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Information on EC 3.4.14.14 - [mycofactocin precursor peptide] peptidase
for references in articles please use BRENDA:EC3.4.14.14
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EC Tree 3 Hydrolases
3.4 Acting on peptide bonds (peptidases)
3.4.14 Dipeptidyl-peptidases and tripeptidyl-peptidases
3.4.14.14 [mycofactocin precursor peptide] peptidase
IUBMB Comments
Requires Fe2+ ad Zn2+. The enzyme participates in the biosynthesis of the enzyme cofactor mycofactocin. It catalyses cleavage of the mycofactocin precursor peptide following its modification by MftC to liberate its final two residues, which consist of a cross-linked valine-tyramine dipeptide.
The expected taxonomic range for this enzyme is: Mycobacteriaceae
Reaction Schemes
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C-terminal [mycofactocin precursor peptide]-glycyl-3-amino-5-[(4-hydroxyphenyl)methyl]-4,4-dimethylpyrrolidin-2-one
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C-terminal [mycofactocin precursor peptide]-glycine
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Synonyms
LI98_07105, mftE, MSMEG_1425, Rv0695, more
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SYNONYM 
ORGANISM
UNIPROT
COMMENTARY 
LITERATURE
LI98_07105
mftE
Rv0695
mftE
-
-
-
-
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REACTION
REACTION DIAGRAM
COMMENTARY
ORGANISM
UNIPROT
LITERATURE
C-terminal [mycofactocin precursor peptide]-glycyl-3-amino-5-[(4-hydroxyphenyl)methyl]-4,4-dimethylpyrrolidin-2-one + H2O = C-terminal [mycofactocin precursor peptide]-glycine + 3-amino-5-[(4-hydroxyphenyl)methyl]-4,4-dimethylpyrrolidin-2-one
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PATHWAY SOURCE
PATHWAYS
MetaCyc
mycofactocin biosynthesis
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SYSTEMATIC NAME
IUBMB Comments
C-terminal [mycofactocin precursor peptide]-glycyl-3-amino-5-[(4-hydroxyphenyl)methyl]-4,4-dimethylpyrrolidin-2-one 3-amino-5-[(4-hydroxyphenyl)methyl]-4,4-dimethylpyrrolidin-2-one hydrolyase
Requires Fe2+ ad Zn2+. The enzyme participates in the biosynthesis of the enzyme cofactor mycofactocin. It catalyses cleavage of the mycofactocin precursor peptide following its modification by MftC to liberate its final two residues, which consist of a cross-linked valine-tyramine dipeptide.
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SUBSTRATE
PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
LITERATURE
COMMENTARY
Reversibility
r=reversible
ir=irreversible
?=not specified
r=reversible
ir=irreversible
?=not specified
C-terminal [mycofactocin precursor peptide]-glycyl-3-amino-5-[(4-hydroxyphenyl)methyl]-4,4-dimethylpyrrolidin-2-one + H2O
C-terminal [mycofactocin precursor peptide]-glycine + 3-amino-5-[(4-hydroxyphenyl)methyl]-4,4-dimethylpyrrolidin-2-one
oxidatively decarboxylated precursor peptide MftA + H2O
precursor peptide MftA(1-28) + 3-amino-5-[(4-hydroxyphenyl)methyl]-4,4-dimethylpyrrolidin-2-one
precursor peptide MftA-glycyl-3-amino-5-[(4-hydroxyphenyl)methyl]-4,4-dimethylpyrrolidin-2-one + H2O
MftA(1-28) + 3-amino-5-[(4-hydroxyphenyl)methyl]-4,4-dimethyl-2-pyrrolidinone
precursor peptide MftA-glycyl-3-amino-5-[(4-hydroxyphenyl)methyl]-4,4-dimethylpyrrolidin-2-one + H2O
precursor peptide MftA(1-28) + 3-amino-5-[(4-hydroxyphenyl)methyl]-4,4-dimethyl-2-pyrrolidinone
C-terminal [mycofactocin precursor peptide]-glycyl-3-amino-5-[(4-hydroxyphenyl)methyl]-4,4-dimethylpyrrolidin-2-one + H2O
C-terminal [mycofactocin precursor peptide]-glycine + 3-amino-5-[(4-hydroxyphenyl)methyl]-4,4-dimethylpyrrolidin-2-one
Substrates: -
Products: -
Products: -
?
