EC Number |
Application |
Reference |
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1.7.1.16 | analysis |
fluorescence imaging of nitroreductase in zebrafish in vivo using a newnear-infrared fluorescence off-on probe. The probe is prepared by connecting 4-nitrobenzene as a quenching and recognizing moiety to a stable hemicyanine skeleton. The fluorescence off-on response of the probe is based on the enzyme-catalyzed reduction of the 4-nitrobenzene moiety, followed by the 1,6-rearrangement-elimination and the fluorophore release. The probe exhibits good analytical performance such as near-infrared fluorescence emission over 700 nm as well as high selectivity and sensitivity, with a detection limit of 14 ng/ml. The probe has been applied to visualize the distribution of nitroreductase in living zebrafish in vivo |
740192 |
1.7.1.16 | analysis |
immobilization of enzyme on polyethyleneimine-mediated silica formation, enzyme activity is significantly more stable than NbzA in solution. A microfluidic microreactor is suitable for continuous operation using nitrobenzene, CB1954, and the proantibiotic nitrofurazone. The flow-through system provides a rapid and reproducible screening method for determining the NbzA-catalyzed activation of prodrugs and proantibiotics |
-, 740133 |
1.7.1.16 | degradation |
the strain can use nitrobenzene as the sole carbon and nitrogen source for growth, and completely degrade 300 mg nitrobenzene per litre within 14 h, at 20-35°C and pH 7.0-9.0. Strain 1 can also degrade aniline and phenol |
-, 740325 |