EC Number |
Natural Substrates |
---|
3.4.14.14 | more |
MftB is not required for the reaction catalyzed by the enzyme |
3.4.14.14 | oxidatively decarboxylated precursor peptide MftA + H2O |
the enzyme catalyzes the hydrolysis of the peptide bond between Gly28 and Val29 |
3.4.14.14 | more |
the enzyme does not recognize the unmodified peptide MftA nor the intermediate MftA-glycyl-N-[(E)-2-(4-hydroxyphenyl)ethenyl]-L-valinamide |
3.4.14.14 | precursor peptide MftA-glycyl-3-amino-5-[(4-hydroxyphenyl)methyl]-4,4-dimethylpyrrolidin-2-one + H2O |
the enzyme is specific for MftA-glycyl-3-amino-5-[(4-hydroxyphenyl)methyl]-4,4-dimethylpyrrolidin-2-one |
3.4.14.14 | precursor peptide MftA-glycyl-3-amino-5-[(4-hydroxyphenyl)methyl]-4,4-dimethylpyrrolidin-2-one + H2O |
the enzyme selectively hydrolyzes the amide bond between Val29 and Gly28 of MftA*, liberating the putative carbon skeleton of mycofactocin, 3-amino-5-[(p-hydroxyphenyl) methyl]-4,4-dimethyl-2-pyrrolidinone |