C-terminal [mycofactocin precursor peptide]-glycyl-3-amino-5-[(4-hydroxyphenyl)methyl]-4,4-dimethylpyrrolidin-2-one + H2O
C-terminal [mycofactocin precursor peptide]-glycine + 3-amino-5-[(4-hydroxyphenyl)methyl]-4,4-dimethylpyrrolidin-2-one
Substrates: -
Products: -
Products: -
?
C-terminal [mycofactocin precursor peptide]-glycyl-3-amino-5-[(4-hydroxyphenyl)methyl]-4,4-dimethylpyrrolidin-2-one + H2O
C-terminal [mycofactocin precursor peptide]-glycine + 3-amino-5-[(4-hydroxyphenyl)methyl]-4,4-dimethylpyrrolidin-2-one
Substrates: -
Products: -
Products: -
?
oxidatively decarboxylated precursor peptide MftA + H2O
precursor peptide MftA(1-28) + 3-amino-5-[(4-hydroxyphenyl)methyl]-4,4-dimethylpyrrolidin-2-one
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Substrates: the enzyme catalyzes the hydrolysis of the peptide bond between Gly28 and Val29
Products: -
Products: -
?
oxidatively decarboxylated precursor peptide MftA + H2O
precursor peptide MftA(1-28) + 3-amino-5-[(4-hydroxyphenyl)methyl]-4,4-dimethylpyrrolidin-2-one
Mycolicibacterium smegmatis ATCC 7000840
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Substrates: the enzyme catalyzes the hydrolysis of the peptide bond between Gly28 and Val29
Products: -
Products: -
?
precursor peptide MftA-glycyl-3-amino-5-[(4-hydroxyphenyl)methyl]-4,4-dimethylpyrrolidin-2-one + H2O
MftA(1-28) + 3-amino-5-[(4-hydroxyphenyl)methyl]-4,4-dimethyl-2-pyrrolidinone
Substrates: the enzyme selectively hydrolyzes the amide bond between Val29 and Gly28 of MftA*, liberating the putative carbon skeleton of mycofactocin, 3-amino-5-[(p-hydroxyphenyl) methyl]-4,4-dimethyl-2-pyrrolidinone
Products: -
Products: -
?
precursor peptide MftA-glycyl-3-amino-5-[(4-hydroxyphenyl)methyl]-4,4-dimethylpyrrolidin-2-one + H2O
MftA(1-28) + 3-amino-5-[(4-hydroxyphenyl)methyl]-4,4-dimethyl-2-pyrrolidinone
Substrates: the enzyme selectively hydrolyzes the amide bond between Val29 and Gly28 of MftA*, liberating the putative carbon skeleton of mycofactocin, 3-amino-5-[(p-hydroxyphenyl) methyl]-4,4-dimethyl-2-pyrrolidinone
Products: -
Products: -
?
precursor peptide MftA-glycyl-3-amino-5-[(4-hydroxyphenyl)methyl]-4,4-dimethylpyrrolidin-2-one + H2O
MftA(1-28) + 3-amino-5-[(4-hydroxyphenyl)methyl]-4,4-dimethyl-2-pyrrolidinone
-
Substrates: the enzyme is specific for MftA-glycyl-3-amino-5-[(4-hydroxyphenyl)methyl]-4,4-dimethylpyrrolidin-2-one
Products: -
Products: -
?
precursor peptide MftA-glycyl-3-amino-5-[(4-hydroxyphenyl)methyl]-4,4-dimethylpyrrolidin-2-one + H2O
MftA(1-28) + 3-amino-5-[(4-hydroxyphenyl)methyl]-4,4-dimethyl-2-pyrrolidinone
-
Substrates: the enzyme is specific for MftA-glycyl-3-amino-5-[(4-hydroxyphenyl)methyl]-4,4-dimethylpyrrolidin-2-one
Products: -
Products: -
?
