Information on EC 2.4.1.227 - undecaprenyldiphospho-muramoylpentapeptide beta-N-acetylglucosaminyltransferase

Lipid I acetylglucosaminyltransferase

-

MsmegMurG

MurG

15 entries

MurG glycosyltransferase

-

MurG transferase

peptidoglycan glycosyltransferase

proteins, gene murG

-

translocase II

-

UDP-acetylglucosamine-acetylmuramoylpentapeptide pyrophospholipid acetylglucosaminyltransferase

-

UDP-N-acetyl-alpha-D-glucosamine + Mur2Ac(oyl-L-Ala-gamma-D-Glu-L-Lys-D-Ala-D-Ala)-diphosphoundecaprenol = UDP + beta-D-GlcNAc-(1->4)-Mur2Ac(oyl-L-Ala-gamma-D-Glu-L-Lys-D-Ala-D-Ala)-diphosphoundecaprenol

transfer of alpha-N-acetylglucosamine

peptidoglycan biosynthesis

BRENDA

KEGG

Peptidoglycan biosynthesis

MetaCyc

peptidoglycan biosynthesis I (meso-diaminopimelate containing), peptidoglycan biosynthesis II (staphylococci), peptidoglycan biosynthesis III (mycobacteria), peptidoglycan biosynthesis IV (Enterococcus faecium), peptidoglycan biosynthesis V (beta-lactam resistance)

UDP-N-acetyl-D-glucosamine:N-acetyl-alpha-D-muramyl(oyl-L-Ala-gamma-D-Glu-L-Lys-D-Ala-D-Ala)-diphosphoundecaprenol 4-beta-N-acetylglucosaminlytransferase

The enzyme also works when the lysine residue is replaced by meso-2,6-diaminoheptanedioate (meso-2,6-diaminopimelate, A2pm) combined with adjacent residues through its L-centre, as it is in Gram-negative and some Gram-positive organisms. The undecaprenol involved is ditrans,octacis-undecaprenol (for definitions, {iupac/misc/prenol#p6::click here}).

60976-26-3

-

dUDP-N-acetyl-alpha-D-glucosamine + Mur2Ac(oyl-L-Ala-gamma-D-Glu-L-Lys-D-Ala-D-Ala)-diphosphoundecaprenol

?

-

488977

-

?

UDP-N-acetyl-alpha-D-glucosamine + biotinylated citronellyl-lipid I

?

-

-

?

UDP-N-acetyl-alpha-D-glucosamine + C55-lipid I-Nepsilon-C6-dansyl thiourea

UDP + C55-lipid II-Nepsilon-C6-dansyl thiourea

UDP-N-acetylglucosamine + biotin-labelled lipid I analogue

?

-

-

?

UDP-N-acetylglucosamine + citronellyl-lipid I

UDP + N-acetylglucosamine-citronellyl-lipid I

UDP-N-acetylglucosamine + lipid I

UDP + lipid II

-

i.e. Mur2Ac(oyl-L-Ala-gamma-D-Glu-L-Lys-D-Ala-D-Ala)-diphosphoundecaprenol

658448, 659026

i.e. N-acetylglucosamine-(1-4)-Mur2Ac(oyl-L-Ala-gamma-D-Glu-L-Lys-D-Ala-D-Ala)-diphosphoundecaprenol

-

?

UDP-N-acetylglucosamine + Mur2Ac(oyl-L-Ala-gamma-D-Glu-L-Lys-D-Ala-D-Ala)-diphospho-(6E,10E,14Z)-2,6,10,14-tetramethyl-hexadeca-2,6,10,14-tetraene

UDP + GlcNAcbeta(1-4)Mur2Ac(oyl-L-Ala-gamma-D-Glu-L-Lys-D-Ala-D-Ala)-diphospho-(6E,10E,14Z)-2,6,10,14-tetramethyl-hexadeca-2,6,10,14-tetraene

-

-

?

UDP-N-acetylglucosamine + Mur2Ac(oyl-L-Ala-gamma-D-Glu-L-Lys-D-Ala-D-Ala)-diphospho-(7S)-2,7-dimethyloct-2-ene

UDP + GlcNAcbeta(1-4)Mur2Ac(oyl-L-Ala-gamma-D-Glu-L-Lys-D-Ala-D-Ala)-diphospho-(7S)-2,7-dimethyloct-2-ene

-

-

?

