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HeLa cell 2'-phosphotransferase
-
-
NAD+-dependent 2'-phosphotransferase
-
-
NAD-dependent RNA 2'-phosphotransferase
RNA 2'-phosphotransferase
tRNA 2'-phosphotransferase
yeast 2'-phosphotransferase
-
-
2'-phosphotransferase

-
removes 2'-phosphate from the splice junction of the precursor t-RNA
2'-phosphotransferase
-
-
2'-phosphotransferase
yeasts
-
-
KptA

-
mTPT1

-
-
NAD-dependent RNA 2'-phosphotransferase

-
NAD-dependent RNA 2'-phosphotransferase
-
-
NAD-dependent RNA 2'-phosphotransferase
C0R0E9
-
NAD-dependent RNA 2'-phosphotransferase
C0R0E9
-
-
NAD-dependent RNA 2'-phosphotransferase
-
NAD-dependent RNA 2'-phosphotransferase
-
NAD-dependent RNA 2'-phosphotransferase
-
-
NAD-dependent RNA 2'-phosphotransferase
-
NAD-dependent RNA 2'-phosphotransferase
-
-
NAD-dependent RNA 2'-phosphotransferase
-
NAD-dependent RNA 2'-phosphotransferase
-
-
NAD-dependent RNA 2'-phosphotransferase
-
NAD-dependent RNA 2'-phosphotransferase
-
-
NAD-dependent RNA 2'-phosphotransferase
-
NAD-dependent RNA 2'-phosphotransferase
-
NAD-dependent RNA 2'-phosphotransferase
-
-
NAD-dependent RNA 2'-phosphotransferase
-
NAD-dependent RNA 2'-phosphotransferase
-
-
NAD-dependent RNA 2'-phosphotransferase
-
-
NAD-dependent RNA 2'-phosphotransferase
-
NAD-dependent RNA 2'-phosphotransferase
-
-
RNA 2'-phosphotransferase

-
-
RNA 2'-phosphotransferase
-
RNA 2'-phosphotransferase
-
-
RNA 2'-phosphotransferase
-
-
RNA 2'-phosphotransferase
-
-
RNA 2'-phosphotransferase
C0R0E9
-
RNA 2'-phosphotransferase
C0R0E9
-
-
RNA 2'-phosphotransferase
-
RNA 2'-phosphotransferase
-
RNA 2'-phosphotransferase
-
-
RNA 2'-phosphotransferase
-
RNA 2'-phosphotransferase
-
-
RNA 2'-phosphotransferase
-
RNA 2'-phosphotransferase
-
-
RNA 2'-phosphotransferase
-
RNA 2'-phosphotransferase
-
-
RNA 2'-phosphotransferase
-
-
RNA 2'-phosphotransferase
-
RNA 2'-phosphotransferase
-
RNA 2'-phosphotransferase
-
-
RNA 2'-phosphotransferase
-
RNA 2'-phosphotransferase
-
-
RNA 2'-phosphotransferase
-
-
RNA 2'-phosphotransferase
-
-
RNA 2'-phosphotransferase
-
-
RNA 2'-phosphotransferase
-
-
RNA 2'-phosphotransferase
-
-
RNA 2'-phosphotransferase
-
RNA 2'-phosphotransferase
-
-
Tpt1

-
-
Tpt1p

-
Mammalian genomes encode a single known functional homolog of yeast Tpt1p.
tRNA 2'-phosphotransferase

