EC Number   |
|---|
    1.14.14.154 | - |
| 1.14.14.154 | by Ni2+-NTA affinity chromatography, more than 90% pure |
| 1.14.14.154 | Ni-NTA column chromatography, and gel filtration |
| 1.14.14.154 | purification and reconstitution |
| 1.14.14.154 | recombinant C-terminally His-tagged wild-type and mutant enzymes by nickel affinity chromatography and gel filtration |
| 1.14.14.154 | recombinant enzyme |
| 1.14.14.154 | recombinant enzyme from Escherichia coli strain HMS174 (DE3) by two different steps of anion exchange chromatography and hydroxyapaptite chromatography to homogeneity |
| 1.14.14.154 | recombinant GST-tagged enzyme from Escherichia coli by glutathione affinity chromatography, cleavage of the tag by thrombin, and thrombin elimination by benzamidine affinity chromatography |
| 1.14.14.154 | recombinant His-tagged enzyme from Escherichia coli by nickel affinity chromatography, and anion and cation exchange chromatography |
| 1.14.14.154 | recombinant His-tagged enzyme from Escherichia coli strain BL21 (DE3) by nickel affinity chromatography |
