EC Number |
Reference |
---|
1.2.1.48 | crude enzyme solution is applied to an anionic exchange column and eluted by linearly increasing the NaCl concentration from 0-1.0 M in 50 mM Tris-HCl at pH 7.0 |
697840 |
1.2.1.48 | including chromatography in a Resource Q column, recombinant enzyme from Escherichia coli also purified |
390325 |
1.2.1.48 | metal affinity column chromatography |
762634 |
1.2.1.48 | Ni-NTA column chromatography, Mono Q column chromatography, and Superdex 200 gel filtration |
762884 |
1.2.1.48 | recombinant His6-FADH purified by anion exchange and metal-chelate chromatography, to 90-95% purity |
693389 |
1.2.1.48 | recombinant Strep-tagged enzyme from Escherichia coli by affinity chromatography and gel filtration |
743336 |
1.2.1.48 | Strep-tag affinity chromatography and AMP-Sepharose column chromatography |
725656 |
1.2.1.48 | using chromatography on columns consisting of omega-aminohexyl-agarose and 5'-AMP-Sepharose 4B |
390324 |