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Search Purification (Commentary)

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EC Number Purification (Commentary) Reference
Display the word mapDisplay the reaction diagram Show all sequences 1.4.1.20- 349633, 349634, 349636, 349637, 349638, 349640, 349650, 349652
Display the word mapDisplay the reaction diagram Show all sequences 1.4.1.20partial 349635
Display the word mapDisplay the reaction diagram Show all sequences 1.4.1.20construction of a chimeric enzyme consisting of the N-terminal domain of Thermoactinomyces intermedius phenylalanine dehydrogenase, containing the substrate-binding region and the C-terminal domain of leucine dehydrogenase from Bacillus stearothermophilus containing the NAD+-binding region 349647
Display the word mapDisplay the reaction diagram Show all sequences 1.4.1.20mutant enzyme F124M/V125S/H126I/A127I/A128Y/R129Q 349649
Display the word mapDisplay the reaction diagram Show all sequences 1.4.1.20recombinant PheDH 654620, 655468
Display the word mapDisplay the reaction diagram Show all sequences 1.4.1.20recombinant PheDH, ammonium sulfate, DEAE-Toyopearl, hydroxyapatite, Sephadex G-200 657404
Display the word mapDisplay the reaction diagram Show all sequences 1.4.1.20partial purification by Superdex 200 gel filtration 684247
Display the word mapDisplay the reaction diagram Show all sequences 1.4.1.20Ni-affinity column chromatography 685742
Display the word mapDisplay the reaction diagram Show all sequences 1.4.1.20Ni-affinity and anion exchange column chromatography 686443
Display the word mapDisplay the reaction diagram Show all sequences 1.4.1.20polyethylene glycol and ammonium sulfate aequous two-phase purification system with 9% (w/w) PEG-6000 and 16% (w/w) ammonium sulfate at pH 8.0 687210
Results 1 - 10 of 15 > >>