9 atom
-
-
?
deoxycytidine + 3,N4-epsilon-cytosine
cytosine + ?
-
deoxyribosylation at the N9 atom
-
-
?
deoxycytidine + 5,6,7,9-tetrahydro-7-acetoxy-9-oxoimidazo[1,2-a]purine
cytosine + ?
-
deoxyribosylation at the N9 atom
-
-
?
deoxycytidine + C8-aminoguanine
cytosine + 2'-deoxy-8-aminoguanosine
-
deoxyribosylation at the N9 atom
-
-
?
deoxycytidine + C8-methylguanine
cytosine + 2'-deoxy-8-methylguanosine
-
deoxyribosylation at the N9 atom
-
-
?
deoxycytidine + hypoxanthine
cytosine + deoxyinosine
-
-
-
-
r
deoxycytidine + N2,3-epsilon-guanine
cytosine + ?
-
deoxyribosylation at the N9 atom
-
-
?
deoxycytidine + N2-(2-oxoethyl)guanine
cytosine + 2'-deoxy-N2-(2-oxoethyl)guanosine
-
deoxyribosylation at the N9 atom
-
-
?
deoxycytidine + pyrimidopurin-10-one
cytosine + ?
-
deoxyribosylation at the N9 atom
-
-
?
deoxyinosine + 1,7-dimethylguanine
hypoxanthine + deoxy-1,7-dimethylguanosine
-
DRTase I
-
-
?
deoxyinosine + 6-chloroguanine
hypoxanthine + deoxy-6-chloroguanosine
-
DRTase I
-
-
?
deoxyinosine + 8-azaguanine
hypoxanthine + deoxy-8-azaguanosine
-
-
-
-
r
deoxyinosine + 8-azaxanthine
hypoxanthine + deoxy-8-azaxanthosine
-
-
-
-
r
deoxyinosine + uric acid
hypoxanthine + 9-(2-deoxy-beta-D-ribofuranosyl)-7,9-dihydro-3H-purine-2,6,8-trione
-
-
-
-
r
hypoxanthine + deoxyadenosine
deoxyinosine + adenine
-
38.0% conversion after 4 h at standard reaction conditions: 2 mM substrate, 0.013 U NTD, pH 7.5, 25°C, 40 mM (NH4)2SO4, 0.5 mg/ml bovine serum albumin
-
-
r
hypoxanthine + deoxycytidine
deoxyinosine + cytosine
-
51.9% conversion after 4 h at standard reaction conditions: 2 mM each substrate, 0.013 U NTD, pH 7.5, 25°C, 40 mM (NH4)2SO4, 0.5 mg/ml bovine serum albumin
-
-
r
hypoxanthine + deoxyguanosine
deoxyinosine + guanine
-
25.2% conversion after 4 h at standard reaction conditions: 2 mM substrate, 0.013 U NTD, pH 7.5, 25°C, 40 mM (NH4)2SO4, 0.5 mg/ml bovine serum albumin
-
-
r
hypoxanthine + deoxyuridine
deoxyinosine + uracil
-
65.6% conversion after 4 h at standard reaction conditions: 2 mM substrate, 0.013 U NTD, pH 7.5, 25°C, 40 mM (NH4)2SO4, 0.5 mg/ml bovine serum albumin
-
-
r
hypoxanthine + thymidine
deoxyinosine + thymine
-
76.8% conversion after 4 h at standard reaction conditions: 2 mM substrate, 0.013 U NTD, pH 7.5, 25°C, 40 mM (NH4)2SO4, 0.5 mg/ml bovine serum albumin
-
-
r
purine base + 2-deoxy-D-ribosyl-base
purine deoxyribonucleoside + base
-
-
preferred over pyrimidine deoxyribonucleoside synthesis
-
r
pyrimidine base + 2-deoxy-D-ribosyl-base
pyrimidine deoxyribonucleoside + base
-
-
-
-
r
thymidine + 1-deaza-8-aza-adenine
thymine + 2'-deoxy-1-deaza-8-aza-adenosine
-
21% of substrate converted into deoxynucleoside after 2 h
-
-
?
thymidine + 1-deaza-adenine
thymine + 2'-deoxy-1-deaza-adenosine
-
6.2% of substrate converted into deoxynucleoside after 2 h
-
-
?
thymidine + 2,4-di-tert-butoxy-5-(1H-imidazol-4-yl)pyrimidine
2,4-di-tert-butoxy-5-(1-beta-D-ribofuranosyl-1H-imidazol-4-yl)pyrimidine + thymine
-
-
-
-
?
thymidine + 2,6-diaminopurine
thymine + 9-(2-deoxy-beta-D-ribofuranosyl)-2,6-diaminopurine
-
53.4% of substrate converted into deoxynucleoside after 2 h
-
-
?
thymidine + 2-(1H-imidazol-4-yl)benzaldehyde
2-[1-(2-deoxy-beta-D-ribofuranosyl)-1H-imidazol-4-yl]-benzaldehyde + thymine
-
-
-
-
?
thymidine + 2-amino-6-methyl-mercaptopurine
thymine + 9-(2-deoxy-beta-D-ribofuranosyl)-2-amino-6-methylmercaptopurine
-
-
-
-
r
thymidine + 2-aminopurine
thymine + 9-(2-deoxy-beta-D-ribofuranosyl)-2-aminopurine
-
-
-
-
r
thymidine + 2-fluoro-3-(1H-imidazol-4-yl)pyridine
2-fluoro-3-[1-(2-deoxy-beta-D-ribofuranosyl)-1H-imidazol-4-yl]-pyridine + thymine
-
-
-
-
?
thymidine + 3-(1H-imidazol-4-yl)pyridine
3-[1-(2-deoxy-beta-D-ribofuranosyl)-1H-imidazol-4-yl]-pyridine + thymine
-
-
-
-
?
thymidine + 3-bromo-5-(1H-imidazol-4-yl)pyridine
5-bromo-3-[1-(2-deoxy-beta -D-ribofuranosyl)-1H-imidazol-4-yl]-pyridin + thymine
-
-
-
-
?
thymidine + 3-deaza-8-aza-2-aminopurine
thymine + 9-(2-deoxy-beta-D-ribofuranosyl)-3-deaza-8-aza-aminopurine
-
39.7% of substrate converted into deoxynucleoside after 2 h
-
-
?
thymidine + 3-deaza-8-aza-adenine
thymine + 2'-deoxy-3-deaza-8-aza-adenosine
-
3.5% of substrate converted into deoxynucleoside after 2 h
-
-
?
thymidine + 4-(1-benzofuran-2-yl)-1H-imidazole
1-(2-deoxy-beta-D-ribofuranosyl)-4-(benzofuran-2-yl)-1H-imidazole + thymine
-
-
-
-
?
thymidine + 4-(1H-imidazol-4-yl)benzonitrile
4-[1-(2-deoxy-beta-D-ribofuranosyl)-1H-imidazol-4-yl]-benzonitrile + thymine
-
-
-
-
?
thymidine + 4-(2,4-difluorophenyl)-1H-imidazole
1-(2-deoxy-beta-D-ribofuranosyl)-4-(2,4-difluorophenyl)-1H-imidazole + thymine
-
-
-
-
?
thymidine + 4-(4-ethoxyphenyl)-1H-imidazole
1-(2-deoxy-beta-D-ribofuranosyl)-4-(4-ethoxyphenyl)-1H-imidazole + thymine
-
-
-
-
?
thymidine + 4-(4-methoxyphenyl)-1H-imidazole
1-(2-deoxy-beta-D-ribofuranosyl)-4-(4-methoxyphenyl)-1H-imidazole + thymine
-
-
-
-
?
thymidine + 4-(6-methoxynaphthalen-2-yl)-1H-imidazole
1-(2-deoxy-beta-D-ribofuranosyl)-4-(6-methoxynaphthalen-2-yl)-1H-imidazole + thymine
-
-
-
-
?