precursor peptide MftA-glycyl-3-amino-5-[(4-hydroxyphenyl)methyl]-4,4-dimethylpyrrolidin-2-one + H2O
precursor peptide MftA(1-28) + 3-amino-5-[(4-hydroxyphenyl)methyl]-4,4-dimethyl-2-pyrrolidinone
-
Substrates: -
Products: -
Products: -
?
precursor peptide MftA-glycyl-3-amino-5-[(4-hydroxyphenyl)methyl]-4,4-dimethylpyrrolidin-2-one + H2O
precursor peptide MftA(1-28) + 3-amino-5-[(4-hydroxyphenyl)methyl]-4,4-dimethyl-2-pyrrolidinone
-
Substrates: -
Products: -
Products: -
?
additional information
?
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Substrates: the enzyme does not recognize the unmodified peptide MftA nor the intermediate MftA-glycyl-N-[(E)-2-(4-hydroxyphenyl)ethenyl]-L-valinamide
Products: -
Products: -
?
additional information
?
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Substrates: the enzyme does not recognize the unmodified peptide MftA nor the intermediate MftA-glycyl-N-[(E)-2-(4-hydroxyphenyl)ethenyl]-L-valinamide
Products: -
Products: -
?
additional information
?
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-
Substrates: MftB is not required for the reaction catalyzed by the enzyme
Products: -
Products: -
?
additional information
?
-
Mycolicibacterium smegmatis ATCC 7000840
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Substrates: MftB is not required for the reaction catalyzed by the enzyme
Products: -
Products: -
?
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NATURAL SUBSTRATE
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
LITERATURE
COMMENTARY
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
r=reversible
ir=irreversible
?=not specified
C-terminal [mycofactocin precursor peptide]-glycyl-3-amino-5-[(4-hydroxyphenyl)methyl]-4,4-dimethylpyrrolidin-2-one + H2O
C-terminal [mycofactocin precursor peptide]-glycine + 3-amino-5-[(4-hydroxyphenyl)methyl]-4,4-dimethylpyrrolidin-2-one
oxidatively decarboxylated precursor peptide MftA + H2O
precursor peptide MftA(1-28) + 3-amino-5-[(4-hydroxyphenyl)methyl]-4,4-dimethylpyrrolidin-2-one
precursor peptide MftA-glycyl-3-amino-5-[(4-hydroxyphenyl)methyl]-4,4-dimethylpyrrolidin-2-one + H2O
MftA(1-28) + 3-amino-5-[(4-hydroxyphenyl)methyl]-4,4-dimethyl-2-pyrrolidinone
precursor peptide MftA-glycyl-3-amino-5-[(4-hydroxyphenyl)methyl]-4,4-dimethylpyrrolidin-2-one + H2O
precursor peptide MftA(1-28) + 3-amino-5-[(4-hydroxyphenyl)methyl]-4,4-dimethyl-2-pyrrolidinone
C-terminal [mycofactocin precursor peptide]-glycyl-3-amino-5-[(4-hydroxyphenyl)methyl]-4,4-dimethylpyrrolidin-2-one + H2O
C-terminal [mycofactocin precursor peptide]-glycine + 3-amino-5-[(4-hydroxyphenyl)methyl]-4,4-dimethylpyrrolidin-2-one
Substrates: -
Products: -
Products: -
?
C-terminal [mycofactocin precursor peptide]-glycyl-3-amino-5-[(4-hydroxyphenyl)methyl]-4,4-dimethylpyrrolidin-2-one + H2O
C-terminal [mycofactocin precursor peptide]-glycine + 3-amino-5-[(4-hydroxyphenyl)methyl]-4,4-dimethylpyrrolidin-2-one
Substrates: -
Products: -
Products: -
?
