EC Number |
---|
2.3.2.5 | - |
2.3.2.5 | chelating Sepharose column chromatography and butyl Sepharose column chromatography |
2.3.2.5 | enzyme is semi-purified from latex crude extracts by ion exchange chromatography on a CM Sephadex column C-50 and gel filtration |
2.3.2.5 | hanging-drop vapour-diffusion method, wild-type and mutant enzymes |
2.3.2.5 | His-tagged version |
2.3.2.5 | hydrophobic interaction chromatography, ion exchange chromatography, gel filtration |
2.3.2.5 | Ni-NTA column chromatography |
2.3.2.5 | Ni-NTA column chromatography and Sephacryl S-100 gel filtration |
2.3.2.5 | of 6His-tagged enzyme expressed in Pichia pastoris and enzyme expressed in Escherichia coli |
2.3.2.5 | partial |