-
-
tRNA 2'-phosphotransferase
-
-
tRNA 2'-phosphotransferase
-
-
tRNA 2'-phosphotransferase
-
-
tRNA 2'-phosphotransferase
-
-
tRNA 2'-phosphotransferase
-
tRNA 2'-phosphotransferase
-
-
tRNA 2'-phosphotransferase
-
-
tRNA 2'-phosphotransferase
-
-
tRNA 2'-phosphotransferase
-
-
tRNA 2'-phosphotransferase
-
-
tRNA 2'-phosphotransferase
-
-
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
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18-mer 2' phosphorylated RNA
?
18-mer 2' phosphorylated RNA + NAD+
?
-
ACAAGACUGUAA-(2-phosphate)AUCUUG
-
-
?
2'-phospho-(ligated tRNA) + NAD+
mature tRNA + ADP-ribose 1'',2''-phosphate + nicotinamide + H+
-
-
-
-
?
2'-phospho-(ligated tRNA) + NAD+
mature tRNA + ADP-ribose 1'',2''-phosphate + nicotinamide + H2O
yeasts
-
enzyme catalyzes final step in tRNA splicing
-
-
?
2'-phospho-(ligated tRNA) + NAD+
mature tRNA + ADP-ribose1'',2''-phosphate + nicotinamide + H2O
-
last step in the pre-tRNA splicing process
-
-
ir
2'-phospho-[ligated tRNA]
?
2'-phospho-[ligated tRNA] + NAD+
mature tRNA + ADP ribose-1'',2''-phosphate + nicotinamide + H2O
2'-phospho-[ligated tRNA] + NAD+
mature tRNA + ADP-ribose 1",2"-phosphate + nicotinamide
3-mer 2' phosphorylated RNA
?
3-mer 2' phosphorylated RNA + NAD+
?
-
AU-(2-phosphate)U
-
-
?
8-mer 2' phosphorylated RNA
?
8-mer 2' phosphorylated RNA + NAD+
?
-
GUAA-(2-phosphate)AUCU
-
-
?
NAD+ + trinucleotide substrate
ADP-ribosylated RNA
-
requires ca. 0.0004 mM Tpt1 K69A/R71S, only 0.0000003 mM Tpt1p is required for similar or greater product formation
-
-
?
additional information
?
-
18-mer 2' phosphorylated RNA

?
-
-
-
-
?
18-mer 2' phosphorylated RNA
?
-
pACAAGACUCUAA-(2-phosphate)AUCUUG
-
-
?
2'-phospho-[ligated tRNA]

?
-
-
-
-
?
2'-phospho-[ligated tRNA]
?
-
-
-
-
?
2'-phospho-[ligated tRNA] + NAD+

mature tRNA + ADP ribose-1'',2''-phosphate + nicotinamide + H2O
-
-
-
-
?
2'-phospho-[ligated tRNA] + NAD+
mature tRNA + ADP ribose-1'',2''-phosphate + nicotinamide + H2O
-
-
-
-
?
2'-phospho-[ligated tRNA] + NAD+
mature tRNA + ADP ribose-1'',2''-phosphate + nicotinamide + H2O
-
-
-
-
?
2'-phospho-[ligated tRNA] + NAD+
mature tRNA + ADP ribose-1'',2''-phosphate + nicotinamide + H2O
-
production of a reaction intermediate, nicotinamide release occurs during intermediate formation, KptA can catalyze the conversion of the intermediate to both product and the original substrate
-
-
?
2'-phospho-[ligated tRNA] + NAD+
mature tRNA + ADP ribose-1'',2''-phosphate + nicotinamide + H2O
-
minimal substrate is a dinucleotide containing an internal 2' phosphate, 600fold more specific for 2'phosphates than for eihter 5'- or 3'-terminal phosphates, 20fold more specific for internal 2' phosphates than for terminal 2' 3', or 2'3'-cyclic phosphates, at least 50fold more specific for internal 2' phosphates than terminal 2' phosphates
-
-
?
2'-phospho-[ligated tRNA] + NAD+
mature tRNA + ADP ribose-1'',2''-phosphate + nicotinamide + H2O
-
-
-
-
?
2'-phospho-[ligated tRNA] + NAD+
mature tRNA + ADP ribose-1'',2''-phosphate + nicotinamide + H2O
-
mTpt1 can catalyze the conversion of the KptA-generated intermediate to both product and the original substrate
-
-
?
2'-phospho-[ligated tRNA] + NAD+
mature tRNA + ADP ribose-1'',2''-phosphate + nicotinamide + H2O
-
-
-
-
?
2'-phospho-[ligated tRNA] + NAD+
mature tRNA + ADP ribose-1'',2''-phosphate + nicotinamide + H2O
-
-
-
?
2'-phospho-[ligated tRNA] + NAD+
mature tRNA + ADP ribose-1'',2''-phosphate + nicotinamide + H2O
-
minimal substrate is a dinucleotide containing an internal 2'-phosphate
-
-
?
2'-phospho-[ligated tRNA] + NAD+
mature tRNA + ADP ribose-1'',2''-phosphate + nicotinamide + H2O
-
specifity for substrates bearing an internal 2'-phosphate
-
-
?
2'-phospho-[ligated tRNA] + NAD+
mature tRNA + ADP ribose-1'',2''-phosphate + nicotinamide + H2O
-
Tpt1 can catalyze the conversion of the KptA-generated intermediate to both product and the original substrate
-
-
?
2'-phospho-[ligated tRNA] + NAD+
mature tRNA + ADP ribose-1'',2''-phosphate + nicotinamide + H2O
-
tRNA_Leu_UAG, tRNA_SER, tRNA_LEU_CAA, tRNA_TYR, tRNA_LYS, tRNA_PRO1, tRNA_PRO2, tRNA_PHE, unidentified tRNA
-
-
?
2'-phospho-[ligated tRNA] + NAD+
mature tRNA + ADP ribose-1'',2''-phosphate + nicotinamide + H2O
-
-
-
-
?
2'-phospho-[ligated tRNA] + NAD+