UDP-N-acetylglucosamine + Mur2Ac(oyl-L-Ala-gamma-D-Glu-L-Lys-D-Ala-D-Ala)-diphospho-p-nitrophenol

UDP + GlcNAcbeta(1-4)Mur2Ac(oyl-L-Ala-gamma-D-Glu-L-Lys-D-Ala-D-Ala)-diphospho-p-nitrophenol

-

-

?

UDP-N-acetylglucosamine + Mur2Ac(oyl-L-Ala-gamma-D-Glu-L-Lys-D-Ala-D-Ala)-diphosphobutane

UDP + GlcNAcbeta(1-4)Mur2Ac(oyl-L-Ala-gamma-D-Glu-L-Lys-D-Ala-D-Ala)-diphosphobutane

-

-

?

UDP-N-acetylglucosamine + Mur2Ac(oyl-L-Ala-gamma-D-Glu-L-Lys-D-Ala-D-Ala)-diphosphododecane

UDP + GlcNAcbeta(1-4)Mur2Ac(oyl-L-Ala-gamma-D-Glu-L-Lys-D-Ala-D-Ala)-diphosphododecane

-

-

?

UDP-N-acetylglucosamine + Mur2Ac(oyl-L-Ala-gamma-D-Glu-L-Lys-D-Ala-D-Ala)-diphosphoicosane

UDP + GlcNAcbeta(1-4)Mur2Ac(oyl-L-Ala-gamma-D-Glu-L-Lys-D-Ala-D-Ala)-diphosphoicosane

-

-

?

UDP-N-acetylglucosamine + Mur2Ac(oyl-L-Ala-gamma-D-Glu-L-Lys-D-Ala-D-Ala)-diphosphotetradecane

UDP + GlcNAcbeta(1-4)Mur2Ac(oyl-L-Ala-gamma-D-Glu-L-Lys-D-Ala-D-Ala)-diphosphotetradecane

-

-

?

UDP-N-acetylglucosamine + Mur2Ac(oyl-L-Ala-gamma-D-Glu-L-Lys-D-Ala-D-Ala)-diphosphoundecaprenol

UDP + GlcNAc-(1-4)-Mur2Ac(oyl-L-Ala-gamma-D-Glu-L-Lys-D-Ala-D-Ala)-diphosphoundecaprenol

UDP-N-acetylglucosamine + Mur2Ac(oyl-L-Ala-gamma-D-Glu-L-Lys-D-Ala-D-Ala)-diphosphoundecaprenol

UDP + GlcNAc-(1->4)-Mur2Ac(oyl-L-Ala-gamma-D-Glu-L-Lys-D-Ala-D-Ala)-diphosphoundecaprenol

UDP-N-acetylglucosamine + Mur2Ac(oyl-L-Ala-gamma-D-Glu-L-Lys-D-Ala-D-Ala)-diphosphoundecaprenol

UDP + N-acetylglucosamine-(1-4)-Mur2Ac(oyl-L-Ala-gamma-D-Glu-L-Lys-D-Ala-D-Ala)-diphosphoundecaprenol

UDP-N-acetylglucosamine + MurNAc(Nepsilon-dansylpentapeptide)-pyrophosphoryl (R,S)-alpha-dihydrodecaprenol

UDP + N-acetylglucosamine-MurNAc(Nepsilon-dansylpentapeptide)-pyrophosphoryl (R,S)-alpha-dihydrodecaprenol

-

very poor substrate

-

?

UDP-N-acetylglucosamine + MurNAc(Nepsilon-dansylpentapeptide)-pyrophosphoryl (R,S)-alpha-dihydroheptaprenol

UDP + N-acetylglucosamine-MurNAc(Nepsilon-dansylpentapeptide)-pyrophosphoryl (R,S)-alpha-dihydroheptaprenol

-

-

r

UDP-N-acetylglucosamine + MurNAc(Neta-dansylpentapeptide)-diphosphoryl (R,S-alpha-dihydroheptaprenol)

?

