EC Number   |
Application |
Reference |
|---|
    2.4.1.20 | analysis |
quantitative determination of cellobiose in presence of glucose or glucose-1-phosphate |
637867 |
| 2.4.1.20 | synthesis |
ability of the phosphorylase to utilize alpha-D -glucose 1-fluoride as alternate glucosyl donor in place of alpha-D-glucose 1-phosphate for the synthesis of alpha-1,4-glucosides under thermodynamic control in close to 100% yield |
659742 |
| 2.4.1.20 | synthesis |
enzymatic cellobiose synthesis from starch using two phosphorylases: glucan phosphorylase, from Klebsiella pneumoniae, converts glucose residues in the starch into glucose-1-phosphate, removal of phosphate, and glucose-1-phosphate is incubated with alpha-D-glucose and is converted into cellobiose by recombinant cellobiose phosphorylase, removal of phosphate, 60% cellobiose yield from glucose 1-phosphate and, at least, 23.7% cellobiose yield from starch, method optimization, overview |
705837 |
| 2.4.1.20 | synthesis |
expression of enzyme in Escherichia coli and study on the role of molecular chaperones and growth temperature on the solubilization of enzyme overexpressed in Escherichia coli. The growth of host at low temperature enhances enzyme in soluble fraction. Similarly, induction of target gene at low level of IPTG also yields more enzyme in the soluble fraction. Coexpression of the target gene with molecular chaperones GroESL and KODH does not enhance the solubilization under in vivo conditions |
719140 |
| 2.4.1.20 | synthesis |
the cellobiose phosphorylases in the cell extract is used to synthesize radiolabeled cellodextrins with a degree of polymerization (DP=2-6) from non-radioactive glucose-1-phosphate and radioactive glucose. For cellobiose synthesis, the reaction is carried out at 60°C for 30 min with crude enzyme. After an impluse feed of radiolabeled cellobiose to a Clostridium thermocellum culture, the intracellular sugar levels are measured: the largest amount of radioactivity is cellobiose with lesser amounts of glucose, cellotriose, and cellotetraose, and an average degree of polymerization of intracellular cellodextrins is ca. 2 |
671515 |
| 2.4.1.20 | synthesis |
the enzyme is useful for production of D-glucose 1-phosphate, an expensive substrate for other enzymatic syntheses |
692802 |
| 2.4.1.20 | synthesis |
the enzyme is useful for synthesis of alkyl beta-glucosides, overview |
-, 702732 |