mature tRNA + ADP-ribose 1",2"-phosphate + nicotinamide
-
-
-
?
2'-phospho-[ligated tRNA] + NAD+
mature tRNA + ADP-ribose 1",2"-phosphate + nicotinamide
-
-
-
?
2'-phospho-[ligated tRNA] + NAD+
mature tRNA + ADP-ribose 1",2"-phosphate + nicotinamide
C0R0E9
-
-
-
?
2'-phospho-[ligated tRNA] + NAD+
mature tRNA + ADP-ribose 1",2"-phosphate + nicotinamide
C0R0E9
-
-
-
?
2'-phospho-[ligated tRNA] + NAD+
mature tRNA + ADP-ribose 1",2"-phosphate + nicotinamide
-
-
-
?
2'-phospho-[ligated tRNA] + NAD+
mature tRNA + ADP-ribose 1",2"-phosphate + nicotinamide
-
-
-
?
2'-phospho-[ligated tRNA] + NAD+
mature tRNA + ADP-ribose 1",2"-phosphate + nicotinamide
-
-
-
?
2'-phospho-[ligated tRNA] + NAD+
mature tRNA + ADP-ribose 1",2"-phosphate + nicotinamide
-
-
-
?
2'-phospho-[ligated tRNA] + NAD+
mature tRNA + ADP-ribose 1",2"-phosphate + nicotinamide
-
-
-
?
2'-phospho-[ligated tRNA] + NAD+
mature tRNA + ADP-ribose 1",2"-phosphate + nicotinamide
-
-
-
?
2'-phospho-[ligated tRNA] + NAD+
mature tRNA + ADP-ribose 1",2"-phosphate + nicotinamide
-
-
-
?
2'-phospho-[ligated tRNA] + NAD+
mature tRNA + ADP-ribose 1",2"-phosphate + nicotinamide
-
-
-
?
2'-phospho-[ligated tRNA] + NAD+
mature tRNA + ADP-ribose 1",2"-phosphate + nicotinamide
-
-
-
?
2'-phospho-[ligated tRNA] + NAD+
mature tRNA + ADP-ribose 1",2"-phosphate + nicotinamide
-
-
-
?
2'-phospho-[ligated tRNA] + NAD+
mature tRNA + ADP-ribose 1",2"-phosphate + nicotinamide
-
-
-
?
2'-phospho-[ligated tRNA] + NAD+
mature tRNA + ADP-ribose 1",2"-phosphate + nicotinamide
-
-
-
?
2'-phospho-[ligated tRNA] + NAD+
mature tRNA + ADP-ribose 1",2"-phosphate + nicotinamide
-
-
-
?
2'-phospho-[ligated tRNA] + NAD+
mature tRNA + ADP-ribose 1",2"-phosphate + nicotinamide
-
-
-
?
2'-phospho-[ligated tRNA] + NAD+
mature tRNA + ADP-ribose 1",2"-phosphate + nicotinamide
-
-
-
-
?
2'-phospho-[ligated tRNA] + NAD+
mature tRNA + ADP-ribose 1",2"-phosphate + nicotinamide
-
-
-
?
2'-phospho-[ligated tRNA] + NAD+
mature tRNA + ADP-ribose 1",2"-phosphate + nicotinamide
-
-
-
?
3-mer 2' phosphorylated RNA

?
-
pApa-(2-phosphate)pA, pUpU-(2-phosphate)pU, pAAA-(2-phosphate)p and pAAA-(2-phosphate)pOCH3
-
-
?
3-mer 2' phosphorylated RNA
?
-
pApA-(2-phosphate)pA, pApU-(2-phosphate)pA, pUpU-(2-phosphate)pU, pApApA-(2-phosphate),pApApA-(2-phosphate)p, pApApA-(2-phosphate)1OCH3
-
-
?
8-mer 2' phosphorylated RNA

?
-
-
-
-
?
8-mer 2' phosphorylated RNA
?
-
pGUAA-(2-phosphate)AUCU
-
-
?
additional information