-

-

?

UDP-N-acetylglucosamine + synthetic substrate analogue 2

?

-

synthetic substrate analogue 2: a 10 carbon citronellyl derivative

-

?

UDP-N-acetylglucosamine + synthetic substrate analogue 7

?

-

synthetic substrate analogue 7: a derivative containing a 20 carbon chain with a cis-allylic double bond

-

?

additional information

?

-

12 entries

UDP-N-acetylglucosamine + Mur2Ac(oyl-L-Ala-gamma-D-Glu-L-Lys-D-Ala-D-Ala)-diphosphoundecaprenol

UDP + GlcNAc-(1-4)-Mur2Ac(oyl-L-Ala-gamma-D-Glu-L-Lys-D-Ala-D-Ala)-diphosphoundecaprenol

UDP-N-acetylglucosamine + Mur2Ac(oyl-L-Ala-gamma-D-Glu-L-Lys-D-Ala-D-Ala)-diphosphoundecaprenol

UDP + GlcNAc-(1->4)-Mur2Ac(oyl-L-Ala-gamma-D-Glu-L-Lys-D-Ala-D-Ala)-diphosphoundecaprenol

UDP-N-acetylglucosamine + Mur2Ac(oyl-L-Ala-gamma-D-Glu-L-Lys-D-Ala-D-Ala)-diphosphoundecaprenol

UDP + N-acetylglucosamine-(1-4)-Mur2Ac(oyl-L-Ala-gamma-D-Glu-L-Lys-D-Ala-D-Ala)-diphosphoundecaprenol

additional information

?

-

-

role of enzyme in peptidoglycan biosynthesis pathway

659026

-

?

Mg2+

-

activates

Mn2+

-

activating compound

(1R,3R,3aS,6aR)-1-{[2-({[(2R,3R,4R,5R)-5-(2,4-dioxo-3,4-dihydropyrimidin-1(2H)-yl)-3,4-dihydroxytetrahydrofuran-2-yl] methyl}amino)-2-oxoethyl]carbamoyl}-3-(2-methoxyphenyl)-1-methyl-4,6-dioxo-5-phenyloctahydropyrrolo[3,4-c]pyrrol-2-ium

-

(1R,3S,3aR,6aS)-3-((S)-1,2-dihydroxyethyl)-N-(2-((((2R,3S,4R,5R)-5-(2,4-dioxo-3,4-dihydropyrimidin-1(2H)-yl)-3,4-dihydroxytetrahydrofuran-2-yl)methyl)amino)-2-oxoethyl)-1-methyl-4,6-dioxo-5-phenyloctahydropyrrolo[3,4-c]pyrrole-1-carboxamide

-

(1R,3S,3aR,6aS)-3-(2,3-dimethoxyphenyl)-N-[2-({[(2R,3S,4R,5R)-5-(2,4-dioxo-3,4-dihydropyrimidin-1(2H)-yl)-3,4-dihydroxytetrahydrofuran-2-yl]methyl}amino)-2-oxoethyl]-1-methyl-4,6-dioxo-5-phenyloctahydropyrrolo[3,4-c]pyrrole-1-carboxamide

-

(1R,3S,3aR,6aS)-N-[2-({[(2R,3S,4R,5R)-5-(2,4-dioxo-3,4-dihydropyrimidin-1(2H)-yl)-3,4-dihydroxytetrahydrofuran-2-yl]methyl}amino)-2-oxoethyl]-1-methyl-4,6-dioxo-3,5-diphenyloctahydropyrrolo[3,4-c]pyrrole-1-carboxamide

-

(1R,3S,3aR,6aS)-N-[2-({[(2R,3S,4R,5R)-5-(2,4-dioxo-3,4-dihydropyrimidin-1(2H)-yl)-3,4-dihydroxytetrahydrofuran-2-yl]methyl}amino)-2-oxoethyl]-3-(2-methoxyphenyl)-1-methyl-4,6-dioxo-5-phenyloctahydropyrrolo[3,4-c]pyrrole-1-carboxamide

