EC Number |
---|
2.7.1.160 | partially purified, GST-Tpt1 fusion protein purified by glutahthione agarose chromatography |
2.7.1.160 | purified 28000fold by Orange A Sepharose column, 4fold less pure by streamlined purification procedure |
2.7.1.160 | recombinant His6-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography |
2.7.1.160 | recombinant His6-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography and dialysis |
2.7.1.160 | recombinant wild-type and mutant enzymes from Escherichia coli strain BL21(DE3) by nickel affinity chromatography |
2.7.1.160 | sonication, centrifugation |
2.7.1.160 | Tpt1-His6 50% purified by sonication, immobilized metal ion chromatography, centrifugation |