EC Number   |
Metals/Ions  |
Reference |
|---|
   3.5.1.103 | Co2+ |
activity follows the trend Fe2+>Co2+>Zn2+>Mn2+ and Ni2+ |
719933 |
| 3.5.1.103 | Co2+ |
MshB activity lost by incubation of the Ni enzyme with 1,10-phenanthroline can be restored following removal of 1,10-phenanthroline by incubation with 0.1 mM Zn2+, Ni2+, Mn2+, or Co2+, the latter promoting the highest activity. Ca2+ and Mg2+ produce no restoration of activity |
701051 |
| 3.5.1.103 | Co2+ |
MshB contains a divalent transition metal ion essential for activity. MshB isolated on the Ni-affinity column contains 0.36 equivalent of Ca and 0.82 equivalent of Ni, and less than 0.08 equivalent of Zn per subunit. MshB isolated on the Zn-affinity column contains less than 0.1 equivalent of Ca and 2.3 equivalents of Zn, and less than 0.08 equivalent of Ni per subunit. MshB activity lost by incubation of the Ni enzyme with 1,10-phenanthroline can be restored following removal of 1,10-phenanthroline by incubation with 0.1 mM Zn2+, Ni2+, Mn2+, or Co2+, the latter promoting the highest activity, but Ca2+ and Mg2+ produce no restoration of activity |
701051 |
| 3.5.1.103 | Fe2+ |
activity follows the trend Fe2+>Co2+>Zn2+>Mn2+ and Ni2+ |
719933 |
| 3.5.1.103 | Fe2+ |
the active site metal ion is coordinated to two histidine residues, His13 and His147, and an aspartate, Asp16 |
734822 |
| 3.5.1.103 | Mn2+ |
activity follows the trend Fe2+>Co2+>Zn2+>Mn2+ and Ni2+ |
719933 |
| 3.5.1.103 | Mn2+ |
MshB activity lost by incubation of the Ni enzyme with 1,10-phenanthroline can be restored following removal of 1,10-phenanthroline by incubation with 0.1 mM Zn2+, Ni2+, Mn2+, or Co2+, the latter promoting the highest activity. Ca2+ and Mg2+ produce no restoration of activity |
701051 |
| 3.5.1.103 | Mn2+ |
MshB contains a divalent transition metal ion essential for activity. MshB isolated on the Ni-affinity column contains 0.36 equivalent of Ca and 0.82 equivalent of Ni, and less than 0.08 equivalent of Zn per subunit. MshB isolated on the Zn-affinity column contains less than 0.1 equivalent of Ca and 2.3 equivalents of Zn, and less than 0.08 equivalent of Ni per subunit. MshB activity lost by incubation of the Ni enzyme with 1,10-phenanthroline can be restored following removal of 1,10-phenanthroline by incubation with 0.1 mM Zn2+, Ni2+, Mn2+, or Co2+, the latter promoting the highest activity, but Ca2+ and Mg2+ produce no restoration of activity |
701051 |
| 3.5.1.103 | more |
enzyme has a strong preference for the Fe2+ cofactor under anaerobic conditions, regardless of the metal ion content of the medium. The preferred cofactor changes between Zn2+ and Fe2+ depending on the metal ion content of the medium when MshB is purified under aerobic conditions |
719933 |
| 3.5.1.103 | Ni2+ |
activity follows the trend Fe2+>Co2+>Zn2+>Mn2+ and Ni2+ |
719933 |