-

(1R,3S,3aR,6aS)-N-[2-({[(2R,3S,4R,5R)-5-(2,4-dioxo-3,4-dihydropyrimidin-1(2H)-yl)-3,4-dihydroxytetrahydrofuran-2-yl]methyl}amino)-2-oxoethyl]-3-(2-methoxyphenyl)-2-methyl-4,6-dioxo-5-phenyloctahydropyrrolo[3,4-c]pyrrole-1-carboxamide

-

(2S)-N-{[(2R,3S,4R,5R)-5-(2,4-dioxo-3,4-dihydropyrimidin-1(2H)-yl)-3,4-dihydroxytetrahydrofuran-2-yl]methyl}-1-{[(1R,3S,3aR,6aS)-3-(2-methoxyphenyl)-1-methyl-4,6-dioxo-5-phenyloctahydropyrrolo[3,4-c]pyrrol-1-yl]carbonyl}pyrrolidine-2-carboxamide

-

(2S)-N-{[(2R,3S,4R,5R)-5-(2,4-dioxo-3,4-dihydropyrimidin-1(2H)-yl)-3,4-dihydroxytetrahydrofuran-2-yl]methyl}-1-{[(1R,3S,3aR,6aS)-5-ethyl-3-(2-methoxyphenyl)-1-methyl-4,6-dioxooctahydropyrrolo[3,4-c]pyrrol-1-yl]carbonyl}pyrrolidine-2-carboxamide

-

(4R,4aR,5aS,6R,12aS)-4-(dimethylamino)-1,5,10,12,12a-pentahydroxy-6-methyl-3,11-dioxo-3,4,4a,5,5a,6,11,12a- octahydrotetracene-2-carboxamide

-

(5E)-5-[(4-tert-butylphenyl)methylidene]-3-[(4-methylpiperidin-1-yl)methyl]-2-sulfanylidene-1,3-thiazolidin-4-one

-

-

(5E)-5-[(5-bromofuran-2-yl)methylidene]-1-(4-chlorophenyl)-1,3-diazinane-2,4,6-trione

-

-

({2-[3-(4-methoxybenzyl)-6-(methoxycarbonyl)-3,4,6,7-tetrahydro-5H-imidazo[4,5-c]pyridin-5-yl]-2-oxoethyl}sulfanyl)acetic acid

-

2-(carboxyethynyl)-3,4-dihydroxybenzoic acid

-

2-(phosphonooxy)butanoic acid

-

2-[(5Z)-5-(1-benzyl-5-bromo-2-oxo-1,2-dihydro-3H-indol-3-ylidene)-4-oxo-2-sulfanylidene-1,3-thiazolidin-3-yl]ethane-1-sulfonic acid

-

-

3-[[(3E)-3-[[5-(4-bromophenyl)furan-2-yl]methylidene]-2-oxo-5-phenyl-2,3-dihydro-1H-pyrrol-1-yl]methyl]benzoic acid

-

-

4-{[(1S,11aS)-5,11-dioxo-7-phenyl-2,3,5,10,11,11a-hexahydro-1H-pyrrolo[2,1-c][1,4]benzodiazepin-1-yl]amino}-4-oxobutanoic acid

-

5'-({[(2R,3R,3aR,6aR)-3-carboxy-2-(2,3-dihydroxypropyl)-6a-methyl-4,6-dioxohexahydropyrrolo[3,4-b]pyrrol-5(1H)-yl]acetyl}amino)-5'-deoxyuridine

-

5'-({[(2S,3R,3aR,6aR)-3-carboxy-2-(2-methoxyphenyl)-6a-methyl-4,6-dioxohexahydropyrrolo[3,4-b]pyrrol-5(1H)-yl]acetyl}amino)-5'-deoxyuridine

-

5'-({[(2S,3R,3aS,6aR)-3-carboxy-2-(2,3-dimethoxyphenyl)-6a-methyl-4,6-dioxohexahydropyrrolo[3,4-b]pyrrol-5(1H)-yl]acetyl}amino)-5'-deoxyuridine

-

5'-({[(2S,3R,3aS,6aR)-3-carboxy-2-(2-methoxyphenyl)-6a-methyl-4,6-dioxohexahydropyrrolo[3,4-b]pyrrol-5(1H)-yl]acetyl}amino)-5'-deoxyuridine