thymidine + 4-(dibenzo[b,d]furan-4-yl)-1H-imidazole
1-(2-deoxy-beta-D-ribofuranosyl)-4-(dibenzo[b,d]furan-4-yl)-1H-imidazole + thymine
-
-
-
-
?
thymidine + 4-(naphthalen-2-yl)-1H-imidazole
1-(2-deoxy-beta-D-ribofuranosyl)-4-(naphthalen-2-yl)-1H-imidazole + thymine
-
-
-
-
?
thymidine + 4-([1,1'-biphenyl]-3-yl)-1H-imidazole
1-(2-deoxy-beta-D-ribofuranosyl)-4-(biphenyl-3-yl)-1H-imidazole + thymine
-
-
-
-
?
thymidine + 4-([1,1'-biphenyl]-4-yl)-1H-imidazole
1-(2-deoxy-beta-D-ribofuranosyl)-4-(biphenyl-4-yl)-1H-imidazole + thymine
-
-
-
-
?
thymidine + 4-([11,21:23,31-terphenyl]-25-yl)-1H-imidazole
1-(2-deoxy-beta-D-ribofuranosyl)-4-[(3,5-diphenyl)phenyl]-1H-imidazole + thymine
-
-
-
-
?
thymidine + 4-amino-5-carboxamide-imidazole
thymine + ?
-
12.3% of substrate converted into deoxynucleoside after 2 h
-
-
?
thymidine + 4-amino-pyrazolopyrimidine
thymine + ?
-
0.37% of substrate converted into deoxynucleoside after 2 h
-
-
?
thymidine + 4-phenyl-1H-imidazole
1-(2-deoxy-beta-D-ribofuranosyl)-4-phenyl-1H-imidazole + thymine
-
-
-
-
?
thymidine + 5,6-dimethyl-benzimidazole
thymine + ?
-
37.7% of substrate converted into deoxynucleoside after 2 h
-
-
?
thymidine + 5-(1H-imidazol-4-yl)-1H-indazole
1-(2-deoxy-beta-D-ribofuranosyl)-4-(1H-indazol-5-yl)-1H-imidazole + thymine
-
-
-
-
?
thymidine + 5-(1H-imidazol-4-yl)-1H-indole
1-(2-deoxy-beta-D-ribofuranosyl)-4-(1H-indol-5-yl)-1H-imidazole + thymine
-
-
-
-
?
thymidine + 5-(1H-imidazol-4-yl)-2,4-dimethoxypyrimidine
2,4-dimethoxy-5-[1-(2-deoxy-beta-D-ribofuranosyl)-1H-imidazol-4-yl]-pyrimidine + thymine
-
-
-
-
?
thymidine + 5-(1H-imidazol-4-yl)pyrimidin-2-amine
2-amino-5-[1-(2-deoxy-beta-D-ribofuranosyl)-1H-imidazol-4-yl]-pyrimidine + thymine
-
-
-
-
?
thymidine + 5-(1H-imidazol-4-yl)pyrimidine
5-[1-(2-deoxy-beta-D-ribofuranosyl)-1H-imidazol-4-yl]-pyrimidine + thymine
-
-
-
-
?
thymidine + 5-bromouracil
thymine + 2'-deoxy-5-bromouridine
-
-
-
-
?
thymidine + 5-fluorouracil
thymine + 2'-deoxy-5-fluorouridine
-
-
-
-
?
thymidine + 5-hydroxyuracil
thymine + 2'-deoxy-5-hydroxyuridine
-
-
-
-
?
thymidine + 5-methylcytosine
thymine + 2'-deoxy-5-methylcytosine
-
-
-
-
?
thymidine + 5-nitrouracil
thymine + 2'-deoxy-5-nitrouridine
-
-
-
-
?
thymidine + 6-benzylaminopurine
thymine + 9-(2-deoxy-beta-D-ribofuranosyl)-6-benzylaminopurine
-
-
-
-
r
thymidine + 6-chloropurine
thymine + 9-(2-deoxy-beta-D-ribofuranosyl)-6-chloropurine
-
-
-
-
r
thymidine + 6-dimethylaminopurine
thymine + 9-(2-deoxy-beta-D-ribofuranosyl)-6-dimethylaminopurine
-
-
-
-
r
thymidine + 6-iodopurine
thymine + 9-(2-deoxy-beta-D-ribofuranosyl)-6-iodopurine
-
65.6% of substrate converted into deoxynucleoside after 2 h
-
-
?
thymidine + 6-mercaptoguanine
thymine + 6-mercaptoguanosine
-
52.1% of substrate converted into deoxynucleoside after 2 h
-
-
?
thymidine + 6-mercatopurine
thymine + 9-(2-deoxy-beta-D-ribofuranosyl)-6-mercaptopurine
-
56.5% of substrate converted into deoxynucleoside after 2 h
-
-
?
thymidine + 6-methoxypurine
thymine + 9-(2-deoxy-beta-D-ribofuranosyl)-6-methoxypurine
-
-
-
-
r
thymidine + 6-methylaminopurine
thymine + 9-(2-deoxy-beta-D-ribofuranosyl)-6-methylaminopurine
-
-
-
-
r
thymidine + 6-methylpurine
thymine + 9-(2-deoxy-beta-D-ribofuranosyl)-6-methylpurine
-
-
-
-
r
thymidine + 6-methylthio-8-trifluoromethylpurine
thymine + 9-(2-deoxy-beta-D-ribofuranosyl)-6-methylthio-8-trifluoromethylpurine
-
-
-
-
?
thymidine + 6-n-hexylaminopurine
thymine + 9-(2-deoxy-beta-D-ribofuranosyl)-6-n-hexylaminopurine
-
-
-
-
r
thymidine + 6-n-hexylmercaptopurine
thymine + 9-(2-deoxy-beta-D-ribofuranosyl)-6-n-hexylmercaptopurine
-
-
-
-
r
thymidine + 6-phenylaminopurine
thymine + 9-(2-deoxy-beta-D-ribofuranosyl)-6-phenylaminopurine
-
-
-
-
r
thymidine + 7-hydroxy-1,2,5-selenadiazolopyridine
thymine + ?
-
29.2% of substrate converted into deoxynucleoside after 2 h
-
-
?
thymidine + 8-aza-2,6-diaminopurine
thymine + 9-(2-deoxy-beta-D-ribofuranosyl)-8-aza-2,6-diaminopurine
-
59.6% of substrate converted into deoxynucleoside after 2 h
-
-
?
thymidine + 8-azaguanine
thymine + 2'-deoxy-8-azaguanosine
-
16.0% of substrate converted into deoxynucleoside after 2 h
-
-
?
thymidine + 8-azaxanthine
thymine + 2'-deoxy-8-azaxanthosine
-
-
-
-
?
thymidine + 8-bromoadenine
thymine + 2'-deoxy-8-bromoadenosine
-
-
-
?
thymidine + 8-chloroadenine
thymine + 2'-deoxy-8-chloroadenosine
-
-
3-deoxyribonucleoside + 9-deoxyribonucleoside
?
thymidine + 8-chlorotheophylline
thymine + 9-(2-deoxy-beta-D-ribofuranosyl)-8-chlorotheophylline
-
-
-
-
?
thymidine + 8-methyladenine
thymine + 2'-deoxy-8-methyladenosine
-
-
-
-
?
thymidine + 8-trifluoromethyladenine
thymine + 2'-deoxy-8-trifluoromethyladenosine
-
-
-
-
?
thymidine + benzimidazole
thymine + ?
