Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Lysinibacillus sphaericus |
Protein Variants | Comment | Organism |
---|---|---|
N145A | 50% of wild-type kcat with phenylalanine | Lysinibacillus sphaericus |
N145I | 19% of wild-type kcat with phenylalanine | Lysinibacillus sphaericus |
N145L | 12.5% of wild-type kcat with phenylalanine | Lysinibacillus sphaericus |
N145V | 25% of wild-type kcat with phenylalanine | Lysinibacillus sphaericus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.087 | - |
L-phenylalanine | - |
Bacillus badius | |
0.14 | - |
L-isoleucine | 25°C, pH 10.4 | Lysinibacillus sphaericus | |
0.19 | - |
p-hydroxy-phenylpyruvate | 25°C, pH 10.4 | Lysinibacillus sphaericus | |
0.37 | - |
phenylpyruvate | 25°C, pH 10.4 | Lysinibacillus sphaericus | |
0.55 | - |
L-leucine | 25°C, pH 10.4 | Lysinibacillus sphaericus | |
0.79 | - |
L-norleucine | 25°C, pH 10.4 | Lysinibacillus sphaericus | |
1.1 | - |
L-phenylalanine | 25°C, pH 10.4 | Lysinibacillus sphaericus | |
1.3 | - |
L-tyrosine | 25°C, pH 10.4 | Lysinibacillus sphaericus | |
2 | - |
L-valine | 25°C, pH 10.4 | Lysinibacillus sphaericus | |
2.7 | - |
L-methionine | 25°C, pH 10.4 | Lysinibacillus sphaericus | |
3.3 | - |
L-norvaline | 25°C, pH 10.4 | Lysinibacillus sphaericus | |
8.6 | - |
L-tyrosine | - |
Bacillus badius | |
11 | - |
alpha-keto-gamma-methiobutyrate | 25°C, pH 10.4 | Lysinibacillus sphaericus | |
13 | - |
alpha-Ketocaproate | 25°C, pH 10.4 | Lysinibacillus sphaericus | |
13 | - |
DL-alpha-keto-beta-methyl-n-valerate | 25°C, pH 10.4 | Lysinibacillus sphaericus | |
20 | - |
4-methyl-2-oxopentanoate | 25°C, pH 10.4 | Lysinibacillus sphaericus | |
52 | - |
alpha-ketovalerate | 25°C, pH 10.4 | Lysinibacillus sphaericus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
39000 | - |
10 * 39000, SDS-PAGE | Lysinibacillus sphaericus |
380000 | - |
gel filtration | Lysinibacillus sphaericus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-phenylalanine + H2O + NAD+ | Lysinibacillus sphaericus | - |
phenylpyruvate + NH3 + NADH | - |
r | |
L-phenylalanine + H2O + NAD+ | Bacillus badius | - |
phenylpyruvate + NH3 + NADH | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bacillus badius | - |
- |
- |
Lysinibacillus sphaericus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
recombinant PheDH | Lysinibacillus sphaericus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-methyl-2-oxopentanoate + NH3 + NADH + H+ | - |
Lysinibacillus sphaericus | L-leucine + NAD+ + H2O | - |
r | |
L-isoleucine + H2O + NAD+ | - |
Lysinibacillus sphaericus | 3-methyl-2-oxopentanoate + NH3 + NADH | - |
r | |
L-leucine + H2O + NAD+ | - |
Lysinibacillus sphaericus | 3,3-dimethyl-2-oxo-butanoate + NH3 + NADH | - |
r | |
L-methionine + H2O + NAD+ | - |
Lysinibacillus sphaericus | (4-methylsulfanyl)-2-oxobutanoate + NH3 + NADH | - |
r | |
L-norleucine + H2O + NAD+ | - |
Lysinibacillus sphaericus | 2-oxohexanoate + NH3 + NADH | - |
r | |
L-norvaline + H2O + NAD+ | - |
Lysinibacillus sphaericus | 2-oxopentanoate + NH3 + NADH | - |
r | |
L-phenylalanine + H2O + NAD+ | - |
Lysinibacillus sphaericus | phenylpyruvate + NH3 + NADH | - |
r | |
L-phenylalanine + H2O + NAD+ | - |
Bacillus badius | phenylpyruvate + NH3 + NADH | - |
r | |
L-tyrosine + H2O + NAD+ | - |
Lysinibacillus sphaericus | (4-hydroxyphenyl)pyruvate + NH3 + NADH | - |
r | |
L-tyrosine + H2O + NAD+ | - |
Bacillus badius | (4-hydroxyphenyl)pyruvate + NH3 + NADH | - |
r | |
L-valine + H2O + NAD+ | - |
Lysinibacillus sphaericus | 3-methyl-2-oxobutanoate + NH3 + NADH | - |
r |
Subunits | Comment | Organism |
---|---|---|
decamer | 10 * 39000, SDS-PAGE | Lysinibacillus sphaericus |
Synonyms | Comment | Organism |
---|---|---|
PheDH | - |
Lysinibacillus sphaericus |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.14 | - |
L-isoleucine | 25°C, pH 10.4 | Lysinibacillus sphaericus | |
0.7 | - |
L-leucine | 25°C, pH 10.4 | Lysinibacillus sphaericus | |
0.91 | - |
L-valine | 25°C, pH 10.4 | Lysinibacillus sphaericus | |
1.2 | - |
L-methionine | 25°C, pH 10.4 | Lysinibacillus sphaericus | |
1.4 | - |
L-norvaline | 25°C, pH 10.4 | Lysinibacillus sphaericus | |
3.2 | - |
L-norleucine | 25°C, pH 10.4 | Lysinibacillus sphaericus | |
7.2 | - |
DL-alpha-keto-beta-methyl-n-valerate | 25°C, pH 10.4 | Lysinibacillus sphaericus | |
27 | - |
4-methyl-2-oxopentanoate | 25°C, pH 10.4 | Lysinibacillus sphaericus | |
27 | - |
alpha-keto-gamma-methiobutyrate | 25°C, pH 10.4 | Lysinibacillus sphaericus | |
33 | - |
alpha-ketovalerate | 25°C, pH 10.4 | Lysinibacillus sphaericus | |
34 | - |
L-tyrosine | - |
Bacillus badius | |
39 | - |
L-phenylalanine | - |
Bacillus badius | |
51 | - |
alpha-Ketocaproate | 25°C, pH 10.4 | Lysinibacillus sphaericus | |
68 | - |
L-tyrosine | 25°C, pH 10.4 | Lysinibacillus sphaericus | |
68.01 | - |
L-phenylalanine | 25°C, pH 10.4 | Lysinibacillus sphaericus | |
90 | - |
p-hydroxy-phenylpyruvate | 25°C, pH 10.4 | Lysinibacillus sphaericus | |
113 | - |
phenylpyruvate | 25°C, pH 10.4 | Lysinibacillus sphaericus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | - |
Lysinibacillus sphaericus | |
NAD+ | - |
Bacillus badius | |
NADH | - |
Lysinibacillus sphaericus |