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Search Purification (Commentary)

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Results 1 - 8 of 8
EC Number Purification (Commentary) Reference
Display the word mapDisplay the reaction diagram Show all sequences 1.2.1.48crude enzyme solution is applied to an anionic exchange column and eluted by linearly increasing the NaCl concentration from 0-1.0 M in 50 mM Tris-HCl at pH 7.0 697840
Display the word mapDisplay the reaction diagram Show all sequences 1.2.1.48including chromatography in a Resource Q column, recombinant enzyme from Escherichia coli also purified 390325
Display the word mapDisplay the reaction diagram Show all sequences 1.2.1.48metal affinity column chromatography 762634
Display the word mapDisplay the reaction diagram Show all sequences 1.2.1.48Ni-NTA column chromatography, Mono Q column chromatography, and Superdex 200 gel filtration 762884
Display the word mapDisplay the reaction diagram Show all sequences 1.2.1.48recombinant His6-FADH purified by anion exchange and metal-chelate chromatography, to 90-95% purity 693389
Display the word mapDisplay the reaction diagram Show all sequences 1.2.1.48recombinant Strep-tagged enzyme from Escherichia coli by affinity chromatography and gel filtration 743336
Display the word mapDisplay the reaction diagram Show all sequences 1.2.1.48Strep-tag affinity chromatography and AMP-Sepharose column chromatography 725656
Display the word mapDisplay the reaction diagram Show all sequences 1.2.1.48using chromatography on columns consisting of omega-aminohexyl-agarose and 5'-AMP-Sepharose 4B 390324
Results 1 - 8 of 8