-
33.1% of substrate converted into deoxynucleoside after 2 h
-
-
?
thymidine + methyl 3-(1H-imidazol-4-yl)benzoate
methyl 3-[1-(2-deoxy-beta-D-ribofuranosyl)-1H-imidazol-4-yl]-benzoate + thymine
-
-
-
-
?
thymidine + purine
thymine + 9-(2-deoxy-beta-D-ribofuranosyl)-purine
-
-
-
-
r
thymidine + theophylline
thymine + 2'-deoxytheophylline riboside
-
-
-
-
?
thymidine + xanthine
9-deoxyribosylxanthine + 7-deoxyribosylxanthine
-
-
20% 9-deoxyribosylxanthine + 80% 7-deoxyribosylxanthine
?
thymidine + [4-(1H-imidazol-4-yl)phenyl]methanol
1-(2-deoxy-beta-D-ribofuranosyl)-4-(4-hydroxymethylphenyl)-1H-imidazole + thymine
-
-
-
-
?
thymine + deoxyadenosine
thymidine + adenine
-
22.7% conversion after 4 h at standard reaction conditions: 2 mM each substrate, 0.013 U NTD, pH 7.5, 25°C, 40 mM (NH4)2SO4, 0.5 mg/ml bovine serum albumin
-
-
r
thymine + deoxycytidine
thymidine + cytosine
-
38.3% conversion after 4 h at standard reaction conditions: 2 mM each substrate, 0.013 U NTD, pH 7.5, 25°C, 40 mM (NH4)2SO4, 0.5 mg/ml bovine serum albumin
-
-
r
thymine + deoxyguanosine
thymidine + guanine
-
17.7% conversion after 4 h at standard reaction conditions: 2 mM each substrate, 0.013 U NTD, pH 7.5, 25°C, 40 mM (NH4)2SO4, 0.5 mg/ml bovine serum albumin
-
-
r
thymine + deoxyinosine
thymidine + hypoxanthine
-
19.5% conversion after 4 h at standard reaction conditions: 2 mM each substrate, 0.013 U NTD, pH 7.5, 25°C, 40 mM (NH4)2SO4, 0.5 mg/ml bovine serum albumin, equilibrium reached after 2 h
-
-
r
thymine + deoxyuridine
thymidine + uracil
-
53.7% conversion after 4 h at standard reaction conditions: 2 mM each substrate, 0.013 U NTD, pH 7.5, 25°C, 40 mM (NH4)2SO4, 0.5 mg/ml bovine serum albumin
-
-
r
uracil + deoxyadenosine
deoxyuridine + adenine
-
17.1% conversion after 4 h at standard reaction conditions: 2 mM each substrate, 0.013 U NTD, pH 7.5, 25°C, 40 mM (NH4)2SO4, 0.5 mg/ml bovine serum albumin
-
-
r
uracil + deoxycytidine
deoxyuridine + cytosine
-
37.7% conversion after 4 h at standard reaction conditions: 2 mM each substrate, 0.013 U NTD, pH 7.5, 25°C, 40 mM (NH4)2SO4, 0.5 mg/ml bovine serum albumin
-
-
r
uracil + deoxyguanosine
deoxyuridine + guanine
-
16.1% conversion after 4 h at standard reaction conditions: 2 mM each substrate, 0.013 U NTD, pH 7.5, 25°C, 40 mM (NH4)2SO4, 0.5 mg/ml bovine serum albumin
-
-
r
uracil + deoxyinosine
deoxyuridine + hypoxanthine
-
23.8% conversion after 4 h at standard reaction conditions: 2 mM each substrate, 0.013 U NTD, pH 7.5, 25°C, 40 mM (NH4)2SO4, 0.5 mg/ml bovine serum albumin
-
-
r
uracil + thymidine
deoxyuridine + thymine
-
43.0% conversion after 4 h at standard reaction conditions: 2 mM each substrate, 0.013 U NTD, pH 7.5, 25°C, 40 mM (NH4)2SO4, 0.5 mg/ml bovine serum albumin
-
-
r
2',3'-dideoxycytidine + adenine
2',3'-dideoxyadenosine + cytosine
-
-
-
?
2',3'-dideoxycytidine + adenine
2',3'-dideoxyadenosine + cytosine
-
-
-
-
?
2',3'-dideoxycytidine + adenine
2',3'-dideoxyadenosine + cytosine
-
-
-
?
2',3'-dideoxycytidine + adenine
2',3'-dideoxyadenosine + cytosine
-
-
-
?
2',3'-dideoxycytidine + hypoxanthine
2',3'-dideoxyinosine + cytosine
-
-
-
?
2',3'-dideoxycytidine + hypoxanthine
2',3'-dideoxyinosine + cytosine
-
-
-
-
?
2',3'-dideoxycytidine + hypoxanthine
2',3'-dideoxyinosine + cytosine
-
-
-
?
2',3'-dideoxycytidine + hypoxanthine
2',3'-dideoxyinosine + cytosine
-
-
-
?
2',3'-dideoxyguanosine + thymine
2',3'-dideoxythymidine + guanine
-
-
-
?
2',3'-dideoxyguanosine + thymine
2',3'-dideoxythymidine + guanine
-
-
-
?
2',3'-dideoxyguanosine + thymine
2',3'-dideoxythymidine + guanine
-
-
-
?
2',3'-dideoxyinosine + adenine
2',3'-dideoxyadenosine + hypoxanthine
-
-
-
?
2',3'-dideoxyinosine + adenine
2',3'-dideoxyadenosine + hypoxanthine
-
-
-
?
2',3'-dideoxyinosine + adenine
2',3'-dideoxyadenosine + hypoxanthine
-
-
-
?
2',3'-dideoxythymidine + adenine
2',3'-dideoxyadenosine + thymine
-
-
-
?
2',3'-dideoxythymidine + adenine
2',3'-dideoxyadenosine + thymine
-
-
-
-
?
2',3'-dideoxythymidine + adenine
2',3'-dideoxyadenosine + thymine
-
-
-
?
2',3'-dideoxythymidine + adenine
2',3'-dideoxyadenosine + thymine
-
-
-
?
2',3'-dideoxyuridine + cytosine
2',3'-dideoxycytidine + uracil
-
-
-
?
2',3'-dideoxyuridine + cytosine
2',3'-dideoxycytidine + uracil
-
-
-
?
2',3'didehydro-2',3'-dideoxythymidine + adenine
2',3'didehydro-2',3'-dideoxyadenosine + thymine
-
-
-
?
2',3'didehydro-2',3'-dideoxythymidine + adenine
2',3'didehydro-2',3'-dideoxyadenosine + thymine
-
-
-
-
?
2',3'didehydro-2',3'-dideoxythymidine + adenine
2',3'didehydro-2',3'-dideoxyadenosine + thymine
-
-
-
?
2'-deoxy-D-ribosyl-uracil + adenine
2'-deoxy-D-ribosyl-adenine + uracil
-
-
-
-
r
2'-deoxy-D-ribosyl-uracil + adenine
2'-deoxy-D-ribosyl-adenine + uracil
-
-
-
-
r
2'-deoxy-D-ribosyl-uracil + adenine
2'-deoxy-D-ribosyl-adenine + uracil
-
-
-
-
r
2'-deoxy-D-ribosyl-uracil + adenine
2'-deoxy-D-ribosyl-adenine + uracil
-
-
-
-
r
2'-deoxy-D-ribosyl-uracil + adenine
2'-deoxy-D-ribosyl-adenine + uracil
-
-
-
-
r
2'-deoxy-D-ribosyl-uracil + adenine
2'-deoxy-D-ribosyl-adenine + uracil
-
-
-
-
r
2'-deoxy-D-ribosyl-uracil + adenine
2'-deoxy-D-ribosyl-adenine + uracil
-
-
-
-
r
2'-deoxy-D-ribosyl-uracil + adenine
2'-deoxy-D-ribosyl-adenine + uracil
-
-
-
-
r
2'-deoxyadenosine + 5-chlorouracil
2'-deoxy-D-ribosyl-5-chlorouracil + adenine
-
-
-
-
r
2'-deoxyadenosine + 5-chlorouracil
2'-deoxy-D-ribosyl-5-chlorouracil + adenine
-
-
-
-
r
2'-deoxyadenosine + 5-chlorouracil
2'-deoxy-D-ribosyl-5-chlorouracil + adenine
-
-
-
-
r
2'-deoxyadenosine + 5-chlorouracil
2'-deoxy-D-ribosyl-5-chlorouracil + adenine
-
-
-
-
r
2'-deoxyadenosine + 5-chlorouracil
2'-deoxy-D-ribosyl-5-chlorouracil + adenine
-
-
-
?