-

5'-({[(2S,3R,3aS,6aR)-3-carboxy-2-(3,4-dimethoxyphenyl)-6a-methyl-4,6-dioxohexahydropyrrolo[3,4-b]pyrrol-5(1H)-yl]acetyl}amino)-5'-deoxyuridine

-

5'-({[(2S,3R,3aS,6aR)-3-carboxy-6a-methyl-4,6-dioxo-2-phenylhexahydropyrrolo[3,4-b]pyrrol-5(1H)-yl]acetyl}amino)-5'-deoxyuridine

-

5'-deoxy-5'-[(N-{[(2R,3R,4R,5R)-3-(methoxycarbonyl)-5-(2-methoxyphenyl)-2,4-dimethylpyrrolidinium-2-yl]carbonyl}glycyl) amino]uridine

-

5'-O-(3-{[(1R,3S,3aR,6aS)-3-(2-methoxyphenyl)-1-methyl-4,6-dioxo-5-phenyloctahydropyrrolo[3,4-c]pyrrol-1-yl]methoxy}-3-oxopropanoyl)uridine

-

5'-O-(4-{[(1R,3S,3aR,6aS)-3-(2-methoxyphenyl)-1-methyl-4,6-dioxo-5-phenyloctahydropyrrolo[3,4-c]pyrrol-1-yl]methoxy}-4-oxobutanoyl)uridine

-

5'-[(1-{[(2R,3R,4R,5S)-3,4-bis(methoxycarbonyl)-5-(2-methoxyphenyl)-2-methylpyrrolidin-2-yl]carbonyl}-L-prolyl)amino]-5'-deoxyuridine

-

5'-[(1-{[(2R,3S,4R,5S)-3,4-bis(methoxycarbonyl)-5-(2-methoxyphenyl)-2-methylpyrrolidin-2-yl]carbonyl}-L-prolyl)amino]-5'-deoxyuridine

-

5'-{[(3S,4R,5S)-3,4-bis(methoxycarbonyl)-5-(2-methoxyphenyl)-2-methyl-D-prolylglycyl]amino}-5'-deoxyuridine

-

5'-{[(3S5'-{[(3S,4R,5S)-3,4-bis(methoxycarbonyl)-2-methyl-5-phenyl-D-prolylglycyl]amino}-5'-deoxyuridine,4R,5S)-3,4-bis(methoxycarbonyl)-2-methyl-5-phenyl-D-prolylglycyl]amino}-5'-deoxyuridine

-

5-(3-[(E)-[1-(3-chlorophenyl)-2,4,6-trioxo-1,3-diazinan-5-ylidene]methyl]-2,5-dimethyl-1H-pyrrol-1-yl)benzene-1,3-dicarboxylic acid

-

-

5-([3-[2-(4-tert-butylphenoxy)ethoxy]phenyl]methylidene)-2-sulfanylidene-1,3-diazinane-4,6-dione

-

-

cephalosporin C

-

50% inhibition at 0.014 mg/ml

EDTA

-

methanol

-

Moenomycin

-

50% inhibition at 0.0106 mM

murgocil

Staphylococcus aureus

-

a highly bioactive staphylococcal-specific inhibitor of intracellular membrane-associated enzyme MurG

735347

N-({6-[(4-cyano-2-fluorobenzyl)oxy]naphthalen-2-yl}sulfonyl)-D-glutamic acid

-

N-p-chlorobiphenyl-vancomycin

-

50% inhibition at 0.00125 mM

NG-p-chlorobiphenyl-vancomycin

-

50% inhibition at 0.0214 mM

Ni2+

-

nikkomycin Z

nisin

-

50% inhibition at 0.016 mg/ml

657559

Polyoxin D

PyDT-1

-

-

ramoplanin

ramoplanin A2

-

ristocetin A

-

ToXa-1

-

-

trifluoroethanol

-

tunicamycin

UDP

Vancomycin

ZINC09186673

Acinetobacter baumannii

B0V9F5

-

759512

-

ZINC09956120

Acinetobacter baumannii

B0V9F5

-

759512

-

Zn2+

-

[(5E)-5-[(3-bromo-5-chloro-2-hydroxyphenyl)methylidene]-4-oxo-2-sulfanylidene-1,3-thiazolidin-3-yl]acetic acid

