Application | Comment | Organism |
---|---|---|
diagnostics | yeast spore-based encapsulation technique is applied to the diagnostic enzyme creatinase. A secretory form of Pseudomonas putida creatinase can be entrapped in the spore wall, and such spores are used as creatinase capsules. The activity of the encapsulated creatinase is largely improved by mild spore wall defective mutations, such as DIT1 or OSW2 deletions. The encapsulated and freeze-dried creatinase is produced without preparing the purified enzyme, and it exhibits resistance to environmental stresses, such as high temperature and SDS treatments. Thus, yeast spores can be applied to establish quick and easy clinical diagnostic methods | Pseudomonas putida |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
7 | - |
Creatine | pH 7.7, 50°C, creatinase in yeast spores with DIT1 deletion | Pseudomonas putida | |
7 | - |
Creatine | pH 7.7, 50°C, soluble enzyme | Pseudomonas putida | |
7.8 | - |
Creatine | pH 7.7, 50°C, creatinase in yeast spores with OSW2 deletion | Pseudomonas putida | |
10.9 | - |
Creatine | pH 7.7, 50°C, creatinase in wild-type yeast spores | Pseudomonas putida |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
creatine + H2O | Pseudomonas putida | - |
sarcosine + urea | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pseudomonas putida | P38488 | - |
- |
Source Tissue | Comment | Organism | Textmining |
---|
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
creatine + H2O | - |
Pseudomonas putida | sarcosine + urea | - |
? |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
80 | 90 | the activities of creatinase in wild-type and osw2D spores are more stable than those of free creatinase at higher temperatures. Such thermostability is not observed for creatinase in dit1D spores | Pseudomonas putida |