2'-deoxyadenosine + 5-chlorouracil
2'-deoxy-D-ribosyl-5-chlorouracil + adenine
-
-
-
-
?
2'-deoxyadenosine + 5-chlorouracil
2'-deoxy-D-ribosyl-5-chlorouracil + adenine
-
-
-
-
r
2'-deoxyadenosine + 5-chlorouracil
2'-deoxy-D-ribosyl-5-chlorouracil + adenine
-
-
-
-
r
2'-deoxyadenosine + 5-chlorouracil
2'-deoxy-D-ribosyl-5-chlorouracil + adenine
-
-
-
-
r
2'-deoxyadenosine + 5-chlorouracil
2'-deoxy-D-ribosyl-5-chlorouracil + adenine
-
-
-
-
r
2'-deoxyadenosine + 5-fluorouracil
2'-deoxy-5-fluorouridine + adenine
-
-
-
-
r
2'-deoxyadenosine + 5-fluorouracil
2'-deoxy-5-fluorouridine + adenine
-
-
-
-
r
2'-deoxyadenosine + 5-fluorouracil
2'-deoxy-5-fluorouridine + adenine
-
-
-
-
r
2'-deoxyadenosine + 5-fluorouracil
2'-deoxy-5-fluorouridine + adenine
-
-
-
-
r
2'-deoxyadenosine + 5-fluorouracil
2'-deoxy-5-fluorouridine + adenine
-
-
-
-
r
2'-deoxyadenosine + 5-fluorouracil
2'-deoxy-5-fluorouridine + adenine
-
-
-
-
r
2'-deoxyadenosine + 5-fluorouracil
2'-deoxy-5-fluorouridine + adenine
-
-
-
-
r
2'-deoxyadenosine + 5-fluorouracil
2'-deoxy-5-fluorouridine + adenine
-
-
-
-
r
2'-deoxycytidine + adenine
2'-deoxyadenosine + cytosine
-
-
-
?
2'-deoxycytidine + adenine
2'-deoxyadenosine + cytosine
-
-
-
?
2'-deoxycytidine + adenine
2'-deoxyadenosine + cytosine
-
-
-
?
2'-deoxycytidine + hypoxanthine
2'-deoxyinosine + cytosine
-
-
-
?
2'-deoxycytidine + hypoxanthine
2'-deoxyinosine + cytosine
-
-
-
?
2'-deoxyguanosine + thymine
2'-deoxythymidine + guanine
-
-
-
?
2'-deoxyguanosine + thymine
2'-deoxythymidine + guanine
-
-
-
?
2'-deoxyinosine + adenine
2'-deoxyadenosine + hypoxanthine
-
-
-
?
2'-deoxyinosine + adenine
2'-deoxyadenosine + hypoxanthine
-
-
-
?
2'-deoxythymidine + adenine
2'-deoxyadenosine + thymine
-
-
-
?
2'-deoxythymidine + adenine
2'-deoxyadenosine + thymine
-
-
-
?
2'-deoxyuridine + 2,6-diaminopurine
2,6-diaminopurine 2-deoxy-D-ribose 5-phosphate + uracil
-
-
-
?
2'-deoxyuridine + 2,6-diaminopurine
2,6-diaminopurine 2-deoxy-D-ribose 5-phosphate + uracil
-
-
-
-
?
2'-deoxyuridine + 5-fluorocytosine
2'-deoxy-5-fluorocytidine + uracil
-
-
-
?
2'-deoxyuridine + 5-fluorocytosine
2'-deoxy-5-fluorocytidine + uracil
-
-
-
-
?
2'-deoxyuridine + 5-trifluorothymine
5-trifluorothymidine + uracil
-
-
-
-
?
2'-deoxyuridine + 5-trifluorothymine
5-trifluorothymidine + uracil
-
-
-
-
?
2'-deoxyuridine + 6-mercaptopurine
6-mercaptopurine 2-deoxy-D-ribose 5-phosphate + uracil
-
-
-
?
2'-deoxyuridine + 6-mercaptopurine
6-mercaptopurine 2-deoxy-D-ribose 5-phosphate + uracil
-
-
-
-
?
2'-deoxyuridine + adenine
2'-deoxyadenosine + uracil
-
-
-
-
?
2'-deoxyuridine + adenine
2'-deoxyadenosine + uracil
-
-
-
-
?
2'-deoxyuridine + benzimidazole
benzimidazole 2-deoxy-D-ribose 5-phosphate + uracil
-
-
-
?
2'-deoxyuridine + benzimidazole
benzimidazole 2-deoxy-D-ribose 5-phosphate + uracil
-
-
-
-
?
2'-deoxyuridine + cytosine
2'-deoxycytidine + uracil
-
-
-
?
2'-deoxyuridine + thymidine
2'-deoxythymidine + uracil
-
-
-
-
?
2'-deoxyuridine + thymidine
2'-deoxythymidine + uracil
-
-
-
-
?
2-deoxy-D-ribosyl-base1 + base2
2-deoxy-D-ribosyl-base2 + base1
-
-
-
-
?
2-deoxy-D-ribosyl-base1 + base2
2-deoxy-D-ribosyl-base2 + base1
-
-
-
-
?
2-deoxy-D-ribosyl-base1 + base2
2-deoxy-D-ribosyl-base2 + base1
-
-
-
-
?
cytosine + thymidine
deoxycytidine + thymine
-
53.5% conversion after 4 h at standard reaction conditions: 2 mM each substrate, 0.013 U NTD, pH 7.5, 25°C, 40 mM (NH4)2SO4, 0.5 mg/ml bovine serum albumin
-
-
r
cytosine + thymidine
deoxycytidine + thymine
-
standard reaction for cofactor analyses as well as activating compound and inhibitor studies, in presence of 40 mM ammonium sulfate and 0.5 mg/ml bovine serum albumin
-
-
r
deoxyadenosine + cytosine
adenine + deoxycytidine
-
DRTase II
-
-
?
deoxyadenosine + hypoxanthine
adenine + deoxyinosine + 7-(beta-D-2'-deoxyribufuranosyl)hypoxanthine
-
-
-
?
deoxyadenosine + hypoxanthine
adenine + deoxyinosine + 7-(beta-D-2'-deoxyribufuranosyl)hypoxanthine
-
-
-
-
r
deoxycytidine + adenine
cytosine + deoxyadenosine
-
-
-
-
r
deoxyinosine + adenine
hypoxanthine + deoxyadenosine
-
-
-
-
r
deoxyinosine + adenine
hypoxanthine + deoxyadenosine
-
DRTase I
-
-
r
deoxyinosine + cytosine
hypoxanthine + deoxycytidine
-
DRTase II
-
-
?
deoxyinosine + guanine
hypoxanthine + deoxyguanosine
-
DRTase I
-
-
-
thymidine + adenine
2'-deoxy-D-ribosyl-adenine + thymine
-
-
-
-
r
thymidine + adenine
2'-deoxy-D-ribosyl-adenine + thymine
-
-
-
-
r
thymidine + adenine
2'-deoxy-D-ribosyl-adenine + thymine
-
-
-
-
r
thymidine + adenine
2'-deoxy-D-ribosyl-adenine + thymine
-
-
-
-
r
thymidine + adenine
2'-deoxy-D-ribosyl-adenine + thymine
-
-
-
-
r
thymidine + adenine
2'-deoxyadenosine + thymine
-
-
-
-
?
thymidine + adenine
thymine + adenosine
-
-
3-deoxyribonucleoside
?
thymidine + cytosine
thymine + 2'-deoxycytidine
-
-
-
-
?
thymidine + guanine
thymine + 2'-deoxyguanosine
-
42.3% of substrate converted into deoxynucleoside after 2 h
-
-
?
additional information
?