?
-
-
Comparison of incorporation of 3H-tyrosine and 35S-methionine/cysteine into total proteins confirms that wild-type and Trpt1-/-cells have similar incorporation rates of either amino acid.
-
-
-
additional information
?
-
yeasts
-
synthesis of 5'-Lv-2'-DMT-3'-phosphoramidite-G monomer containing an internal 2-phosphate for incorporation in synthetic RNA in order to construct a fluorescent oligoribonucleotide probe for a high throughput screening assay against yeast Tpt1p.
-
-
-
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
2'-phospho-(ligated tRNA) + NAD+
mature tRNA + ADP-ribose 1'',2''-phosphate + nicotinamide + H+
-
-
-
-
?
2'-phospho-(ligated tRNA) + NAD+
mature tRNA + ADP-ribose 1'',2''-phosphate + nicotinamide + H2O
yeasts
-
enzyme catalyzes final step in tRNA splicing
-
-
?
2'-phospho-(ligated tRNA) + NAD+
mature tRNA + ADP-ribose1'',2''-phosphate + nicotinamide + H2O
-
last step in the pre-tRNA splicing process
-
-
ir
2'-phospho-[ligated tRNA] + NAD+
mature tRNA + ADP-ribose 1",2"-phosphate + nicotinamide
additional information
?
-
-
Comparison of incorporation of 3H-tyrosine and 35S-methionine/cysteine into total proteins confirms that wild-type and Trpt1-/-cells have similar incorporation rates of either amino acid.
-
-
-
2'-phospho-[ligated tRNA] + NAD+

mature tRNA + ADP-ribose 1",2"-phosphate + nicotinamide
Q8U9Z2
-
-
-
?
2'-phospho-[ligated tRNA] + NAD+
mature tRNA + ADP-ribose 1",2"-phosphate + nicotinamide
Q8U9Z2
-
-
-
?
2'-phospho-[ligated tRNA] + NAD+
mature tRNA + ADP-ribose 1",2"-phosphate + nicotinamide
C0R0E9
-
-
-
?
2'-phospho-[ligated tRNA] + NAD+
mature tRNA + ADP-ribose 1",2"-phosphate + nicotinamide
C0R0E9
-
-
-
?
2'-phospho-[ligated tRNA] + NAD+
mature tRNA + ADP-ribose 1",2"-phosphate + nicotinamide
C7QAQ2
-
-
-
?
2'-phospho-[ligated tRNA] + NAD+
mature tRNA + ADP-ribose 1",2"-phosphate + nicotinamide
C1CZ01
-
-
-
?
2'-phospho-[ligated tRNA] + NAD+
mature tRNA + ADP-ribose 1",2"-phosphate + nicotinamide
C1CZ01
-
-
-
?
2'-phospho-[ligated tRNA] + NAD+
mature tRNA + ADP-ribose 1",2"-phosphate + nicotinamide
H8GTD5
-
-
-
?
2'-phospho-[ligated tRNA] + NAD+
mature tRNA + ADP-ribose 1",2"-phosphate + nicotinamide
H8GTD5
-
-
-
?
2'-phospho-[ligated tRNA] + NAD+
mature tRNA + ADP-ribose 1",2"-phosphate + nicotinamide
Q9RRR1
-
-
-
?
2'-phospho-[ligated tRNA] + NAD+
mature tRNA + ADP-ribose 1",2"-phosphate + nicotinamide
Q9RRR1
-
-
-
?
2'-phospho-[ligated tRNA] + NAD+
mature tRNA + ADP-ribose 1",2"-phosphate + nicotinamide
A9C0S5
-
-
-
?
2'-phospho-[ligated tRNA] + NAD+
mature tRNA + ADP-ribose 1",2"-phosphate + nicotinamide
A9C0S5
-
-
-
?
2'-phospho-[ligated tRNA] + NAD+
mature tRNA + ADP-ribose 1",2"-phosphate + nicotinamide
A5FLZ4
-
-
-
?
2'-phospho-[ligated tRNA] + NAD+
mature tRNA + ADP-ribose 1",2"-phosphate + nicotinamide
Q8R5N7
-
-
-
?
2'-phospho-[ligated tRNA] + NAD+
mature tRNA + ADP-ribose 1",2"-phosphate + nicotinamide
Q8R5N7
-
-
-
?
2'-phospho-[ligated tRNA] + NAD+
mature tRNA + ADP-ribose 1",2"-phosphate + nicotinamide
A9B356
-
-
-
?
2'-phospho-[ligated tRNA] + NAD+
mature tRNA + ADP-ribose 1",2"-phosphate + nicotinamide
A9B356
-
-
-
?
2'-phospho-[ligated tRNA] + NAD+
mature tRNA + ADP-ribose 1",2"-phosphate + nicotinamide
-
-
-
-
?
2'-phospho-[ligated tRNA] + NAD+
mature tRNA + ADP-ribose 1",2"-phosphate + nicotinamide
D1APE3
-
-
-
?
2'-phospho-[ligated tRNA] + NAD+
mature tRNA + ADP-ribose 1",2"-phosphate + nicotinamide
D1APE3
-
-
-
?
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
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0.0000017 - 0.00015
18-mer 2' phosphorylated RNA
-
0.00000035
2'-phospho-[ligated tRNA]
-
-
0.00015 - 0.04
3-mer 2' phosphorylated RNA
-
0.00002 - 0.00025
8-mer 2' phosphorylated RNA
-
0.000004
[ligated tRNA]
-
-
-
0.0000017
18-mer 2' phosphorylated RNA