-

-

[1-hydroxy-2-(pyridin-3-yl)ethane-1,1-diyl]bis(phosphonic acid)

-

additional information

-

Ca2+

-

cardiolipin

-

the activity of MurG is increased in the presence of cardiolipin

dimethyl sulfoxide

-

DMSO

-

high concentration (35%) of dimethylsulfoxide is necessary for maximal enzyme activity

Mg2+

-

488971, 488973, 488974, 488976, 488978

1.5

2'-dUDP-N-acetyl-alpha-D-glucosamine

-

488977

0.0028 - 0.037

biotin-labelled lipid I analogue

-

0.036 - 0.044

biotinylated citronellyl-lipid I

0.053

Mur2Ac(oyl-L-Ala-gamma-D-Glu-L-Lys-D-Ala-D-Ala)-diphospho-(6E,10E,14Z)-2,6,10,14-tetramethyl-hexadeca-2,6,10,14-tetraene

-

pH 7.9, 37°C

0.047

Mur2Ac(oyl-L-Ala-gamma-D-Glu-L-Lys-D-Ala-D-Ala)-diphospho-(7S)-2,7-dimethyloct-2-ene

-

pH 7.9, 37°C

0.024

Mur2Ac(oyl-L-Ala-gamma-D-Glu-L-Lys-D-Ala-D-Ala)-diphospho-p-nitrophenol

-

pH 7.9, 37°C

0.022

Mur2Ac(oyl-L-Ala-gamma-D-Glu-L-Lys-D-Ala-D-Ala)-diphosphobutane

-

pH 7.9, 37°C

0.029

Mur2Ac(oyl-L-Ala-gamma-D-Glu-L-Lys-D-Ala-D-Ala)-diphosphododecane

-

pH 7.9, 37°C

0.052

Mur2Ac(oyl-L-Ala-gamma-D-Glu-L-Lys-D-Ala-D-Ala)-diphosphoicosane

-

pH 7.9, 37°C

0.039

Mur2Ac(oyl-L-Ala-gamma-D-Glu-L-Lys-D-Ala-D-Ala)-diphosphotetradecane

-

pH 7.9, 37°C

0.02

Mur2Ac(oyl-L-Ala-gamma-D-Glu-L-Lys-D-Ala-D-Ala)-diphosphoundecaprenol

-

pH 7.9, 37°C

0.0028

MurNAc(Nepsilon-dansylpentapeptide)-pyrophosphoryl (R,S)-alpha-dihydroheptaprenol

-

0.0028 - 0.011

MurNAc(Neta-dansylpentapeptide)-diphosphoryl (R,S-alpha-dihydroheptaprenol)

9 entries

0.553

synthetic substrate analogue 2

-

10 carbon citronellyl derivative

-

0.053

synthetic substrate analogue 7

-

0.0098 - 1.03

UDP-GlcNAc

10 entries

0.045 - 0.15

UDP-N-acetylglucosamine

6 entries

additional information

-

continous fluorescence coupled enzyme assay method

-

0.123 - 0.317

biotinylated citronellyl-lipid I

0.933

MurNAc(Nepsilon-dansylpentapeptide)-pyrophosphoryl (R,S)-alpha-dihydroheptaprenol

-

2.23

synthetic substrate analogue 2

-

10 carbon citronellyl derivative

-

14

synthetic substrate analogue 7

-

0.001 - 0.72

UDP-GlcNAc

9 entries

0.27 - 0.93

UDP-N-acetylglucosamine

0.0014

(5E)-5-[(4-tert-butylphenyl)methylidene]-3-[(4-methylpiperidin-1-yl)methyl]-2-sulfanylidene-1,3-thiazolidin-4-one

Escherichia coli

-

pH 7.9, temperature not specified in the publication

-

0.0064

(5E)-5-[(5-bromofuran-2-yl)methylidene]-1-(4-chlorophenyl)-1,3-diazinane-2,4,6-trione