C-terminal [mycofactocin precursor peptide]-glycyl-3-amino-5-[(4-hydroxyphenyl)methyl]-4,4-dimethylpyrrolidin-2-one + H2O
C-terminal [mycofactocin precursor peptide]-glycine + 3-amino-5-[(4-hydroxyphenyl)methyl]-4,4-dimethylpyrrolidin-2-one
Substrates: -
Products: -
Products: -
?
oxidatively decarboxylated precursor peptide MftA + H2O
precursor peptide MftA(1-28) + 3-amino-5-[(4-hydroxyphenyl)methyl]-4,4-dimethylpyrrolidin-2-one
-
Substrates: the enzyme catalyzes the hydrolysis of the peptide bond between Gly28 and Val29
Products: -
Products: -
?
oxidatively decarboxylated precursor peptide MftA + H2O
precursor peptide MftA(1-28) + 3-amino-5-[(4-hydroxyphenyl)methyl]-4,4-dimethylpyrrolidin-2-one
Mycolicibacterium smegmatis ATCC 7000840
-
Substrates: the enzyme catalyzes the hydrolysis of the peptide bond between Gly28 and Val29
Products: -
Products: -
?
precursor peptide MftA-glycyl-3-amino-5-[(4-hydroxyphenyl)methyl]-4,4-dimethylpyrrolidin-2-one + H2O
MftA(1-28) + 3-amino-5-[(4-hydroxyphenyl)methyl]-4,4-dimethyl-2-pyrrolidinone
Substrates: the enzyme selectively hydrolyzes the amide bond between Val29 and Gly28 of MftA*, liberating the putative carbon skeleton of mycofactocin, 3-amino-5-[(p-hydroxyphenyl) methyl]-4,4-dimethyl-2-pyrrolidinone
Products: -
Products: -
?
precursor peptide MftA-glycyl-3-amino-5-[(4-hydroxyphenyl)methyl]-4,4-dimethylpyrrolidin-2-one + H2O
MftA(1-28) + 3-amino-5-[(4-hydroxyphenyl)methyl]-4,4-dimethyl-2-pyrrolidinone
Substrates: the enzyme selectively hydrolyzes the amide bond between Val29 and Gly28 of MftA*, liberating the putative carbon skeleton of mycofactocin, 3-amino-5-[(p-hydroxyphenyl) methyl]-4,4-dimethyl-2-pyrrolidinone
Products: -
Products: -
?
precursor peptide MftA-glycyl-3-amino-5-[(4-hydroxyphenyl)methyl]-4,4-dimethylpyrrolidin-2-one + H2O
MftA(1-28) + 3-amino-5-[(4-hydroxyphenyl)methyl]-4,4-dimethyl-2-pyrrolidinone
-
Substrates: the enzyme is specific for MftA-glycyl-3-amino-5-[(4-hydroxyphenyl)methyl]-4,4-dimethylpyrrolidin-2-one
Products: -
Products: -
?
precursor peptide MftA-glycyl-3-amino-5-[(4-hydroxyphenyl)methyl]-4,4-dimethylpyrrolidin-2-one + H2O
MftA(1-28) + 3-amino-5-[(4-hydroxyphenyl)methyl]-4,4-dimethyl-2-pyrrolidinone
-
Substrates: the enzyme is specific for MftA-glycyl-3-amino-5-[(4-hydroxyphenyl)methyl]-4,4-dimethylpyrrolidin-2-one
Products: -
Products: -
?
precursor peptide MftA-glycyl-3-amino-5-[(4-hydroxyphenyl)methyl]-4,4-dimethylpyrrolidin-2-one + H2O
precursor peptide MftA(1-28) + 3-amino-5-[(4-hydroxyphenyl)methyl]-4,4-dimethyl-2-pyrrolidinone
-
Substrates: -
Products: -
Products: -
?
precursor peptide MftA-glycyl-3-amino-5-[(4-hydroxyphenyl)methyl]-4,4-dimethylpyrrolidin-2-one + H2O
precursor peptide MftA(1-28) + 3-amino-5-[(4-hydroxyphenyl)methyl]-4,4-dimethyl-2-pyrrolidinone
-
Substrates: -
Products: -
Products: -
?
additional information
?
-
-
Substrates: the enzyme does not recognize the unmodified peptide MftA nor the intermediate MftA-glycyl-N-[(E)-2-(4-hydroxyphenyl)ethenyl]-L-valinamide
Products: -
Products: -
?
additional information
?