-
pACAAGACUCUAA-(2-phosphate)AUCUUG, 30°C
-
0.00015
18-mer 2' phosphorylated RNA
-
37°C
-
0.00015
3-mer 2' phosphorylated RNA

-
pApA-(2-phosphate)pA, 30°C
-
0.000325
3-mer 2' phosphorylated RNA
-
pUpU-(2-phosphate)pUs, 30°C
-
0.00048
3-mer 2' phosphorylated RNA
-
pApU-(2-phosphate)pA, 30°C
-
0.00065
3-mer 2' phosphorylated RNA
-
pApApA-(2-phosphate)p, 30°C
-
0.0007
3-mer 2' phosphorylated RNA
-
pApA-(2-phosphate)pA, 37°C
-
0.0013
3-mer 2' phosphorylated RNA
-
pAAA-(2-phosphate)p, 37°C
-
0.0018
3-mer 2' phosphorylated RNA
-
pUpU-(2-phosphate)pU, 37°C
-
0.003
3-mer 2' phosphorylated RNA
-
pApApA-(2-phosphate)pOCH3, 30°C
-
0.0038
3-mer 2' phosphorylated RNA
-
pAAA-(2-phosphate)pOCH3, 37°C
-
0.04
3-mer 2' phosphorylated RNA
-
pApApA-(2-phosphate), 30°C
-
0.00002
8-mer 2' phosphorylated RNA

-
pGUAA-(2-phosphate)AUCU, 30°C
-
0.00025
8-mer 2' phosphorylated RNA
-
37°C
-
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
0.04
18-mer 2' phosphorylated RNA
-
pACAAGACUCUAA-(2-phosphate)AUCUUG, 30°C
-
0.0016
2'-phospho-[ligated tRNA]
-
-
0.0057 - 6.08
3-mer 2' phosphorylated RNA
-
0.317
8-mer 2' phosphorylated RNA
-
pGUAA-(2-phosphate)AUCU, 30°C
-
additional information
18-mer 2' phosphorylated RNA
-
0.0057
3-mer 2' phosphorylated RNA

-
pApApA-(2-phosphate), 30°C
-
0.147
3-mer 2' phosphorylated RNA
-
pUpU-(2-phosphate)pU, 30°C
-
0.15
3-mer 2' phosphorylated RNA
-
pApApA-(2-phosphate)p, 30°C
-
0.45
3-mer 2' phosphorylated RNA
-
pApU-(2-phosphate)pA, 30°C
-
0.73
3-mer 2' phosphorylated RNA
-
pApA-(2-phosphate)pA, 30°C
-
1.173
3-mer 2' phosphorylated RNA
-
pApApA-(2-phosphate)pOCH3, 30°C
-
6.08
3-mer 2' phosphorylated RNA
-
pApApA-(2-phosphate)pOCH3, 30°C
-
additional information
18-mer 2' phosphorylated RNA

