EC Number   |
|---|
   2.4.2.36 | 0.2 microm crossflow microfiltration, concentrated and diafiltered against 10 mM phosphate buffer, pH 7.6, with 30 kDa crossflow ultrafiltration, toxin bound on a S 5/50 GL cation exchange column and eluted with a salt gradient of 10 mM potassium phosphate buffer, pH 7.0 with 1 M NaCl |
| 2.4.2.36 | adsorption on Ni-NTA resin column, washing and elution with buffer containing 500 mM imidazole, dialyzing in 2 steps |
| 2.4.2.36 | exotoxin A |
| 2.4.2.36 | HisTrap column chromatography and Superdex S200 gel filtration |
| 2.4.2.36 | Ni-NTA agarose column chromatography |
| 2.4.2.36 | recombinant catalytic fragment of ExoA, ExoAc |