-
-
Substrates: the enzyme does not recognize the unmodified peptide MftA nor the intermediate MftA-glycyl-N-[(E)-2-(4-hydroxyphenyl)ethenyl]-L-valinamide
Products: -
Products: -
?
additional information
?
-
-
Substrates: MftB is not required for the reaction catalyzed by the enzyme
Products: -
Products: -
?
additional information
?
-
Mycolicibacterium smegmatis ATCC 7000840
-
Substrates: MftB is not required for the reaction catalyzed by the enzyme
Products: -
Products: -
?
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METALS and IONS
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
Fe2+
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ORGANISM
COMMENTARY
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
Highest Expressing Human Cell Lines
Cell Line LinksPlease wait a moment until the data is sorted. This message will disappear when the data is sorted.
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
physiological function
physiological function
the enzyme is essential for in vitro growth in cholesterol
physiological function
the enzyme is essential for in vitro growth in cholesterol
physiological function
-
the enzyme is essential for in vitro growth in cholesterol
-
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UNIPROT
ENTRY NAME
ORGANISM
NO. OF AA
NO. OF TRANSM. HELICES
MOLECULAR WEIGHT[Da]
SOURCE
SEQUENCE
LOCALIZATION PREDICTION
The reliability class of the localization prediction ranges from 1 (very reliable) to 5 (not reliable).
The reliability class of the localization prediction ranges from 1 (very reliable) to 5 (not reliable). MFTE_MYCUA
Mycobacterium ulcerans (strain Agy99)
250
0
26103
Swiss-Prot
-
MFTE_MYCS2
Mycolicibacterium smegmatis (strain ATCC 700084 / mc(2)155)
247
0
25761
Swiss-Prot
Secretory Pathway (Reliability: 1)
MFTE_MYCTU
Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv)
251
0
26580
Swiss-Prot
-
MFTE_MYCTO
Mycobacterium tuberculosis (strain CDC 1551 / Oshkosh)
251
0
26580
Swiss-Prot
-
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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
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REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Ayikpoe, R.; Latham, J.
MftD catalyzes the formation of a biologically active redox center in the biosynthesis of the ribosomally synthesized and post-translationally modified redox cofactor mycofactocin
J. Am. Chem. Soc.
141
13582-13591
2019
Mycolicibacterium smegmatis, Mycolicibacterium smegmatis mc2155
brenda
Ayikpoe, R.; Govindarajan, V.; Latham, J.A.
Occurrence, function, and biosynthesis of mycofactocin
Appl. Microbiol. Biotechnol.
103
2903-2912
2019
Mycobacterium tuberculosis (P9WP59), Mycobacterium tuberculosis H37Rv (P9WP59)
brenda
Ayikpoe, R.; Salazar, J.; Majestic, B.; Latham, J.A.
Mycofactocin biosynthesis proceeds through 3-amino-5-[( p-hydroxyphenyl)methyl]-4,4-dimethyl-2-pyrrolidinone (AHDP); direct observation of MftE specificity toward MftA
Biochemistry
57
5379-5383
2018
Mycobacterium ulcerans, Mycolicibacterium smegmatis
brenda
Bruender, N.A.; Bandarian, V.
The creatininase homolog MftE from Mycobacterium smegmatis catalyzes a peptide cleavage reaction in the biosynthesis of a novel ribosomally synthesized post-translationally modified peptide (RiPP)
J. Biol. Chem.
292
4371-4381
2017
Mycolicibacterium smegmatis, Mycolicibacterium smegmatis ATCC 7000840
brenda
Krishnamoorthy, G.; Kaiser, P.; Lozza, L.; Hahnke, K.; Mollenkopf, H.; Kaufmann, S.
Mycofactocin is associated with ethanol metabolism in mycobacteria
mBio
10
e00190-19
2019
Mycobacterium tuberculosis (P9WP59), Mycobacterium tuberculosis H37Rv (P9WP59), Mycolicibacterium smegmatis (A0QSC0)
brenda
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