-
37°C, vmax = 99 fmol/min/ng
-
additional information
3-mer 2' phosphorylated RNA
-
pAAA-(2-phosphate)p, 37°C, vmax = 1.4 fmol/min/ng; pAAA-(2-phosphate)pOCH3, 37°C, vmax = 45 fmol/min/ng; pApA-(2-phosphate)pA, 37°C, vmax = 280 fmol/min/ng; pUpU-(2-phosphate)pU, 37°C, vmax = 1030 fmol/min/ng
-
additional information
8-mer 2' phosphorylated RNA
-
37°C, vmax = 170 fmol/min/ng
-
additional information
[ligated tRNA]
-
vmax = 0.26 fmol/min/ng
-
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
expression from Saccharomyces cerevisiae vectors
-
expression in Escherichia coli
expression in Escherichia coli as a His-6 fusion
-
expression in Escherichia coli as a His-6 fusion, expression in Saccharomyces cerevisiae tpt1 mutant
expression in Escherichia coli B834(DE3)
-
expression in Escherichia coli BL21(DE3)
-
expression in Escherichia coli strain RZ510
expression in Saccharomyces cerevisiae
-
expression in Saccharomyces cerevisiae tpt1 mutant strain Y2, the human TRPT1 gene could complement this mutation
expression in Saccharomyces cerevisiae tpt1 mutant strain Y2, the wild-type yeast TPT1 could complement this mutation
-
expression in Saccharomyces cerevisiae tpt1 mutants
Fusion of the coding region of the splicing enzyme in-frame to the amino-terminus of the green fluorescent protein (GFP). Full-length 2'-phosphotransferase cDNA (289 aa) and a short version of the same polypeptide starting with the second methionine at position 33 are fused to the coding region of GFP. Additionally, the N-terminal sequence of 97 amino acids derived from the 2'-phosphotransferase is directly fused to GFP.
-
gene kptA, encoded in an operon-like cluster with genes encoding RNA polymerase, RNA ligase, and the Hen1/Pnkp RNA repairsystem reported to repair ribotoxin-degraded tRNAs in vitro
gene kptA, genomic organization, comparisons
gene kptA, genomic organization, comparisons, the gene kpta neighborhood includes the genes coding for: (i) the enzyme Nam phosphoribo-syltransferase (NadV), involved in the recycling to NAD of Nam, thepyridine product of the KptA-catalyzed reaction, (ii) the orthologue of the yeast ADPR-1-P phosphatase, implicated in the conversionto ADPR of the adenylylic product, (iii) the enzyme ADPR diphosphatase (ADPRP), responsible for ADPR hydrolysis to AMPand R5P, and (iv) the enzyme PRPP synthetase, which catalyzes PRPP formation from ATP and ribose 5-phosphate
overexpression in Escherichia coli BL21(DE3)
-
The murine Trpt1 gene is targeted in E14 ES cells with a positive/negative selection vector. Trpt1 gene is disrupted by homologous recombination in ES cells, deleting exons 3, 4, and part of exon 5 that encode amino acids 48-173. Clones from two individually selected heterozygous ES cell lines are injected into blastocysts and the resultant founder chimeras bred to obtain two lines of Trpt1+/- and Trpt1-/-mice that are both viable and healthy and are normally fertile. Cell culture: Trpt1+/+ and Trpt1-/-cell lines are obtained by serial passage of SV40-T-antigen transfected mouse embryonic fibroblasts.
-
expression in Escherichia coli

-
expression in Escherichia coli
-
expression in Escherichia coli
-
expression in Escherichia coli
-
expression in Escherichia coli as a His-6 fusion, expression in Saccharomyces cerevisiae tpt1 mutant

-
expression in Escherichia coli as a His-6 fusion, expression in Saccharomyces cerevisiae tpt1 mutant
-
expression in Saccharomyces cerevisiae tpt1 mutants

-
expression in Saccharomyces cerevisiae tpt1 mutants
-
gene kptA, genomic organization, comparisons

gene kptA, genomic organization, comparisons
C0R0E9
gene kptA, genomic organization, comparisons
gene kptA, genomic organization, comparisons
gene kptA, genomic organization, comparisons
gene kptA, genomic organization, comparisons
gene kptA, genomic organization, comparisons
gene kptA, genomic organization, comparisons
gene kptA, genomic organization, comparisons
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
C85R
-
no accumulation of the reaction intermediate
D34A
-
no accumulation of the reaction intermediate
F72A
-
temperature sensitive
H117A-T119A
-
lethal in vivo
H140A
-
no accumulation of the reaction intermediate
H140A/H142A
-
no accumulation of the reaction intermediate
H140A/V141A/H142A
-
no accumulation of the reaction intermediate
I167L/D168K
-
no accumulation of the reaction intermediate
K16A
-
colony size indistinguishable from that of wild-type TPT1 strain
K69A
-
colony size indistinguishable from that of wild-type TPT1 strain
K69A/R71S
-
this mutant produces a substantial amount of the reaction intermediate
M136G
-
no accumulation of the reaction intermediate
N185A
-
no accumulation of the reaction intermediate
Q88A/H90A/S91A
-
no accumulation of the reaction intermediate
Q88Y
-
no accumulation of the reaction intermediate
R138K
-
colony size indistinguishable from that of wild-type TPT1 strain
R158A
-
colony size indistinguishable from that of wild-type TPT1 strain
R158H
-
no accumulation of the reaction intermediate
R23A/H24A
-
no accumulation of the reaction intermediate
R71Q
-
grows slower than wild-type, temperature sensitive
S155A
-
no accumulation of the reaction intermediate
S15A/K16A
-
no accumulation of the reaction intermediate
S183A
-
no accumulation of the reaction intermediate
T119A
-
colony size indistinguishable from that of wild-type TPT1 strain
Y38A
-
colony size indistinguishable from that of wild-type TPT1 strain
H117A

