EC Number   |
|---|
   1.14.11.2 | - |
| 1.14.11.2 | DEAE-Sepharose fast flow anion exchange chromatography, phenyl-Sepharose fast flow column chromatography and Source 15Q anion exchange chromatography |
| 1.14.11.2 | isolation of subunits |
| 1.14.11.2 | isolation of subunits by ion exchange chromatography on DEAE-cellulose in 8 M urea |
| 1.14.11.2 | L-proline affinity chromatography and HiTrap Q Sepharose column chromatography |
| 1.14.11.2 | Ni-NTA agarose gel filtration |
| 1.14.11.2 | Ni-NTA column chromatography and gel filtration |
| 1.14.11.2 | Ni2+-affinity chromatography and HiLoad Superdex200 gel filtration |
| 1.14.11.2 | of the beta-subunit using ammonium sulfate fractionation, chromatography on preimmune IgG column and immunoadsorbent column, and gel filtration |
| 1.14.11.2 | of the recombinant enzyme, using an affinity purification procedure based on the use of a histidine tag in the N terminus of the protein disulfide-isomerase/beta polypeptide |
