EC Number   |
|---|
    1.3.1.3 | - |
| 1.3.1.3 | by Ni-NTA affinity chromatography |
| 1.3.1.3 | cortisone 5beta-reduction (EC 1.3.1.3) and androstenedione reduction (EC 1.3.1.23) are catalyzed by the same catalytic site of a single protein |
| 1.3.1.3 | glutathione-Sepharose column chromatography and Sephacryl-S100 gel filtration |
| 1.3.1.3 | Ni2+-Sepharose column chromatography |
| 1.3.1.3 | nickel-Sepharose affinity column chromatography and Superdex 75 gel filtration |
| 1.3.1.3 | partial |
| 1.3.1.3 | recombinant AKR1D1 enzyme mutant P133R from HEK-293 cells, the other expressed mutants cannot be purified |
| 1.3.1.3 | recombinant GST-tagged enzyme from Escherichia coli by glutathione affinity and anion exchange chromatography, and gel filtration |
| 1.3.1.3 | recombinant protein |
