3.2.2.1 | dithiothreitol |
after 1 h pre-incubation at temperatures until 80°C, the enzyme remains completely stable even at high concentrations of dithiothreitol (0.7 M) whereas it becomes susceptible to the effect of the reducing agent as the temperature raises, after 1 h pre-incubation in the presence of 0.5 M, 0.6 M and 0.7 M dithiothreitol, IAG-NH retains 70%, 60% and 21% activity respectively |
-, 695873 |