-
-
the enzyme is selective for 6-aminopurine compared to 6-oxopurines and for 2'-deoxyuridine compared to thymidine
-
-
-
additional information
?
-
-
the enzyme is selective for 6-aminopurine compared to 6-oxopurines and for 2'-deoxyuridine compared to thymidine
-
-
-
additional information
?
-
-
the enzyme is selective for 6-aminopurine compared to 6-oxopurines and for 2'-deoxyuridine compared to thymidine
-
-
-
additional information
?
-
-
the enzyme is not able to interchange the deoxyribosyl moiety between ribonucleosides and is free of nucleoside phosphorylases traces
-
-
-
additional information
?
-
-
the enzyme is not able to interchange the deoxyribosyl moiety between ribonucleosides and is free of nucleoside phosphorylases traces
-
-
-
additional information
?
-
-
the enzyme is selective for 6-aminopurine compared to 6-oxopurines and for 2'-deoxyuridine compared to thymidine
-
-
-
additional information
?
-
-
the DEAE-Sepharose-immobilized enzyme is used to obtain 5-fluorouracil-2'-deoxyriboside (floxuridine), 6-chloropurine-2'-deoxyriboside, 6-bromopurine-2'-deoxyriboside, 6-chloro-2-fluoropurine-2'-deoxyriboside, 5-bromo-2'-deoxyuridine, and 5-chloro-2'-deoxyuridine
-
-
-
additional information
?
-
-
the enzyme glycosylates N2,3-ethenoguanine (8,9-dihydro-9-oxoimidazo[2,1-b]purine) at N7 and N1 with somewhat less selectivity for N7
-
-
-
additional information
?
-
-
nucleoside deoxyribosyltransferase I is strictly specific for transfer between purine bases. Nucleoside deoxyribosyltransferase II catalyzes the transfer of the deoxyribosyl moiety between purines or pyrimidines as well as from a purine to a pyrimidine
-
-
-
additional information
?
-
DRTase I is strictly specific for transfer between purine bases. DRTase II catalyzes the transfer of the deoxyribosyl moiety between purines or pyrimidines as well as from a purine to a pyrimidine
-
-
-
additional information
?
-
DRTase I is specific for purines with preference for deoxyinosine, deoxyadenosine, deoxyguanosine as donor substrates
-
-
-
additional information
?
-
specific role for DRTase I in the metabolism of deoxyinosine
-
-
-
additional information
?
-
-
DRTase I is strictly specific for transfer between purine bases. DRTase II catalyzes the transfer of the deoxyribosyl moiety between purines or pyrimidines as well as from a purine to a pyrimidine
-
-
-
additional information
?
-
-
no activity with 3'-azido-3'-deoxythymidine or 3'-amino-3'-deoxythymidine with adenine as acceptor
-
-
-
additional information
?
-
-
the enzyme accepts purines or pyrimidines as donor substrates, showing preference for 2'-deoxycytidine > 2'-deoxyuridine > 2'-deoxyadenosine and thymidine > 2'-deoxyguanosine >>> 2'-deoxyinosine, and a similar preference for pyrimidines and purines
-
-
-
additional information
?
-
enzyme does not interchange bases between ribonucleosides, but is able to catalyze the transfer of 2'-deoxyribose between purine or pyrimidine and/or pyrimidine bases. The enzyme prefers pyrimidines to purines as substrates, dUrd being the best nucleoside donor, followed by dThd, dAdo, and dIno, whereas cytosine is the best base acceptor and hypoxanthine is the worst
-
-
-
additional information
?
-
-
enzyme does not interchange bases between ribonucleosides, but is able to catalyze the transfer of 2'-deoxyribose between purine or pyrimidine and/or pyrimidine bases. The enzyme prefers pyrimidines to purines as substrates, dUrd being the best nucleoside donor, followed by dThd, dAdo, and dIno, whereas cytosine is the best base acceptor and hypoxanthine is the worst
-
-
-
additional information
?
-
-
the enzyme participates in the nucleoside salvage pathway
-
-
-
additional information
?
-
-
the enzyme is selective for 6-aminopurine compared to 6-oxopurines and for 2'-deoxyuridine compared to thymidine
-
-
-
additional information
?
-
-
the enzyme is selective for 6-aminopurine compared to 6-oxopurines and for 2'-deoxyuridine compared to thymidine
-
-
-
additional information
?
-
-
preferably purine 2-deoxyribonucleoside synthesis compared to pyrimidine deoxyribonucleoside synthesis although both are catalysed
-
-
-
additional information
?
-
-
ribonucleoside, arabinosylnucleoside and 2,3-dideoxyribonucleoside are no substrates for base-exchange by YejD
-
-
-
additional information
?
-
-
no activity with 3'-azido-3'-deoxythymidine or 3'-amino-3'-deoxythymidine with adenine as acceptor
-
-
-
additional information
?
-
-
the enzyme accepts purines or pyrimidines as donor substrates, showing preference for 2'-deoxycytidine > 2'-deoxyuridine > 2'-deoxyadenosine and thymidine > 2'-deoxyguanosine >>> 2'-deoxyinosine, and a similar preference for pyrimidines and purines
-
-
-
additional information
?
-
-
the enzyme is selective for 6-aminopurine compared to 6-oxopurines and for 2'-deoxyuridine compared to thymidine
-
-
-
additional information
?
-
-
the enzyme is selective for 6-aminopurine compared to 6-oxopurines and for 2'-deoxyuridine compared to thymidine
-
-
-
additional information
?
-
-
RCL cleaves the N-glycosidic bond of deoxynucleoside 5'-monophosphates to yield two reaction products, deoxyribose 5-phosphate and purine or pyrimidine base
-
-
-
additional information
?
-
-
the enzyme is important in the salvage pathway of nucleoside recycling, Trypanosoma brucei lacks enzymes of de novo purine biosynthesis
-
-
-
thymidine + 2-hydroxy-6-mercaptopurine
additional information
-
-
-
2 products
r
thymidine + 2-mercaptoadenine
additional information
-
-
-
2 products
r
thymidine + 2-mercaptopurine
additional information
-
-
-
2 products
r
thymidine + 6-hydrazinepurine
additional information
-
-
-
several products observed
r
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NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate)
(Substrate)
LITERATURE
(Substrate)
(Substrate)
COMMENTARY
(Product)
(Product)
LITERATURE
(Product)
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
r=reversible
ir=irreversible
?=not specified
purine base + 2-deoxy-D-ribosyl-base
purine deoxyribonucleoside + base
-
-
preferred over pyrimidine deoxyribonucleoside synthesis
-
r
pyrimidine base + 2-deoxy-D-ribosyl-base
pyrimidine deoxyribonucleoside + base
-
-
-
-
r
2'-deoxy-D-ribosyl-uracil + adenine
2'-deoxy-D-ribosyl-adenine + uracil
-
-
-
-
r
2'-deoxy-D-ribosyl-uracil + adenine
2'-deoxy-D-ribosyl-adenine + uracil
-
-
-
-
r
2'-deoxy-D-ribosyl-uracil + adenine
2'-deoxy-D-ribosyl-adenine + uracil
-
-
-
-
r
2'-deoxy-D-ribosyl-uracil + adenine
2'-deoxy-D-ribosyl-adenine + uracil
-
-
-
-
r
2'-deoxy-D-ribosyl-uracil + adenine
2'-deoxy-D-ribosyl-adenine + uracil
-
-
-
-
r
2'-deoxy-D-ribosyl-uracil + adenine
2'-deoxy-D-ribosyl-adenine + uracil
-
-
-
-
r
2'-deoxy-D-ribosyl-uracil + adenine
2'-deoxy-D-ribosyl-adenine + uracil
-
-
-
-
r
2'-deoxy-D-ribosyl-uracil + adenine
2'-deoxy-D-ribosyl-adenine + uracil
-
-
-
-
r
2-deoxy-D-ribosyl-base1 + base2
2-deoxy-D-ribosyl-base2 + base1
-
-
-
-
?
