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Search Purification (Commentary)

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EC Number Purification (Commentary)
Show all pathways known for 2.3.1.51Display the word mapDisplay the reaction diagram Show all sequences 2.3.1.51-
Show all pathways known for 2.3.1.51Display the word mapDisplay the reaction diagram Show all sequences 2.3.1.51gel filtration and SDS-PAGE, recombinant protein
Show all pathways known for 2.3.1.51Display the word mapDisplay the reaction diagram Show all sequences 2.3.1.51gel filtration and SDS-PAGE, recombinant protein, mutant and wild-type
Show all pathways known for 2.3.1.51Display the word mapDisplay the reaction diagram Show all sequences 2.3.1.51gel filtration, wild-type and mutant protein
Show all pathways known for 2.3.1.51Display the word mapDisplay the reaction diagram Show all sequences 2.3.1.51identification of enzyme using solubilization with CHAPS and column chromatography on Red 120 agarose, Bio-Rad HA HT, 12:0-CoA Sepharose 4B, and Superose 12
Show all pathways known for 2.3.1.51Display the word mapDisplay the reaction diagram Show all sequences 2.3.1.51native enzyme partially by microsome preparation
Show all pathways known for 2.3.1.51Display the word mapDisplay the reaction diagram Show all sequences 2.3.1.51partial
Show all pathways known for 2.3.1.51Display the word mapDisplay the reaction diagram Show all sequences 2.3.1.51partial, separated from EC 2.3.1.15 using Triton X-100, the enzyme is not solubilized by Triton X-100 or cholate
Show all pathways known for 2.3.1.51Display the word mapDisplay the reaction diagram Show all sequences 2.3.1.51partially purified, gel filtration
Show all pathways known for 2.3.1.51Display the word mapDisplay the reaction diagram Show all sequences 2.3.1.51recombinant active His6-tagged enzyme from Escherichia coli strain C43(DE3) membrane micells by ultracentrifugation, detergent (CYMAL-6) solubilization, nickel affinity chromatography, and ultrafiltration, to homogeneity. The CYMAL-6-solubilized enzyme is active, while a DDM-solubilized enzyme is inactive after purification
Results 1 - 10 of 16 > >>