EC Number   |
Natural Substrates |
|---|
  1.14.14.178 | a C28 steroid + [reduced NADPH-hemoprotein reductase] + O2 |
- |
| 1.14.14.178 | more |
CYP724B2 function in the early steps of C-22 hydroxylation of brassinosteroid biosynthesis |
| 1.14.14.178 | more |
Arabidopsis plants ectopically overexpressing DWF4 (AOD4) are generated, using the cauliflower mosaic virus 35S promoter, and their phenotypes are characterized. The hypocotyl length of both light and dark-grown AOD4 seedlings is increased dramatically as compared to wild type. At maturity, inflorescence height increases >35% in AOD4 lines and >14% in tobacco DWF4 overexpressing lines (TOD4), relative to controls. The total number of branches and siliques increased more than twofold in AOD4 plants, leading to a 59% increase in the number of seeds produced |
| 1.14.14.178 | more |
DWF4 is down-regulated by jasmonate and is located downstream of COI1 in the jasmonate-signaling pathway |
| 1.14.14.178 | more |
only 22alpha-hydroxylated brassinosteroids rescue the dwf4 phenotype, confirming that DWF4 acts as a 22alpha-hydroxylase |
| 1.14.14.178 | more |
the 22-hydroxylation step is one of the rate-limiting steps in brassinosteroid biosynthesis |
| 1.14.14.178 | more |
the regulation of DWARF4 expression is likely a critical mechanism in maintaining the homeostasis of bioactive brassinosteroids in Arabidopsis |
