EC Number   |
Application |
Reference |
|---|
    3.5.3.3 | analysis |
biomatrix fabricated to investigate immobilization of creatine amidinohydrolase |
-, 698318 |
| 3.5.3.3 | analysis |
construction of a biosensor by co-immobilization of creatininase, creatinase, and sarcosine oxidase onto iron oxide nanoparticles/chitosangraft-polyaniline, Fe3O4-NPs/CHIT-g-PANI, composite film electrodeposited on surface of Pt electrode through glutaraldehyde coupling. The creatinine biosensor uses enzymes/Fe3O4-NPs/CHIT-g-PANI/Pt electrode as working electrode, Ag/AgCl as reference electrode and Pt wire as auxiliary electrode. The biosensor exhibits an optimum response within 2 s at pH 7.5 and 30°C, when polarized at 0.4 V versus Ag/AgCl. The electrocatalytic response shows a linear dependence on creatinine concentration ranging from 1 to 800 M. The sensitivity of the biosensor is 3.9 microA per microM and cm2, with a detection limit of 1 microM. The biosensor shows only 10% loss in its initial response after 120 uses over 200 days, when stored at 4°C. The biosensor measures creatinine in the serum of apparently healthy persons |
719377 |
| 3.5.3.3 | diagnostics |
creatininase is used as clinical enzyme to measure creatinine. Spores co-expressing creatininase and creatinase mediate a two-step reaction from creatinine to urea (and sarcosine). An advantage of spore-encapsulated enzymes is that immobilized and stress-tolerant enzymes can be produced without purification |
753055 |
| 3.5.3.3 | diagnostics |
important medical enzyme, used for clinical diagnosis of renal function because of its high substrate specificity |
752873 |
| 3.5.3.3 | diagnostics |
recombinant production of creatinase for using in clinical measurement of serum or urine creatinine |
-, 752626 |
| 3.5.3.3 | diagnostics |
yeast spore-based encapsulation technique is applied to the diagnostic enzyme creatinase. A secretory form of Pseudomonas putida creatinase can be entrapped in the spore wall, and such spores are used as creatinase capsules. The activity of the encapsulated creatinase is largely improved by mild spore wall defective mutations, such as DIT1 or OSW2 deletions. The encapsulated and freeze-dried creatinase is produced without preparing the purified enzyme, and it exhibits resistance to environmental stresses, such as high temperature and SDS treatments. Thus, yeast spores can be applied to establish quick and easy clinical diagnostic methods |
752875 |
| 3.5.3.3 | medicine |
creatinase may play a role in the disease process caused by Actinobacillus |
663473 |
| 3.5.3.3 | medicine |
creatinine determination in biological fluids |
-, 663469 |