Escherichia coli

-

pH 7.9, temperature not specified in the publication

-

0.0035

2-[(5Z)-5-(1-benzyl-5-bromo-2-oxo-1,2-dihydro-3H-indol-3-ylidene)-4-oxo-2-sulfanylidene-1,3-thiazolidin-3-yl]ethane-1-sulfonic acid

Escherichia coli

-

pH 7.9, temperature not specified in the publication

-

0.0054

3-[[(3E)-3-[[5-(4-bromophenyl)furan-2-yl]methylidene]-2-oxo-5-phenyl-2,3-dihydro-1H-pyrrol-1-yl]methyl]benzoic acid

Escherichia coli

-

pH 7.9, temperature not specified in the publication

-

0.0014

5-(3-[(E)-[1-(3-chlorophenyl)-2,4,6-trioxo-1,3-diazinan-5-ylidene]methyl]-2,5-dimethyl-1H-pyrrol-1-yl)benzene-1,3-dicarboxylic acid

Escherichia coli

-

pH 7.9, temperature not specified in the publication

-

0.004

5-([3-[2-(4-tert-butylphenoxy)ethoxy]phenyl]methylidene)-2-sulfanylidene-1,3-diazinane-4,6-dione

Escherichia coli

-

pH 7.9, temperature not specified in the publication

-

0.0087 - 0.0095

nikkomycin Z

0.0508 - 0.055

Polyoxin D

0.0035

PyDT-1

Mycolicibacterium smegmatis

-

pH 8.0, 37°C

-

0.0224 - 0.0235

ramoplanin A2

-

0.00096 - 0.0014

ristocetin A

-

0.002

ToXa-1

Mycolicibacterium smegmatis

-

pH 8.0, 37°C

-

0.0034

[(5E)-5-[(3-bromo-5-chloro-2-hydroxyphenyl)methylidene]-4-oxo-2-sulfanylidene-1,3-thiazolidin-3-yl]acetic acid

Escherichia coli

-

pH 7.9, temperature not specified in the publication

-

0.000000096

-

thermosensitive murG mutant strain GS58, 40°C

0.000000336

-

thermosensitive murG mutant strain GS58, 30°C

0.0000016

-

parental strain, 30°C

0.000002

-

parental strain, 40°C

0.0000064

-

murG gene under the control of the lac promotor

0.0000141

-

0.000019

mutant N127A

0.000063

mutant E268A

0.00026

mutant H18A

0.00028

mutant Q288A

0.00043

mutant E125A

0.00053

mutant T15A

0.00067

mutant H124A

0.0023

mutant R260A

0.0025

mutant S191A

0.0064

mutant N291A

0.0135

mutant N198A

0.071

mutant Y105A

0.37

wild type enzyme

additional information

7.5

8.3

Acinetobacter baumannii AYE

-

Hepes buffer

7.5 - 9.5

-

22

-

assay at room temperature

25

35

37

Acinetobacter baumannii

-

759512

B0V9F5

SwissProt

-

SwissProt

-

-

-

Clostridioides difficile R20291

-

-

-

Escherichia coli OV58-pUG18

10 entries

-

488972

-

Hydrogenivirga sp. 128-5-R1-1

-

UniProt

gene murG

-

-

Mycolicibacterium smegmatis ATCC 607

-

-

-

Pseudomonas aeruginosa

-

723716

Q9HW01

UniProt

Staphylococcus aureus

methicillin-resistant

-

-

during exponential growth, MurG localizes to septa of Bacillus subtilis, a zone of active peptidoglycan synthesis, and its N-terminal amphipathic helix is dispensable

-

-

additional information

-

MurG becomes polarly localized when expressed at high cellular concentrations, only at levels that saturate MurGs cellular requirement for growth, the polar MurG is not active

-

722525

drug target

malfunction

-

point mutations in a MurG helical causes severe sporulation defects, but does not affect localization nor cause detectable defects during exponential growth. In strains in which the cardiolipin-synthesizing genes are deleted, MurG levels are diminished at the forespore, but MurG localization during sporulation is rescued by external addition of purified cardiolipin. During sporulation, lack of MurG localization heavily affects engulfment dynamics and sporulation efficiency, indicating a defect in MurG enzymatic activity linked to its diffuse localization

Pseudomonas aeruginosa PAO1

metabolism

5 entries

physiological function

5 entries

additional information

5 entries

3s2u, download, 3D-view

SCOPe (3s2u)

CATH (3s2u)

Q9HW01

37640

-

calculated from the nucleotide sequence, gel filtration

37800

38000

43000

determined by SDS-PAGE

76000

-

gel filtration

?

