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Literature summary for 3.4.21.111 extracted from

  • Tanaka, T.; Matsuzawa, H.; Ohta, T.
    Substrate specificity of aqualysin I altered by an organic solvent, DMSO (1999), Biosci. Biotechnol. Biochem., 63, 446-448.
    View publication on PubMed
Search for more literature for 3.4.21.111

Cloned(Commentary)

Cloned (Comment) Organism
expression in Pichia pastoris Thermus aquaticus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.04
-
 N-succinyl-Phe-Ala-Ala-4-nitroanilide pH 7.5, 40°C, absence of DMSO Thermus aquaticus
0.064
-
 N-succinyl-Ile-Val-Ala-4-nitroanilide pH 7.5, 40°C, absence of DMSO Thermus aquaticus
1
-
 N-succinyl-Ile-Val-Ala-4-nitroanilide pH 7.5, 40°C, presence of DMSO Thermus aquaticus
1.1
-
 N-succinyl-Ala-Ala-Ala-4-nitroanilide pH 7.5, 40°C, absence of DMSO Thermus aquaticus
2.2
-
 N-succinyl-Phe-Ala-Ala-4-nitroanilide pH 7.5, 40°C, presence of DMSO Thermus aquaticus
9.3
-
 N-succinyl-Ala-Ala-Ala-4-nitroanilide pH 7.5, 40°C, presence of DMSO Thermus aquaticus

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ binds to low-affinity Ca2+ binding site, binding is essential for thermostabilization Thermus aquaticus
La3+ binds to low-affinity Ca2+ binding site as well as to an additional metal ion-binding site that can also be involved in thermostabilization Thermus aquaticus
Na+ binds to low-affinity Ca2+ binding site, binding is essential for thermostabilization Thermus aquaticus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
28000
-
 mature protein, SDS-PAGE Thermus aquaticus
38000
-
 precursor protein, SDS-PAGE Thermus aquaticus

Organic Solvent Stability

Organic Solvent Comment Organism
DMSO 10% DMSO alters the substrate specificity of the enzyme, solvent may take part in the substrate recognition of the enzyme Thermus aquaticus

Organism

Organism UniProt Comment Textmining
Thermus aquaticus
-
 YT-1
-
Thermus aquaticus YT-1
-
 YT-1
-

Posttranslational Modification

Posttranslational Modification Comment Organism
proteolytic modification intermolecular autocatalytic activation upon heat treatment at 70°C Thermus aquaticus

Purification (Commentary)

Purification (Comment) Organism
-
 Thermus aquaticus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
chromogenic succinyl-tripeptide-4-nitroanilide + H2O
-
 Thermus aquaticus ?
-
 ?
chromogenic succinyl-tripeptide-4-nitroanilide + H2O
-
 Thermus aquaticus YT-1 ?
-
 ?
N-succinyl-Ala-Ala-Ala-4-nitroanilide + H2O
-
 Thermus aquaticus N-succinyl-Ala-Ala-Ala + 4-nitroaniline
-
 ?
N-succinyl-Ala-Ala-Ala-4-nitroanilide + H2O
-
 Thermus aquaticus YT-1 N-succinyl-Ala-Ala-Ala + 4-nitroaniline
-
 ?
N-succinyl-Ile-Val-Ala-4-nitroanilide + H2O
-
 Thermus aquaticus N-succinyl-Ile-Val-Ala + 4-nitroaniline
-
 ?
N-succinyl-Ile-Val-Ala-4-nitroanilide + H2O
-
 Thermus aquaticus YT-1 N-succinyl-Ile-Val-Ala + 4-nitroaniline
-
 ?
N-succinyl-Phe-Ala-Ala-4-nitroanilide + H2O
-
 Thermus aquaticus N-succinyl-Phe-Ala-Ala + 4-nitroaniline
-
 ?
N-succinyl-Phe-Ala-Ala-4-nitroanilide + H2O
-
 Thermus aquaticus YT-1 N-succinyl-Phe-Ala-Ala + 4-nitroaniline
-
 ?

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.1
-
 N-succinyl-Phe-Ala-Ala-4-nitroanilide pH 7.5, 40°C, absence of DMSO Thermus aquaticus
0.18
-
 N-succinyl-Phe-Ala-Ala-4-nitroanilide pH 7.5, 40°C, presence of DMSO Thermus aquaticus
1.1
-
 N-succinyl-Ala-Ala-Ala-4-nitroanilide pH 7.5, 40°C, presence of DMSO Thermus aquaticus
1.3
-
 N-succinyl-Ala-Ala-Ala-4-nitroanilide pH 7.5, 40°C, absence of DMSO Thermus aquaticus
5
-
 N-succinyl-Ile-Val-Ala-4-nitroanilide pH 7.5, 40°C, absence of DMSO Thermus aquaticus
8.1
-
 N-succinyl-Ile-Val-Ala-4-nitroanilide pH 7.5, 40°C, presence of DMSO Thermus aquaticus