EC Number |
---|
2.3.1.51 | - |
2.3.1.51 | gel filtration and SDS-PAGE, recombinant protein |
2.3.1.51 | gel filtration and SDS-PAGE, recombinant protein, mutant and wild-type |
2.3.1.51 | gel filtration, wild-type and mutant protein |
2.3.1.51 | identification of enzyme using solubilization with CHAPS and column chromatography on Red 120 agarose, Bio-Rad HA HT, 12:0-CoA Sepharose 4B, and Superose 12 |
2.3.1.51 | native enzyme partially by microsome preparation |
2.3.1.51 | partial |
2.3.1.51 | partial, separated from EC 2.3.1.15 using Triton X-100, the enzyme is not solubilized by Triton X-100 or cholate |
2.3.1.51 | partially purified, gel filtration |
2.3.1.51 | recombinant active His6-tagged enzyme from Escherichia coli strain C43(DE3) membrane micells by ultracentrifugation, detergent (CYMAL-6) solubilization, nickel affinity chromatography, and ultrafiltration, to homogeneity. The CYMAL-6-solubilized enzyme is active, while a DDM-solubilized enzyme is inactive after purification |