-
colony size indistinguishable from that of wild-type TPT1 strain
H117A
-
no accumulation of the reaction intermediate
H142A

-
colony size indistinguishable from that of wild-type TPT1 strain
H142A
-
no accumulation of the reaction intermediate
H90A

-
colony size indistinguishable from that of wild-type TPT1 strain
H90A
-
no accumulation of the reaction intermediate
R138A

-
lethal in vivo
R138A
-
no accumulation of the reaction intermediate
S91A

-
colony size indistinguishable from that of wild-type TPT1 strain
S91A
-
no accumulation of the reaction intermediate
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Culver, G.M.; McCraith, S.M.; Consaul, S.A.; Stanford, D.R.; Phizicky, E.M.
A 2'-phosphotransferase implicated in tRNA splicing is essential in Saccharomyces cerevisiae
J. Biol. Chem.
272
13203-13210
1997
Escherichia coli, Homo sapiens, Mus musculus, Saccharomyces cerevisiae (Q12272), Saccharomyces cerevisiae, Schizosaccharomyces pombe
brenda
Steiger, M.A.; Kierzek, R.; Turner, D.H.; Phizicky, E.M.
Substrate recognition by a yeast 2'-phosphotransferase involved in tRNA splicing and by its Escherichia coli homolog
Biochemistry
40
14098-14105
2001
Escherichia coli, Saccharomyces cerevisiae
brenda
Hu, Q.D.; Lu, H.; Huo, K.; Ying, K.; Li, J.; Xie, Y.; Mao, Y.; Li, Y.Y.
A human homolog of the yeast gene encoding tRNA 2'-phosphotransferase: cloning, characterization and complementation analysis
Cell. Mol. Life Sci.
60
1725-1732
2003
Arabidopsis thaliana, Archaeoglobus fulgidus, Clostridium perfringens, Escherichia coli, Fusobacterium nucleatum, Homo sapiens, Homo sapiens (Q86TN4), Methanopyrus kandleri, Mus musculus, Rattus norvegicus, Saccharomyces cerevisiae, Schizosaccharomyces pombe, Streptomyces coelicolor
brenda
Mc Craith, S.M.; Phizicky, E.M.
An enzyme from Saccharomyces cerevisiae uses NAD+ to transfer the splice junction 2'-phosphate from ligated tRNA to an acceptor molecule
J. Biol. Chem.
266
11986-11992
1991
Saccharomyces cerevisiae
brenda
Zillman, M.; Gorovsky, M.A.; Phizicky, E.M.
HeLa cells contain a 2'-phosphate-specific phosphotransferase similar to a yeast enzyme implicated in tRNA splicing
J. Biol. Chem.
267
10289-10294
1992
Homo sapiens, Saccharomyces cerevisiae
brenda
Spinelli, S.L.; Kierzek, R.; Turner, D.H.; Phizicky, E.M.
Transient ADP-ribosylation of a 2'-phosphate implicated in its removal from ligated tRNA during splicing in yeast
J. Biol. Chem.
274
2637-2644
1999
Escherichia coli, Mus musculus, Saccharomyces cerevisiae
brenda
Kato-Murayama, M.; Bessho, Y.; Shirouzu, M.; Yokoyama, S.
Crystal structure of the RNA 2'-phosphotransferase from Aeropyrum pernix K1
J. Mol. Biol.
348
295-305
2005
Aeropyrum pernix, Arabidopsis thaliana, Archaeoglobus fulgidus, Escherichia coli, Homo sapiens, Methanosarcina mazei, Pseudomonas aeruginosa, Pyrococcus horikoshii, Saccharomyces cerevisiae
brenda
Kierzek, R.; Steiger, M.A.; Spinelli, S.L.; Turner, D.H.; Phizicky, E.M.
The chemical synthesis of oligoribonucleotides with selectively placed 2'-O-phosphates
Nucleosides Nucleotides Nucleic Acids
19
917-933
2000
Saccharomyces cerevisiae
brenda