-

x * 38000, SDS-PAGE

658448, 659026

dimer

-

2 * 38000, SDS-PAGE

additional information

hanging-drop vapor-diffusion method, X-ray structure

488977

complex of MurG with UDP-GlcNAc, X-ray diffraction structure determination and analysis

Pseudomonas aeruginosa

Q9HW01

723716

F77E

-

site-directed mutagenesis, the mutant shows unaltered localization and cell growth comared to wild-type. Lipid II synthesis might be totally abrogated in mutant F77E explaining why the mutant is blocked in septation, cell wall synthesis is necessary for engulfment

S67E

-

site-directed mutagenesis, the mutant shows unaltered localization and cell growth comared to wild-type

V74E

-

site-directed mutagenesis, the mutant shows unaltered localization and cell growth comared to wild-type. Lipid II synthesis might be totally abrogated in mutant V74E explaining why the mutant is blocked in septation, cell wall synthesis is necessary for engulfment

V81E

-

site-directed mutagenesis, the mutant shows unaltered localization and cell growth comared to wild-type

E125A

amino acid substitution for evaluation of its role in the active site of MurG

E268A

H124A

amino acid substitution for evaluation of its role in the active site of MurG

H18A

amino acid substitution for evaluation of its role in the active site of MurG

L79E/F82E

-

site-directed mutagenesis, the mutant does not bind anionic phospholipids but can localize to the cell poles

722525

N127A

amino acid substitution for evaluation of its role in the active site of MurG

N134A

amino acid substitution for evaluation of its role in the active site of MurG

N198A

amino acid substitution for evaluation of its role in the active site of MurG

N291A

amino acid substitution for evaluation of its role in the active site of MurG

Q288A

amino acid substitution for evaluation of its role in the active site of MurG

R260A

amino acid substitution for evaluation of its role in the active site of MurG

S191A

amino acid substitution for evaluation of its role in the active site of MurG

T15A

amino acid substitution for evaluation of its role in the active site of MurG

Y105A

amino acid substitution for evaluation of its role in the active site of MurG

additional information

-

none of the point mutations shows lethality and cells expressing MurG-GFP point mutants grow normally in absence of the wild-type murG allele

additon of 20% glycerol required for storage at -20°C

-

-70°C

-80°C

-

4°C, stable for at least one month

-

-

native MurG is extracted from inner membrane vesicles of Escherichia coli cells, His-tagged MurG is purified by Ni-affinity chromatography using His-bind resin

-

676084

Ni2+ affinity column chromatography

-

overexpression in Escherichia coli with His-tag

-

recombinant His6-tagged enzyme from Escherichia coli strain C43 by nickel affinity chromatography

-

using Ni2+-nitrilotriacetate-agarose

when overexpressed, enzyme copurifies with lipid vesicles deriving from intracellular vesicular mebranes

-

659026

expressed in Escherichia coli cells BL21-DE3-pLysS

gene murG, expression as His6-tagged enzyme in Escherichia coli strain C43

-

gene murG, expression of C-terminal YFP-tagged enzyme in a murG-deficient strain of Bacillus subtilis

-

into the vector pQE60 for expression of MurG in Escherichia coli JM109 cells

MurG can be displayed in its active form on phage. Cloned from a pET-21a expression vector (Novagen) into the pFAB5cHis.TT.HUI phagemid vector as a fusion to the 3’-end of a pelB leader sequence and the 5’-end of a truncated gIII

-

673085

the coding sequence is amplified from the Escherichia coli K12 chromosome and cloned for expression of His-tagged MurG in JM109 cells

-

676084

the murG gene is cloned with an N-terminal His6 tag into a pET33b vector. The plasmid is transformed and expressed in Escherichia coli NiCo21(DE3) competent cells

-