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EC Number	Metals/Ions	Commentary	Reference
3.4.21.111	Ca2+	1 mol per mol enzyme	650294
3.4.21.111	Ca2+	binds to low-affinity Ca2+ binding site, binding is essential for thermostabilization	650604
3.4.21.111	Ca2+	required	731114
3.4.21.111	Ca2+	stabilizes, at least two Ca2+-binding sites, i. e. stronger and weaker binding sites, weaker binding site essential for heat stability of the enzyme, Ca2+ bound to the stronger binding site is hardly removed with EDTA	673906
3.4.21.111	La3+	binds to low-affinity Ca2+ binding site as well as to an additional metal ion-binding site that can also be involved in thermostabilization	650604
3.4.21.111	La3+	stabilizes	673906
3.4.21.111	Na+	binds to low-affinity Ca2+ binding site, binding is essential for thermostabilization	650604
3.4.21.111	Nd3+	stabilizes	673906
3.4.21.111	Sr2+	stabilizes	673906
3.4.21.111	Tb3+	stabilizes	673906

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Release 2023.1 (January 2023)