Spinelli, S.L.; Malik, H.S.; Consaul, S.A.; Phizicky, E.M.
A functional homolog of a yeast tRNA splicing enzyme is conserved in higher eukaryotes and in Escherichia coli
Proc. Natl. Acad. Sci. USA
95
14136-14141
1998
Arabidopsis thaliana, Archaeoglobus fulgidus, Candida albicans, Escherichia coli, Mus musculus, no activity in Bacillus subtilis, no activity in Haemophilus influenzae, no activity in Helicobacter pylori, no activity in Mycoplasma genitalium, Pseudomonas aeruginosa, Pyrococcus horikoshii, Saccharomyces cerevisiae, Schizosaccharomyces pombe
brenda
Sawaya, R.; Schwer, B.; Shuman, S.
Structure-function analysis of the yeast NAD+-dependent tRNA 2'-phosphotransferase Tpt1
RNA
11
107-113
2005
Aeropyrum pernix, Archaeoglobus fulgidus, Clostridium perfringens, Drosophila melanogaster, Escherichia coli, Homo sapiens, Leishmania major, Nostoc punctiforme, Pyrococcus horikoshii, Saccharomyces cerevisiae, Schizosaccharomyces pombe, Trypanosoma cruzi
brenda
Steiger, M.A.; Jackman, J.E.; Phizicky, E.M.
Analysis of 2'-phosphotransferase (Tpt1p) from Saccharomyces cerevisiae: evidence for a conserved two-step reaction mechanism
RNA
11
99-106
2005
Saccharomyces cerevisiae
brenda
Spinelli, S.L.; Consaul, S.A.; Phizicky, E.M.
A conditional lethal yeast phosphotransferase (tpt1) mutant accumulates tRNAs with a 2'-phosphate and an undermodified base at the splice junction
RNA
3
1388-1400
1997
Escherichia coli, Saccharomyces cerevisiae
brenda
Englert, M.; Latz, A.; Becker, D.; Gimple, O.; Beier, H.; Akama, K.
Plant pre-tRNA splicing enzymes are targeted to multiple cellular compartments
Biochimie
89
1351-1365
2007
Arabidopsis thaliana
brenda
Lackey, J.G.; Ron, D.; Damha, M.J.; Harding, H.P.
Toward the discovery of new antifungal agents: the design and validation of a novel 2P-RNA probe and high throughput screening assay against 2-phosphotransferase Tpt1p
Nucleic Acids Symp. Ser.
52
475-476
2008
yeasts
brenda
Harding, H.P.; Lackey, J.G.; Hsu, H.C.; Zhang, Y.; Deng, J.; Xu, R.M.; Damha, M.J.; Ron, D.
An intact unfolded protein response in Trpt1 knockout mice reveals phylogenic divergence in pathways for RNA ligation
RNA
14
225-232
2008
Mus musculus
brenda
Sorci, L.; Ruggieri, S.; Raffaelli, N.
NAD homeostasis in the bacterial response to DNA/RNA damage
DNA Repair
23
17-26
2014
Agrobacterium tumefaciens (Q8U9Z2), Agrobacterium tumefaciens C58 / ATCC 33970 (Q8U9Z2), Brachyspira hyodysenteriae (C0R0E9), Brachyspira hyodysenteriae ATCC 49526 (C0R0E9), Catenulispora acidiphila (C7QAQ2), Deinococcus deserti (C1CZ01), Deinococcus deserti DSM 17065 (C1CZ01), Deinococcus gobiensis (H8GTD5), Deinococcus gobiensis DSM 21396 (H8GTD5), Deinococcus radiodurans (Q9RRR1), Deinococcus radiodurans ATCC 13939 (Q9RRR1), Delftia acidovorans (A9C0S5), Delftia acidovorans DSM 14801 (A9C0S5), Flavobacterium johnsoniae (A5FLZ4), Fusobacterium nucleatum subsp. nucleatum (Q8R5N7), Fusobacterium nucleatum subsp. nucleatum ATCC 25586 / JCM14847 (Q8R5N7), Herpetosiphon aurantiacus (A9B356), Herpetosiphon aurantiacus DSM 785 (A9B356), Saccharomyces cerevisiae, Sebaldella termitidis (D1APE3), Sebaldella termitidis ATCC 33386 (D1APE3)
brenda