EC Number |
---|
2.4.1.14 | - |
2.4.1.14 | complete separation from sucrose synthetase |
2.4.1.14 | DE52 anion exchange cellulose chromatography and His tag affinity column chromatography |
2.4.1.14 | enzyme becomes extremely unstable during the course of purification |
2.4.1.14 | gel filtration is carried out in presence of 20% ethylene glycol and 0.2 M KCl to stabilize activity |
2.4.1.14 | in presence of 2-mercaptoethanol and a phenol absorbing agent under N2 |
2.4.1.14 | native enzyme partially from plant tissue by ammonium sulfate fractionation and dialysis |
2.4.1.14 | native isozymes partially by anion exchange chromatography |
2.4.1.14 | partial |
2.4.1.14 | partial, at least 3 additional proteins could not be removed |