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Literature summary for 3.4.21.B50 extracted from
- Sun, K.; Cheng, S.; Sun, L.: Characterization of DegQVh, a serine protease and a protective immunogen from a pathogenic Vibrio harveyi strain (2008), Appl. Environ. Microbiol., 74, 6254-6262.
Application
| Application | Comment | Organism |
|---|---|---|
| medicine | the purified recombinant DegQVh is a protective immunogen that could confer protection upon fish against infection by Vibrio harveyi. In order to improve the efficiency of DegQVh as a vaccine, a genetic construct in the form of the plasmid pAQ1 is built, in which the DNA encoding the processed DegQVh protein is fused with the DNA encoding the secretion region of AgaV, an extracellular beta-agarase. The Escherichia coli strain harboring pAQ1 can express and secrete the chimeric DegQVh protein into the culture supernatant. Vaccination of fish with viable Escherichia coli expressing chimeric degQVh significantly enhanced the survival of fish against Vibrio harveyi challenge, which is possibly due to the relatively prolonged exposure of the immune system to the recombinant antigen produced constitutively | Vibrio harveyi |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression in Escherichia coli | Vibrio harveyi |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| D113A | mutant exhibits 97.5% reduction in activity | Vibrio harveyi |
| H83A | mutant exhibits 93.4% reduction in activity | Vibrio harveyi |
| S188A | mutant exhibits 91.8% reduction in activity | Vibrio harveyi |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| phenylmethanesulfonyl fluoride | - |
Vibrio harveyi |  |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mn2+ | 10 mM, 42% increase in activity | Vibrio harveyi | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Vibrio harveyi | B6CND6 | strain T4, a pathogenic strain isolated from diseased fish | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant enzyme | Vibrio harveyi |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| casein + H2O | endoprotease, active serine protease whose activity requires the integrity of the catalytic site and the PDZ domains | Vibrio harveyi | hydrolyzed casein | - |
? | |
| additional information | recombinant DegQVh can not degrade bovine serum albumin and collagen. The purified recombinant DegQVh is a protective immunogen that could confer protection upon fish against infection by Vibrio harveyi | Vibrio harveyi | ? | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| DegQ | - |
Vibrio harveyi |
| DegQVh | - |
Vibrio harveyi |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 50 | - |
recombinant enzyme | Vibrio harveyi |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 40 | 55 | exhibits more than 70% of the maximum activity over the temperature range of 40 to 55°C | Vibrio harveyi |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 50 | - |
1 h, enzyme retains 81% activity | Vibrio harveyi |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8 | - |
recombinant enzyme | Vibrio harveyi |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 5 | 9 | exhibits more than 70% of the maximum activity over the pH range of 5 to 9 | Vibrio harveyi |
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