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Search Purification (Commentary)

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EC Number Purification (Commentary) Reference
Show all pathways known for 2.4.1.227Display the reaction diagram Show all sequences 2.4.1.227- 488971, 758570
Show all pathways known for 2.4.1.227Display the reaction diagram Show all sequences 2.4.1.227native MurG is extracted from inner membrane vesicles of Escherichia coli cells, His-tagged MurG is purified by Ni-affinity chromatography using His-bind resin 676084
Show all pathways known for 2.4.1.227Display the reaction diagram Show all sequences 2.4.1.227Ni2+ affinity column chromatography 703764
Show all pathways known for 2.4.1.227Display the reaction diagram Show all sequences 2.4.1.227overexpression in Escherichia coli with His-tag 658448
Show all pathways known for 2.4.1.227Display the reaction diagram Show all sequences 2.4.1.227recombinant His6-tagged enzyme from Escherichia coli strain C43 by nickel affinity chromatography 721824
Show all pathways known for 2.4.1.227Display the reaction diagram Show all sequences 2.4.1.227using Ni2+-nitrilotriacetate-agarose 691120
Show all pathways known for 2.4.1.227Display the reaction diagram Show all sequences 2.4.1.227when overexpressed, enzyme copurifies with lipid vesicles deriving from intracellular vesicular mebranes 659026
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