2-deoxy-D-ribosyl-base1 + base2
2-deoxy-D-ribosyl-base2 + base1
-
-
-
-
?
2-deoxy-D-ribosyl-base1 + base2
2-deoxy-D-ribosyl-base2 + base1
-
-
-
-
?
additional information
?
-
Q8RLY5
specific role for DRTase I in the metabolism of deoxyinosine
-
-
-
additional information
?
-
-
the enzyme participates in the nucleoside salvage pathway
-
-
-
additional information
?
-
-
preferably purine 2-deoxyribonucleoside synthesis compared to pyrimidine deoxyribonucleoside synthesis although both are catalysed
-
-
-
additional information
?
-
-
the enzyme is important in the salvage pathway of nucleoside recycling, Trypanosoma brucei lacks enzymes of de novo purine biosynthesis
-
-
-
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METALS and IONS
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
K+
-
enhances base exchange activity and thermostability, 1.66-fold activation by 1 mM KCl (reaches plateau at 20 mM)
NH4+
-
enhances base exchange activity and thermostability, 1.43-fold activation by 1 mM (NH4)2SO4 (reaches plateau at 20 mM)
additional information
additional information
-
enzymatic activity depends on monovalent cations as cofactors rather than catalysts; K+ and NH4+ have no synergistic effects; Li+ and Na+ do not activate
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INHIBITORS
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
amino-pyrrolo-pyrimidine
-
12.1 mM, complete inhibition of the reaction with adenine and deoxycytidine
Hydroxypyrrolopyrimidine
-
0.33 mM, 66% inhibition of the reaction with adenine and deoxycytidine
-
imidazole
-
12.1 mM, 76% inhibition of the reaction with adenine and deoxycytidine
deoxyadenosine
-
linear competitive inhibition of deoxyinosine and linear non-competitive inhibition of adenine
Tris
-
complete inhibition of deoxyribosyltransferase I at 0.1 M, pH 6.9. 62% inhibition of nucleoside deoxyribosyltransferase I at 0.8 M, pH 5.9
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ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
K+
-
K+-activated type II enzyme: binding of K+ is required to enhance activity through induced conformational change
additional information
-
does not require phosphate derivatives such as ATP, thiamine diphosphate, pyridoxal phosphate to complete 2-deoxyribose transfer
-
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KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
additional information
additional information
-
kinetics and substrate specifiicty
-
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TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
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kcat/KM VALUE [1/mMs-1]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
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Ki VALUE [mM]
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
0.01
6-methylthio-GMP
-
pH 7.5, 37°C, recombinant wild-type enzyme, versus dGMP
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SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
0.01
0.017
wild-type, with 2',3'-dideoxythymidine and adenine as substrates
0.03
0.036
0.04
mutant G9S, with 2',3'-dideoxyinosine and adenine as substrates; wild-type, with 2',3'-dideoxycytidine and hypoxanthine as substrates
0.05
0.056
wild-type, with 2',3'-dideoxyuridine and cytosine as substrates
0.06
0.19
-
wild-type, with 2',3'-dideoxyuridine and cytosine as substrates
0.2
mutant G9S, with 2',3'didehydro-2',3'-dideoxythymidine and adenine as substrates
0.25
mutant G9S, with 3'-dideoxythymidine and adenine as substrates
0.4
mutant G9S, with 2',3'-dideoxyguanosine and thymine as substrates
0.47
mutant G9S, with 2'-deoxyinosine and adenine as substrates
0.5
0.6
-
mutant G9S, with 2',3'-dideoxyguanosine and thymine as substrates
0.8
1.04
mutant G9S, with 2'-deoxythymidine and adenine as substrates
1.1
1.25
mutant G9S, with 2'-deoxycytidine and hypoxanthine as substrates
1.29
1.3
mutant G9S, with 2',3'-dideoxyuridine and cytosine as substrates
1.35
-
mutant A15T, 2',3'-dideoxyadenosine and cytosine as substrates
1.6
-
mutant G9S, with 2',3'-dideoxyuridine and cytosine as substrates
1.9
3.1
mutant G9S, with 2'-deoxyuridine and cytosine as substrates
3.6
wild-type, with 2'-deoxyinosine and adenine as substrates
6
mutant G9S, with 2',3'-dideoxycytidine and adenine as substrates
6.67
-
equals 100%, purified protein, in presence of 0.5 mg/ml bovine serum albumin, pH 7.5, 10 min, 25°C
6.8
6.9
7.1
9.28
9.3
11.9
wild-type, with 2'-deoxythymidine and adenine as substrates
20.1
27.1
mutant G9S, with 2'-deoxycytidine and adenine as substrates
29
wild-type, with 2',3'-dideoxycytidine and adenine as substrates
42.5
wild-type, with 2'-deoxyuridine and cytosine as substrates
83.7
173
wild-type, with 2'-deoxycytidine and adenine as substrates
additional information
0.01
-
wild-type, with 2',3'-dideoxyguanosine and thymine as substrates; wild-type, with 2',3'-dideoxyinosine and adenine as substrates
0.01
-
wild-type, 2',3'-dideoxyadenosine and thymine as substrates; wild-type, 2',3'-dideoxycytidine and thymine as substrates
0.01
wild-type, with 2',3'didehydro-2',3'-dideoxythymidine and adenine as substrates; wild-type, with 2',3'-dideoxyguanosine and thymine as substrates; wild-type, with 2',3'-dideoxyinosine and adenine as substrates; wild-type, with 2'-deoxycytidine and hypoxanthine as substrates
0.03
-
wild-type, with 2',3'-dideoxythymidine and adenine as substrates
0.03
-
wild-type, 2',3'-dideoxyadenosine and cytosine as substrates; wild-type, 2,3'-dideoxythymidine and adenine as substrates
0.036
-
wild-type, with 2',3'didehydro-2',3'-dideoxythymidine and adenine as substrates
0.036
-
wild-type, 2',3'didehydro-2',3'-dideoxythymidine and adenine as substrates
0.05
-
wild-type, with 2',3'-dideoxycytidine and hypoxanthine as substrates
0.05
-
wild-type, 2',3'-dideoxycytidine and hypoxanthine as substrates
0.06
-
mutant G9S, with 2',3'-dideoxyinosine and adenine as substrates; wild-type, with 2'-deoxycytidine and hypoxanthine as substrates
0.8
mutant G9S, with 2',3'-dideoxycytidine and hypoxanthine as substrates
1.29
-
mutant G9S, with 2',3'-dideoxycytidine and adenine as substrates
6.8
-
mutant G9S, with 2',3'-dideoxycytidine and hypoxanthine as substrates
6.8
-
mutant A15T, 2',3'-dideoxycytidine and hypoxanthine as substrates
6.9
-
wild-type, with 2',3'-dideoxycytidine and adenine as substrates
7.1
-
mutant G9S, with 2'-deoxycytidine and hypoxanthine as substrates
9.28
-
mutant G9S, with 2',3'didehydro-2',3'-dideoxythymidine and adenine as substrates
9.28
-
mutant A15T, 2',3'didehydro-2',3'-dideoxythymidine and adenine as substrates
additional information
-
method for determining enzyme activity by coupling the release of adenine from deoxyadenosine to the reaction catalyzed by pyruvate kinase/lactate dehydrogenase via adenine phosphoribosyltransferase and adenylate kinase
additional information
-
0.9% in presence of 1 mM ZnCl2; 102% in presence of 1 mM NaCl; 105% in presence of 1 mM CsCl; 106% in presence of 1 mM NaSO4; 12.9% in presence of 1 mM CoCl2; 143% in presence of 1 mM NH4Cl; 151% in presence of 1 mM RbCl; 163% in presence of 1 mM (NH4)2SO4; 165% in presence of 1 mM K2SO4; 166% in presence of 1 mM KCl; 166% in presence of 1 mM (NH4)2SO4 and 1 mM KCl; 192% in presence of 10 mM KCl; 194% in presence of 10 mM (NH4)2SO4; 3.2% in presence of 1 mM CuSO4; 51.8% in presence of 1 mM CaCl2; 66.3% in presence of 1 mM MgCl2; 66% in presence of 1 mM MgSO4; 68.3% in presence of 1 mM FeCl2; 97.7% in presence of 1 mM LiCl
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pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
6.5
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pH RANGE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
5 - 8
-
50% of maximal activity at pH 7.0 with substrate dCMP, 20% with dGMP, 10% of maximal activity at pH 8.0 with both substrates
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TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
40
57
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pI VALUE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
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SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
SOURCE
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LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY
GeneOntology No.
