EC Number   |
Application |
Reference |
|---|
   2.4.1.4 | industry |
the beta-carotene embedded amylose microparticles are prepared in one-step by utilizing the unique catalytic activity of amylosucrase from Deinococcus geothermalis, which synthesizes linear amylose chains using sucrose as the sole substrate. Synthesized amylose chains self-assembled with b-carotene to form well-defined spherical microparticles with an encapsulation yield of 65%. The synthetic method enables microparticle formation and beta-carotene encapsulation in one-step using amylosucrase and sucrose as the sole substrates, which indicates that the devised process may be cost-effective and suitable for industrial applications |
-, 756275 |
| 2.4.1.4 | industry |
the enzyme has great industrial potential owing to its multifunctional activities, including transglucosylation, polymerization, and isomerization |
-, 756626 |
| 2.4.1.4 | synthesis |
amylosucrase has great potential in the biotechnology and food industries, due to its multifunctional enzyme activities. It can synthesize alpha-1,4-glucans, like amylose, from sucrose as a sole substrate. It can also utilize various other molecules as acceptors. In addition, amylosucrase produces sucrose isomers such as turanose and trehalulose. It also efficiently synthesizes modified starch with increased ratios of slow digestive starch and resistant starch, and glucosylated functional compounds with increased water solubility and stability. It produces turnaose more efficiently than other carbohydrate-active enzymes. Amylose synthesized by amylosucrase forms microparticles and these can be utilized as biocompatible materials with various bio-applications, including drug delivery, chromatography, and bioanalytical sciences |
-, 756762 |
| 2.4.1.4 | synthesis |
immobilization enhances the efficiency of the glycosylation reaction and is therefore considered effective for industrial application in sustainable production of dihydroxybenzene glucosides |
-, 757420 |
| 2.4.1.4 | synthesis |
isoquercitrin (quercetin-3-O-beta-D-glucopyranoside) has diverse biological functions, such as anti-oxidant and anticancer activity, but its use is limited by poor solubility and bioavailability. Enzymatically modified isoquercitrin (EMIQ) is a mixture of transglycosylated isoquercitrins that have better solubility and bioavailability than do quercetin and isoquercitrin. Amylosucrase (ASase), has transglycosylation activity to produce EMIQ. Both enzymes produce a variety of EMIQs including isoquercitrin, isoquercitrin-glucoside, isoquercitrin-diglucoside, and isoquercitrin-triglucoside. The enzyme has a higher bioconversion yield from isoquercitrin to EMIQ (97%). The yield of soquercitrin-triglucoside, which is the most bioavailable form is 46%. The enzyme can be used to synthesize EMIQ in a simple and specific process |
-, 756620 |
| 2.4.1.4 | synthesis |
mutant enzyme R226A, that is activated by the products it forms and yields twice as much insoluble glucan and lower quantities of by-products as the wild-type enzyme is a very promising enzyme for industrial synthesis of amylose-like polymers |
659439 |
| 2.4.1.4 | synthesis |
mutant enzyme R226K/I228V/A289I/F290Y/E300I/V331T/Q437S/N439D/C445A only produces soluble oligosaccharides as no insoluble high molecular weight amylose is observed. The mutant enzyme is an attractive enzymatic tool that could offer interesting opportunities for the design of amylodextrins with controlled size |
756344 |
| 2.4.1.4 | synthesis |
potential of amylosucrase in the design of original carbohydrate-based dendritic nanoparticles |
672511 |
| 2.4.1.4 | synthesis |
potentiality of amylosucrase in the design of amylodextrins with controlled morphology, structure, and physicochemical properties |
658253 |
| 2.4.1.4 | synthesis |
the batch-feeding whole-cell biocatalysis by Amy-1 is a promising technology for alpha-arbutin production with enhanced yield and molar conversion rate |
-, 757357 |
