EC Number   |
Application |
Reference |
|---|
    2.7.2.3 | analysis |
computational docking method for analysis of the docking of both aromatic and aliphatic bisphosphonates to the Trypanosoma brucei PGK |
681295 |
| 2.7.2.3 | analysis |
development of a sensitive sandwich ELISA using an immuno-affinity-purified chicken polyclonal antibody for capturing PGK1 and an immuno-affinity-purified rabbit polyclonal antibody for detecting it. The sandwich ELISA can be used to quantify PGK1 levels in conditioned media of cell cultures for in vitro PGK1 export studies and in human serum for potential use ascreen for cancer |
688148 |
| 2.7.2.3 | analysis |
first quantitative analysis of the binding of alkyl bisphosphonates to yeast PGK, with activities being predicted within, on average, a factor of 4 over a 2500x overall range in activity |
681295 |
| 2.7.2.3 | analysis |
first quantitative analysis of the inhibition of human phosphoglycerate kinase by aromatic bisphosphonates by 3D-QSAR (CoMFA, CoMSIA) and pharmacophore modeling methods |
681295 |
| 2.7.2.3 | analysis |
in situ PCR is useful for detecting PGK-1 gene in paraffin-embedded sections under optimized conditions of both PCR cycle number and proteinase K concentration |
701562 |
| 2.7.2.3 | analysis |
PGK1 might be a potential protein biomarker of intracellular oxidative status |
685682 |
| 2.7.2.3 | biofuel production |
proteome analysis as well as enzyme assays performed in cell-free extracts demonstrates that glycerol is degraded via glyceraldehyde-3-phosphate, which is further metabolized through the lower part of glycolysis leading to formation of mainly ethanol and hydrogen. Fermentation of glycerol to ethanol and hydrogen by this bacterium represents a remarkable option to add value to the biodiesel industries by utilization of surplus glycerol |
748573 |
| 2.7.2.3 | medicine |
enzyme of parasite is target for vaccine and drug development, enzyme elicits antibodies and can therefore be utilized as immunoreagent in serodiagnostics for clonorchiasis |
642297 |
| 2.7.2.3 | medicine |
inhibition of the enzyme can provide a method of treatment for cardiovascular and respiratory disorders, construction of possible specific fluoro-phosphonate inhibitors |
642283 |
| 2.7.2.3 | medicine |
overexpression or down-regulation of PGK affects the cytotoxicity of beta-L-dioxolane-cytidine, which is under clinical trials for treating cancer, but not that of beta-D-2',2'-difluorodeoxycytidine under normoxic conditions, overexpression of PGK does not further increase the cytotoxicity of beta-L-dioxolane-cytidine under hypoxic conditions |
682025 |
