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EC Number	Application	Commentary	Reference
3.4.21.B50	medicine	the purified recombinant DegQVh is a protective immunogen that could confer protection upon fish against infection by Vibrio harveyi. In order to improve the efficiency of DegQVh as a vaccine, a genetic construct in the form of the plasmid pAQ1 is built, in which the DNA encoding the processed DegQVh protein is fused with the DNA encoding the secretion region of AgaV, an extracellular beta-agarase. The Escherichia coli strain harboring pAQ1 can express and secrete the chimeric DegQVh protein into the culture supernatant. Vaccination of fish with viable Escherichia coli expressing chimeric degQVh significantly enhanced the survival of fish against Vibrio harveyi challenge, which is possibly due to the relatively prolonged exposure of the immune system to the recombinant antigen produced constitutively	690548

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Release 2023.1 (January 2023)