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Literature summary for 3.5.1.103 extracted from

  • Huang, X.; Kocabas, E.; Hernick, M.
    The activity and cofactor preferences of N-acetyl-1-D-myo-inosityl-2-amino-2-deoxy-alpha-D-glucopyranoside deacetylase (MshB) change depending on environmental conditions (2011), J. Biol. Chem., 286, 20275-20282.
    View publication on PubMedView publication on EuropePMC

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
34
-
N-acetyl-D-glucosamine Co2+-enzyme, pH 7.5, 30┬░C Mycolicibacterium smegmatis
36
-
N-acetyl-D-glucosamine apo-enzyme, pH 7.5, 30┬░C Mycolicibacterium smegmatis
41
-
N-acetyl-D-glucosamine Fe2+-enzyme, pH 7.5, 30┬░C Mycolicibacterium smegmatis
53
-
N-acetyl-D-glucosamine Ni2+-enzyme, pH 7.5, 30┬░C Mycolicibacterium smegmatis
54
-
N-acetyl-D-glucosamine Mn2+-enzyme, pH 7.5, 30┬░C Mycolicibacterium smegmatis
56
-
N-acetyl-D-glucosamine Fe3+-enzyme, pH 7.5, 30┬░C Mycolicibacterium smegmatis

Metals/Ions

Metals/Ions Comment Organism Structure
Co2+ activity follows the trend Fe2+>Co2+>Zn2+>Mn2+ and Ni2+ Mycolicibacterium smegmatis
Fe2+ activity follows the trend Fe2+>Co2+>Zn2+>Mn2+ and Ni2+ Mycolicibacterium smegmatis
Mn2+ activity follows the trend Fe2+>Co2+>Zn2+>Mn2+ and Ni2+ Mycolicibacterium smegmatis
additional information enzyme has a strong preference for the Fe2+ cofactor under anaerobic conditions, regardless of the metal ion content of the medium. The preferred cofactor changes between Zn2+ and Fe2+ depending on the metal ion content of the medium when MshB is purified under aerobic conditions Mycolicibacterium smegmatis
Ni2+ activity follows the trend Fe2+>Co2+>Zn2+>Mn2+ and Ni2+ Mycolicibacterium smegmatis
Zn2+ activity follows the trend Fe2+>Co2+>Zn2+>Mn2+ and Ni2+ Mycolicibacterium smegmatis

Organism

Organism UniProt Comment Textmining
Mycolicibacterium smegmatis
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
N-acetyl-1D-myo-inosityl-2-amino-2-deoxy-alpha-D-glucopyranoside + H2O
-
Mycolicibacterium smegmatis 1D-myo-inosityl-2-amino-2-deoxy-alpha-D-glucopyranoside + acetate
-
?
N-acetyl-D-glucosamine + H2O
-
Mycolicibacterium smegmatis D-glucosamine + acetate
-
?

Synonyms

Synonyms Comment Organism
MshB
-
Mycolicibacterium smegmatis

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.11
-
N-acetyl-D-glucosamine Fe3+-enzyme, pH 7.5, 30┬░C Mycolicibacterium smegmatis
0.13
-
N-acetyl-D-glucosamine apo-enzyme, pH 7.5, 30┬░C Mycolicibacterium smegmatis
0.27
-
N-acetyl-D-glucosamine Ni2+-enzyme, pH 7.5, 30┬░C Mycolicibacterium smegmatis
0.38
-
N-acetyl-D-glucosamine Mn2+-enzyme, pH 7.5, 30┬░C Mycolicibacterium smegmatis
1.1
-
N-acetyl-D-glucosamine Co2+-enzyme, pH 7.5, 30┬░C Mycolicibacterium smegmatis
2.1
-
N-acetyl-D-glucosamine Fe2+-enzyme, pH 7.5, 30┬░C Mycolicibacterium smegmatis

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
0.0018
-
N-acetyl-D-glucosamine Fe3+-enzyme, pH 7.5, 30┬░C Mycolicibacterium smegmatis
0.0036
-
N-acetyl-D-glucosamine apo-enzyme, pH 7.5, 30┬░C Mycolicibacterium smegmatis
0.0053
-
N-acetyl-D-glucosamine Ni2+-enzyme, pH 7.5, 30┬░C Mycolicibacterium smegmatis
0.007
-
N-acetyl-D-glucosamine Mn2+-enzyme, pH 7.5, 30┬░C Mycolicibacterium smegmatis
0.032
-
N-acetyl-D-glucosamine Co2+-enzyme, pH 7.5, 30┬░C Mycolicibacterium smegmatis
0.052
-
N-acetyl-D-glucosamine Fe2+-enzyme, pH 7.5, 30┬░C Mycolicibacterium smegmatis