EC Number |
Application |
Reference |
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5.1.3.3 | analysis |
conductometric biosensor for sucrose determination using a membrane containing enzymes invertase, mutarotase, and glucose oxidase as a sensitive element immobilized on the conductometric interdigitated planar electrodes. The biosensor demonstrates high selectivity, operational stability and reproducibility |
693972 |
5.1.3.3 | analysis |
kinetic assay method for aldose 1-epimerase based upon fast in situ generation of alpha-D-glucose employing hydrolysis of sucrose by beta-fructofuranosidase and a subsequent reporter reaction involving the aerobic oxidation of beta-D-glucose via glucose o |
2371 |
5.1.3.3 | analysis |
rapid determination of glucose by aldose 1-epimerase |
2374 |
5.1.3.3 | synthesis |
improvement of a bacterial L-arabinose utilization pathway consisting of L-arabinose isomerase from Bacillus licheniformis and L-ribulokinase and L-ribulose-5-phosphate 4-epimerase from Escherichia coli after expression of the corresponding genes in Saccharomyces cerevisiae. After adaptation of codon usage, yeast transformants show strongly improved L-arabinose conversion rates. The ethanol production rate from L-arabinose can be increased more than 2.5fold from 0.014 g ethanol per h and g dry weight to 0.036 g ethanol per h and g dry weight and the ethanol yield can be increased from 0.24 g ethanol per g consumed L-arabinose to 0.39 g ethanol per g consumed L-arabinose |
684574 |