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Literature summary for 2.4.1.4 extracted from

  • Albenne, C.; Skov, L.K.; Mirza, O.; Gajhede, M.; Feller, G.; D'Amico, S.; Andre, G.; Potocki-Veronese, G.; van der Veen, B.A.; Monsan, P.; Remaud-Simeon, M.
    Molecular basis of the amylose-like polymer formation catalyzed by Neisseria polysaccharea amylosucrase (2004), J. Biol. Chem., 279, 726-734.
    View publication on PubMed

Application

Application Comment Organism
synthesis mutant enzyme R226A, that is activated by the products it forms and yields twice as much insoluble glucan and lower quantities of by-products as the wild-type enzyme is a very promising enzyme for industrial synthesis of amylose-like polymers Neisseria polysaccharea

Protein Variants

Protein Variants Comment Organism
D394A 23.5% of the wild-type activity, according to the initial rate of sucrose consumption, very poor ativation by glycogen Neisseria polysaccharea
R226A activated by the products it forms. The mutant yields twice as much insoluble glucan as the wild-type enzyme and leads to the production of lower quantities of by-products, mutant enzyme is strongly activated by glycogen Neisseria polysaccharea
R415A 4.3% of the activity compared with the wild-type enzyme. No synthesis of any insoluble modified glycogen Neisseria polysaccharea
R446A 15% of the wild-type activity, according to the initial rate of sucrose consumption, no synthesis of any insoluble modified glycogen Neisseria polysaccharea

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
sucrose + (1,4-alpha-D-glucosyl)n Neisseria polysaccharea amylosucrase initializes polymer formation by releasing, through sucrose hydrolysis, a glucose molecule that is subsequently used as the first acceptor molecule. Maltooligosaccharides of increasing size are produced and successively elongated at their nonreducing ends until they reached a critical size and concentration, causing precipitation D-fructose + (1,4-alpha-D-glucosyl)n+1
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Organism

Organism UniProt Comment Textmining
Neisseria polysaccharea
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Purification (Commentary)

Purification (Comment) Organism
wild-type and mutant enzymes Neisseria polysaccharea

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
sucrose
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Neisseria polysaccharea ?
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sucrose + (1,4-alpha-D-glucosyl)n amylosucrase initializes polymer formation by releasing, through sucrose hydrolysis, a glucose molecule that is subsequently used as the first acceptor molecule. Maltooligosaccharides of increasing size are produced and successively elongated at their nonreducing ends until they reached a critical size and concentration, causing precipitation Neisseria polysaccharea D-fructose + (1,4-alpha-D-glucosyl)n+1
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sucrose + glycogen
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Neisseria polysaccharea ?
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sucrose + maltoheptaose
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Neisseria polysaccharea ?
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