EC Number   |
|---|
   2.3.1.75 | glutathione-Sepharose column chromatography and Superdex S200 gel filtration |
| 2.3.1.75 | HisTrap column chromatography and Superdex S200 gel filtration |
| 2.3.1.75 | Ni2+-affinity column chromatography |
| 2.3.1.75 | partially |
| 2.3.1.75 | recombinant C-terminally His6-tagged enzyme from Escherichia coli strain Rosetta (DE3) by nickel affinity chromatography |
| 2.3.1.75 | recombinant enzyme from Escherichia coli by cation exchange chromatography, hydrophobic interaction chromatography and subsequent anion exchange chromatography or by means of a C-terminal His6 tag |
| 2.3.1.75 | recombinant His-tagged enzyme from Escherichia coli strain C41(DE3) by ultracentrifugation and nickel affinity chromatography |
| 2.3.1.75 | recombinant soluble N-terminally His-tagged enzyme 19.2fold from Escherichia coli strain BL21(DE3) by cation exchange and nickel affinity chromatography |
| 2.3.1.75 | recombinant wild-type and mutant enzymes from Escherichia coli by cation exchange and hydrophobic interaction chromatography, followed by anion exchange chromatography |