LITERATURE
SOURCE
-
attached to cell wall surface, enzyme can be released into the culture broth
-
attached to cell wall surface, enzyme can be released into the culture broth
-
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PDB
SCOP
CATH
ORGANISM
UNIPROT
Trypanosoma brucei brucei (strain 927/4 GUTat10.1)
Trypanosoma brucei brucei (strain 927/4 GUTat10.1)
Trypanosoma brucei brucei (strain 927/4 GUTat10.1)
Trypanosoma brucei brucei (strain 927/4 GUTat10.1)
Trypanosoma brucei brucei (strain 927/4 GUTat10.1)
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MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
17250
-
4 * 17250, elution as single peak from the preparative gel filtration column
17649
-
1 * 17649, unmodified recombinant enzyme, mass spectrometry, 1 * 19812, His-tagged recombinant enzyme, mass spectrometry, 1 * 20440, His-tagged recombinant enzyme, SDS-PAGE; 2 * 17649, unmodified recombinant enzyme, mass spectrometry, 2 * 17649, unmodified recombinant enzyme, mass spectrometry, 2 * 20440, His-tagged recombinant enzyme, SDS-PAGE
18000
19812
-
1 * 17649, unmodified recombinant enzyme, mass spectrometry, 1 * 19812, His-tagged recombinant enzyme, mass spectrometry, 1 * 20440, His-tagged recombinant enzyme, SDS-PAGE
25500
-
according to migration in SDS-12.5% polyacrylamide gel electrophoresis
110000
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SUBUNITS
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
dimer
-
2 * 17649, unmodified recombinant enzyme, mass spectrometry, 2 * 17649, unmodified recombinant enzyme, mass spectrometry, 2 * 20440, His-tagged recombinant enzyme, SDS-PAGE
hexamer
homohexamer
homotetramer
-
4 * 17250, elution as single peak from the preparative gel filtration column
monomer
-
1 * 17649, unmodified recombinant enzyme, mass spectrometry, 1 * 19812, His-tagged recombinant enzyme, mass spectrometry, 1 * 20440, His-tagged recombinant enzyme, SDS-PAGE
additional information
additional information
-
at 0.003 mM the His-RCL is mostly monomeric, at 0.03 mM, His-RCL is mostly dimeric, at 0.01 mM His-RCL is at equilibrium between monomeric and dimeric states, overview
additional information
-
ligand binding and active site structures, overview
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Crystallization/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
microdiffusion by means of equilibrium dialysis is used to crystallize the enzyme
-
purified recombinant selenomethionine-labeled enzyme free or in complex with benzo[cd]indol-2(1H)-one, 5-aminoisoquinoline, 2-ethylbenzyl alcohol, or 1-methylquinoline-2(1H)-one, fragment cocktail crystallography, X-ray diffraction structure determination and analysis at 1.5-1.8 A resolution, SAD X-ray techniques
-
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pH STABILITY
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
6 - 10
-
the enzyme is stable in a pH range from 6.0 to 9.0, and retains more than 85% activity at pH 10.0
736846
6.5
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TEMPERATURE STABILITY
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
25
-
pH 7.5, 20 min, 60% activity lost, in presence of 10 mM (NH4)2SO4 only 20% activity lost, in presence of 500 mM KCL only 6% activity lost
40
-
the magnetic chitosan bead-immobilized enzyme suffers no loss of enzymatic activity when stored at 40°C for at least 144 h
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GENERAL STABILITY
ORGANISM
UNIPROT
LITERATURE
the enzyme is stable when immobilized on PEI-agarose but shows no activity when immobilized on glyoxyl-agarose. The best stability is obtained using dextrane oxidized at 70% (w/v)
-
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ORGANIC SOLVENT
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
2-propanol
-
enzyme immobilized on Sepabeads shows 1.2-1.4fold enhanced activity at 20% of methanol, ethanol, 2-propanol, ethylene glycol, and acetone, and at 10% and 30% acetonitrile. Highest increase in activity is obtained in presence of 20% acetonitrile
Acetone
-
enzyme immobilized on Sepabeads shows 1.2-1.4fold enhanced activity at 20% of methanol, ethanol, 2-propanol, ethylene glycol, and acetone, and at 10% and 30% acetonitrile. Highest increase in activity is obtained in presence of 20% acetonitrile
acetonitrile
-
enzyme immobilized on Sepabeads shows 1.2-1.4fold enhanced activity at 20% of methanol, ethanol, 2-propanol, ethylene glycol, and acetone, and at 10% and 30% acetonitrile. Highest increase in activity is obtained in presence of 20% acetonitrile
Ethanol
-
enzyme immobilized on Sepabeads shows 1.2-1.4fold enhanced activity at 20% of methanol, ethanol, 2-propanol, ethylene glycol, and acetone, and at 10% and 30% acetonitrile. Highest increase in activity is obtained in presence of 20% acetonitrile
Ethylene glycol
-
enzyme immobilized on Sepabeads shows 1.2-1.4fold enhanced activity at 20% of methanol, ethanol, 2-propanol, ethylene glycol, and acetone, and at 10% and 30% acetonitrile. Highest increase in activity is obtained in presence of 20% acetonitrile
Methanol
-
enzyme immobilized on Sepabeads shows 1.2-1.4fold enhanced activity at 20% of methanol, ethanol, 2-propanol, ethylene glycol, and acetone, and at 10% and 30% acetonitrile. Highest increase in activity is obtained in presence of 20% acetonitrile
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STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
-20°C, purified recombinant enzyme, 1 mg/ml, at least 1 year without loss of activity
-
-50°C, stable for several months either in the lyophilized form or disolved in freshly distilled water
-
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Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
Bio-Scale Mini UNOsphere Q column chromatography, and Superose 12 gel filtration
-
preclearing of bacterial lysate with 40% ammonium sulfate and subsequent precipitation of proteins with 90% ammonium sulfate, gel filtration of dissolved protein on Sephacryl S-200 column, combination of fractions with N-deoxyribosyltransferase activity, precipitation of proteins with 90% ammonium sulfate, dialysis of dissolved proteins and concentration by ultrafiltration, second gel filtration on Sephacryl S-200 column, combination of active fractions and concentration by ultrafiltration, recovery of 70.4% of activity, storage at -20°C in 50% glycerol
-
recombinant His-tagged wild-type and selenomethionine-labeled enzymes from Escherichia coli to homogeneity by nickel affinity chromatography and gel filtration
-
recombinant unmodified enzyme from Escherichia coli strain Bli5 (BL21 (DE3) pDIA17) by anion exchange chromatography and gel filtration, recombinant His-tagged enzyme by nickel affinity chromatography, over 95% purity
-
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Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
expression in Escherichia coli
expression of His-tagged wild-type and selenomethionine-labeled enzymes in Escherichia coli
-
expression of the catalytically inactive mutant E98A in Escherichia coli
-
gene Rc1, expression of soluble unmodified enzyme and His-tagged enzymes in Escherichia coli strain Bli5 (BL21 (DE3) pDIA17)
-
in pET-22b(+) for expression in Escherichia coli BL21(DE3); in pGEM-T for cloning
-
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ENGINEERING
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
A15C
A15S
A15T
A15V
-
can not improve 2',3'-dideoxyribose transfer reaction, kcat is diminished as compared to wild-type
A15S
-
activity with 2'-deoxycytidine and adenine as substrates is closer to that of the wild-type enzyme than that of the A15T mutant, kcat is diminished as compared to wild-type
-
A15T
-
mutation allows conversion of the nucleoside 2'deoxyribosyltransferase activity to a nucleoside 2',3'-dideoxy- or 2',3'-didehydro,2',3'-dideoxyribosyltransferase activity, kcat is diminished as compared to wild-type
-
A15V
-
can not improve 2',3'-dideoxyribose transfer reaction, kcat is diminished as compared to wild-type
-
G9C
can not improve 2',3'-dideoxyribose transfer reaction, kcat is diminished as compared to wild-type
G9S
G9T
Ser remains a better substituent for Gly-9 than Thr, kcat is diminished as compared to wild-type
G9W
can not improve 2',3'-dideoxyribose transfer reaction, kcat is diminished as compared to wild-type
E105A
-
site-directed mutagenesis, the mutant shows about 200fold reduced activity and a 170fold increased Km compared to the wild-type enzyme
additional information
A15C
-
is not capable of improving 2',3'-dideoxyribose transfer reaction
A15S
-
activity with 2'-deoxycytidine and adenine as substrates is closer to that of the wild-type enzyme than that of the A15T mutant, kcat is diminished as compared to wild-type
A15S
-
Ser appears a good alternative for Thr, rate of 2',3'-dideoxyribosyl transfer from 2',3'-dideoxycytidine to adenine of the two mutants is almost identical
A15T
-
mutation allows conversion of the nucleoside 2'deoxyribosyltransferase activity to a nucleoside 2',3'-dideoxy- or 2',3'-didehydro,2',3'-dideoxyribosyltransferase activity, kcat is diminished as compared to wild-type
A15T
-
a single amino-acid substitution allows the conversion of the nucleoside 2'-deoxyribosyltransferase activity to a nucleoside 2',3'-dideoxy- or 2'3'-didehydro, 2',3'-dideoxyribosyltransferase activity. Specificity for the base is unchanged compared to the wild-type. The mutant displays an improved activity to the transfer of 2',3'-dideoxyribose, which is enhanced by 250fold (depending on the donors and acceptors) while the deoxyribosyl group transfer remains acceptable (30% of the wild-type)
G9S
mutation allows conversion of the nucleoside 2'deoxyribosyltransferase activity to a nucleoside 2',3'-dideoxy- or 2',3'-didehydro,2',3'-dideoxyribosyltransferase activity, kcat is diminished as compared to wild-type
G9S
-
a single amino-acid substitution allows the conversion of the nucleoside 2'-deoxyribosyltransferase activity to a nucleoside 2',3'-dideoxy- or 2'3'-didehydro, 2',3'-dideoxyribosyltransferase activity
additional information
-
immobilization on calcium alginate or calcium pectate of the enzyme increases the 2'-deoxynucleoside synthesis effiency of the organism, method optimization, overview
additional information
-
immobilization on calcium alginate or calcium pectate of the enzyme increases the 2'-deoxynucleoside synthesis effiency of the organism, method optimization, overview
-
additional information
-
immobilization on calcium alginate or calcium pectate of the enzyme increases the 2'-deoxynucleoside synthesis effiency of the organism, method optimization, overview
additional information
-
immobilization on calcium alginate or calcium pectate of the enzyme increases the 2'-deoxynucleoside synthesis effiency of the organism, method optimization, overview
-
additional information
-
immobilization on calcium alginate or calcium pectate of the enzyme increases the 2'-deoxynucleoside synthesis effiency of the organism, method optimization, overview
additional information
-
immobilization on calcium alginate or calcium pectate of the enzyme increases the 2'-deoxynucleoside synthesis effiency of the organism, method optimization, overview
-
additional information
-
immobilization on calcium alginate or calcium pectate of the enzyme increases the 2'-deoxynucleoside synthesis effiency of the organism, method optimization, overview
additional information
-
immobilization on calcium alginate or calcium pectate of the enzyme increases the 2'-deoxynucleoside synthesis effiency of the organism, method optimization, overview
-
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APPLICATION
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
medicine
-
the low substrate specificity of the enzyme is used advantageously for synthesis of nucleoside analogs, some of them of medical interest
synthesis
synthesis
-
development of a practical method for enzymatic synthesis of deoxyguanosine by the combination of transglycosylation with NdRT-II from thymidine to a 2-amino-6-substituted purine, and the hydrolysis reaction with bacterial adenosine deaminase
synthesis
-
useful as biocatalyst, immobilization on calcium alginate or calcium pectate of the enzyme increases the 2'-deoxynucleoside synthesis effiency of the organism
synthesis
-
useful as biocatalyst, immobilization on calcium alginate or calcium pectate of the enzyme increases the 2'-deoxynucleoside synthesis effiency of the organism
synthesis
-
useful as biocatalyst, immobilization on calcium alginate or calcium pectate of the enzyme increases the 2'-deoxynucleoside synthesis effiency of the organism
synthesis
-
useful as biocatalyst, immobilization on calcium alginate or calcium pectate of the enzyme increases the 2'-deoxynucleoside synthesis effiency of the organism
synthesis
-
covalent attachment of recombinant Lactobacillus reuteri 2'-deoxyribosyltransferase to Sepabeads EC-EP303. The immobilized enzyme retains 50% of its maximal activity after 17.9 h at 60°C, and 96% activity is observed after storage at 40°C for 110 h. Immobilized enzyme can be recycled for 26 consecutive batch reactions in the synthesis of 2,6-diaminopurine-2'-deoxyriboside with negligible loss of catalytic activity and can be employed in the enzymatic synthesis of different natural and therapeutic nucleosides such as 5-ethyl-2'-deoxyuridine and 5-trifluorothymidine
synthesis
-
immobilization of 2'-deoxyribosyltransferase from Lactobacillus reuteri on Sepabeads. Immobilized enzyme activity is enhanced 1.21.4fold at 20% of methanol, ethanol, 2-propanol, diethylene glycol, and acetone, and at 10% and 30% acetonitrile. Highest increased activity is also obtained in presence of 20% acetonitrile. Immobilized enzyme is successfully used in the synthesis of 2'-deoxyxanthosine and 2'-deoxyguanosine using 2'-deoxyuridine as sugar donor and the corresponding poor water-soluble base in the presence of 30% of methanol, ethanol, 2-propanol, ethylene glycol, acetonitrile, and DMSO, giving high nucleoside yields at 4 h
synthesis
-
useful as biocatalyst, immobilization on calcium alginate or calcium pectate of the enzyme increases the 2'-deoxynucleoside synthesis effiency of the organism
-
synthesis
-
useful as biocatalyst, immobilization on calcium alginate or calcium pectate of the enzyme increases the 2'-deoxynucleoside synthesis effiency of the organism
-
synthesis
-
useful as biocatalyst, immobilization on calcium alginate or calcium pectate of the enzyme increases the 2'-deoxynucleoside synthesis effiency of the organism
-
synthesis
-
useful as biocatalyst, immobilization on calcium alginate or calcium pectate of the enzyme increases the 2'-deoxynucleoside synthesis effiency of the organism
-
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LINKS TO OTHER DATABASES (specific for EC-Number 2.4.2.6)
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